Enzymes Anusorn Cherdthong Ph D 137748 Applied Biochemistry

Enzymes Anusorn Cherdthong, Ph. D 137748 Applied Biochemistry in Nutritional Science Email: anusornc@kku. ac. th E-learning: http: //ags. kku. ac. th/e. Learning/137748

Introduction v. Enzymes are protein--<activated reaction v. Catabolism v. Anabolism v. Re-formation when end of reaction v. Gain the products

Role of enzyne Mc. Donald et al. (2011)

Type of enzymes v. Simple protein: biological activity without nonprotein part v. Conjugated protein or holoenzyme: biological activity with apoenzyme and cofactor

Classification of enzymes By The International Union of Biochemistry (IUB) v. Oxidoreductases v. Transferases v. Hydrolases v. Lyases v. Isomerases v. Ligases v. Synthase

Classification of enzymes Oxidoreductases: addition/remove H atom Ødehydrogenases ØOxidases ØPeroxidases ØCatalases ØOxygenases Øhydroxylases Ø(Riis, 1983; Mc. Donald et al. , 2011)

Classification of enzymes Transferases: group transfer ØTransaldolases ØTransketolases, acyl, glucosyl ØPhosphoryl transferases, kinases ØPhosphomutases

Classification of enzymes Hydrolases: activation of hydrolysis ØEsterasesglycosidases ØPeptidases ØPhosphatases ØThiolases ØPhospholipases ØAmidases ØDeaminases ØRibonucleases

Classification of enzymes Lyases: on double bond addition of water/ammonia/CO 2 ØAldolases ØHydratases ØDehydratases ØSynthases ØLyases

Classification of enzymes Isomerases: Group transfer to be an isomer v. Racemases v. Isomerases vmutases

Classification of enzymes Ligases or synthase: molecule formation with new bond Ø Pyruvate carboxylase

Property of enzymes ØHigh specificity on substrate ØAll enzymes are protein (12000 -1000000 da) activate on native conformation ØActivity of enzyme depend on amino acid group

Specificity enzymes Induced-fit theory The lock and key theory Mc. Donald et al. (2011)

Factor affecting on enzymes ØTemperate Øp. H ØConcentration of enzyme ØConcentration of substrate

Enzymes control ØNonactive form: pepsinogen etc ØCovalent modification: add phosphory group into AA group ØEnd product; inhibition on enzymes ØRegulatory protein: calmodulin

Enzymes inhibitor ØIrreversible inhibitor: iodoaceta mide ØReversible inhibitor Øcompetitive inhibitor Ønoncompetitive inhibitor Øuncompetitive inhibition

Enzymes in Animal nutrition v. Amylase v -galactozidase v. Cellulase v. Pectinase v. Protease vglucose oxidase v. Calatase v. Lipoxygenase

Conclusion Role of enzyme 1 2 Enzyme activity and control 3 Classification of enzyme

Thank you!