Enzyme Lab Background Enzymes are proteins that help

Enzyme Lab

Background • Enzymes are proteins that help chemical reactions occur at a faster rate by lowering the energy needed for the reactions • First, enzymes react with a substrate to form an enzyme-substrate complex (like lock and key) • Once complex is formed, the substrate becomes a product(s) and leaves the enzyme • Enzyme can then repeat reaction with more substrates • Enzymes are shaped so they will react with only one specific type of substrate

Enzymes only react with one specific type of substrate.

Objective • • • Cut out enzymes, substrates, and products Color code them Manipulate them Glue them Explain the reaction that occurs Complete parts A, B, C, D

Part A • Define the following terms: – Activation energy – Enzyme – Substrate – Catalyst – Active site – Product – Denatured

Part B • Cut out enzymes, substrates, and products • Organize cut outs on paper so pieces demonstrate this equation – enzyme + substrate enzyme-substrate complex enzyme + product 1 + product 2 • Color code them – Enzyme = purple – Substrate = green – Products = yellow and blue

Part B • Glue the cut outs in the appropriate places on the paper • Label the cut outs that you glued as the following compounds – Enzyme = Lactase – Substrate = Lactose – Products = Glucose and Galactose • Explain what happened (reaction) – Use and underline as many vocabulary words from Part A as you can

Part C • This time, demonstrate the following equation – enzyme + substrate 1 + substrate 2 enzymesubstrate complex enzyme + product • Color code them – Enzyme = purple – Substrates = yellow and red – Product = orange

Part C • Glue and label as the following compounds – Enzyme = Sucrase – Substrates = Glucose and Fructose – Product = Sucrose • Explain what happened (reaction) – Use and underline as many vocabulary words from Part A as you can

Part D • Enzymes work best at a certain temperature and p. H • Below or above this optimal temperature or optimal p. H, the reaction is slower • On the back of your paper, answer the following questions related to the reaction of hydrogen peroxide breaking down into water and oxygen

Part D • Using the data table provided, graph the data to determine the optimum temperature for the enzyme catalase which speeds up the reaction: H 2 O 2 H 2 O + O 2 – X-axis = temperature – Y-axis = reaction rate • Describe the line you drew; what happens as temperature increases? • What is the optimum temperature for which the enzyme activity is the greatest for the reaction?