Enzyme experiment Learning objectives Describe experiments to investigate

Enzyme experiment Learning objectives: • Describe experiments to investigate how enzyme activity can be affected by changes in temperature.

Enzyme experiment Task 1: Follow instructions on ‘Investigating effect of temperature on the activity of lipase’ worksheet. You will be assigned a particular temperature and are expected to collect repeat measurements. We will collate our data as a class. Temperature Time taken for reaction to occur Average Rate of (o. C) (seconds) reaction (s-1) 1 2 3 Average (1 ÷ average time )

Enzyme experiment Carry out this experiment: 1 2 3 4 5 10 ml hydrogen peroxide raw potato boiled potato copper (II) manganese oxide (VII) oxide

Enzyme experiment information Hydrogen peroxide is a toxic waste product found in living things. The body decomposes (breaks it down) to prevent it from harming cells and tissue. We call this chemical reaction: metabolism. Look at the results of your experiment, and describe what happened using the above vocabulary. hydrogen peroxide water + oxygen

Enzyme experiment results Decomposition normally happens very slowly Boiled potato does not make the reaction faster Raw potato makes hydrogen peroxide decompose quickly Manganese (VII) oxide makes the reaction happen faster Copper (II) oxide does not make the reaction faster

Thumbs up or down Learning objectives: • Describe experiments to investigate how enzyme activity can be affected by changes in temperature.

Experiments involving enzymes 1. Amylase and starch http: //www. nuffieldfoundation. org/practicalbiology/investigating-effect-ph-amylaseactivity 2. Catalase and hydrogen peroxide http: //www. nuffieldfoundation. org/practicalbiology/investigating-enzyme-controlledreaction-catalase-and-hydrogen-peroxideconcentrat