ENZYME CLASSIFICATION EXERCISE 1 GLUCOSE ATP GLUCOSE6 PHOSPHATE

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ENZYME CLASSIFICATION EXERCISE (1) GLUCOSE + ATP GLUCOSE-6 -PHOSPHATE + ADP + H+ (2)

ENZYME CLASSIFICATION EXERCISE (1) GLUCOSE + ATP GLUCOSE-6 -PHOSPHATE + ADP + H+ (2) CH 3 CH 2 OH + NAD+ (CH 3)CHO + NADH + H+ (3) ATP + H 2 O ADP + Pi (4) CH 3(CO)COO- + H+ CH 3 CHO + (PYRUVATE) (ACETALDEHYDE) CO 2 (5) CH 3(CO)COO- + ATP + CO 2 ADP + Pi + -OOC-CH 2 (C 0) COO(OXALOACETATE) -OOC (6) H | | C = C | | H COO- H H | | C=C | | -OOC COO-

ENZYME CLASSIFICATION EXERCISE (7) OXALOACETATE + GTP PHOSPHOENOLPYRUVATE + GDP + CO 2 (8)

ENZYME CLASSIFICATION EXERCISE (7) OXALOACETATE + GTP PHOSPHOENOLPYRUVATE + GDP + CO 2 (8) ASP + -KETOGLUTARATE OXALOACETATE + GLU (9) 3 -PHOSPHOGLYCERATE 2 -PHOSPHOGLYCERATE (10) -OOC-CH 2 -COO- + FAD -OOC-CH=CH-COO- + FADH 2

ENZYME CLASSIFICATION ANSWERS TO IN-CLASS EXERCISE (1) TRANSFERASE (HEXOKINASE) (2) OXIDOREDUCTASE (ALCOHOL DEHYDROGENASE) (3)

ENZYME CLASSIFICATION ANSWERS TO IN-CLASS EXERCISE (1) TRANSFERASE (HEXOKINASE) (2) OXIDOREDUCTASE (ALCOHOL DEHYDROGENASE) (3) HYDROLASE (ATPase) (4) LYASE (PYRUVATE DECARBOXYLASE) (5) LIGASE (PYRUVATE CARBOXYLASE) (6) ISOMERASE (MALEATE ISOMERASE) (7) HYDROLASE (PHOSPHOENOLPYRUVATE CARBOXYKINASE) (8) TRANSFERASE (AMINOTRANSFERASE) (9) PHOSPHOGLYCERATE MUTASE (10) OXIDOREDUCTASE (SUCCINATE DEHYDROGENASE)

OXIDOREDUCTASES CATALYZE REDOX REACTIONS SOME COMMON TYPES: – – REDUCTASES DEHYDROGENASES OXIDASES OXYGENASES LOOK

OXIDOREDUCTASES CATALYZE REDOX REACTIONS SOME COMMON TYPES: – – REDUCTASES DEHYDROGENASES OXIDASES OXYGENASES LOOK FOR INVOLVEMENT OF COENZYMES – NAD+ , NADP+, FAD, FMN

TRANSFERASES TRANSFER OF FUNCTIONAL GROUPS SOME EXAMPLES: – TRANSFERASES – PHOSPHORYLASES TRANSFER OF Pi

TRANSFERASES TRANSFER OF FUNCTIONAL GROUPS SOME EXAMPLES: – TRANSFERASES – PHOSPHORYLASES TRANSFER OF Pi OR PPi – KINASES A PHOSPHATE TRANSFER UTILIZE OR GENERATE ATP

HYDROLASES HYDROLYSIS REACTIONS WATER MOLECULES USED TO BREAK BONDS EXAMPLES: – PHOSPHATASES – PEPTIDASES

HYDROLASES HYDROLYSIS REACTIONS WATER MOLECULES USED TO BREAK BONDS EXAMPLES: – PHOSPHATASES – PEPTIDASES – ATPase, GTPase

LYASES CATALYZES REACTIONS THAT – GENERATE A DOUBLE BOND – ADDS A SUBSTRATE MOLECULE

LYASES CATALYZES REACTIONS THAT – GENERATE A DOUBLE BOND – ADDS A SUBSTRATE MOLECULE TO DOUBLE BOND OF A SECOND SUBSTRATE EXAMPLES: – DECARBOXYLASES – DEHYDRATASES – ALDOLASE

ISOMERASES CONVERSION OF ONE ISOMERIC FORM INTO ANOTHER EXAMPLES: – – ISOMERASES EPIMERASES RACEMASES

ISOMERASES CONVERSION OF ONE ISOMERIC FORM INTO ANOTHER EXAMPLES: – – ISOMERASES EPIMERASES RACEMASES MUTASES

LIGASES TWO MOLECULES ARE JOINED ANABOLIC REACTIONS REQUIRE NUCLEOTIDES (ATP, GTP) TO DRIVE THEM

LIGASES TWO MOLECULES ARE JOINED ANABOLIC REACTIONS REQUIRE NUCLEOTIDES (ATP, GTP) TO DRIVE THEM – A PYROPHOSPHATE BOND MUST BE BROKEN EXAMPLES: – – CARBOXYLASE SYNTHETASES DON’T CONFUSE SYNTHETASES WITH SYNTHASES

ONGOING EXERCISE USING THE PRECEEDING PRINCIPLES OF ENZYME CLASSIFICATION, MAKE SURE THAT YOU CAN

ONGOING EXERCISE USING THE PRECEEDING PRINCIPLES OF ENZYME CLASSIFICATION, MAKE SURE THAT YOU CAN IDENTIFY THE CLASS OF ENZYME FOR EACH ENZYME THAT WE STUDY AND RATIONALIZE WHY IT FITS INTO THAT PARTICULAR CLASS

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