ENZYME ACTIVITY How to interpret enzyme graphs NEXT
- Slides: 72
ENZYME ACTIVITY How to interpret enzyme graphs (NEXT SLIDE)
Reading Graphs Substrate Which graph shows the amount of substrate going to zero faster? Substrate n Time (sec)
So… Substrate Nope, the amount of substrate gets to zero in less time in the OTHER graph. Substrate n Time (sec)
So… Substrate YES!! The amount of substrate gets to zero in less time in this graph. Substrate n Time (sec)
So… Substrate Which enzyme works FASTER? Substrate n Time (sec)
So… Substrate Nope, not this one. The faster the enzyme works, the faster the substrate will decrease. Substrate n Time (sec)
So… Substrate YES!! This enzyme works faster because the substrate decreases faster!! Substrate n Time (sec)
What do you think can affect the speed of an enzyme? TEMPERATURE p. H AMOUNT OF LIGHT
What do you think can affect the speed of an enzyme? TEMPERATURE n RIGHT!! Changing the p. H of the reaction can affect the speed of an enzyme. n ANYTHING ELSE? p. H AMOUNT OF LIGHT
What do you think can affect the speed of an enzyme? TEMPERATURE p. H AMOUNT OF LIGHT n RIGHT!! Changing the temperature of the reaction can ALSO affect the speed of an enzyme.
What do you think can affect the speed of an enzyme? TEMPERATURE n RIGHT!! Changing the temperature of the reaction can affect the speed of an enzyme. n ANYTHING ELSE? p. H AMOUNT OF LIGHT
What do you think can affect the speed of an enzyme? TEMPERATURE p. H AMOUNT OF LIGHT n RIGHT!! Changing the p. H of the reaction can ALSO affect the speed of an enzyme.
What do you think can affect the speed of an enzyme? TEMPERATURE n Sorry, most enzymes are NOT affected by the amount of light… n Pick again p. H AMOUNT OF LIGHT
Factors affecting enzyme speed n We can make a graph of enzyme speed at different temperatures.
Graph of ENZYME SPEED What should go on the X axis? Speed of enzyme Temperature ( C)
Graph of ENZYME SPEED Nope, the speed of the enzyme is what we are MEASURING, it should go on the Y axis. Speed of enzyme Temperature ( C)
Graph of ENZYME SPEED Amount of substrate What should go on the Y axis? (HINT: This is what we are MEASURING) Speed of enzyme RIGHT! Temperature should go on the X axis. 0 10 20 30 40 Temperature ( C) 50 60
Graph of ENZYME SPEED Amount of substrate Speed of enzyme 0 10 What should go on the Y axis? (HINT: This is what we are MEASURING) • We’re not measuring the amount of substrate this time. 20 30 40 Temperature (� C) 50 60
Graph of ENZYME SPEED FAST Speed of enzyme RIGHT!! The speed of enzyme should go on the Y axis. REMEMBER!! The enzyme is working FASTER As you go UP the Y axis. SLOW 0 10 20 30 40 Temperature (� C) 50 60
Let’s GRAPH!! FAST Speed of enzyme Amylase is found in saliva. Click on the red button that is approximately at body temperature SLOW 0 10 20 30 40 Temperature (� C) 50 60
Let’s GRAPH!! FAST Speed of enzyme Amylase is found in saliva. 5 C is close to freezing. TOO COLD!! SLOW 0 10 20 30 40 Temperature (� C) 50 60
Let’s GRAPH!! FAST Speed of enzyme Amylase is found in saliva. 22 C is close to room temperature. TOO COLD!! SLOW 0 10 20 30 40 Temperature (� C) 50 60
Let’s GRAPH!! FAST Speed of enzyme Amylase is found in saliva. 55 C is 131 F. TOO HOT!! SLOW 0 10 20 30 40 Temperature (� C) 50 60
Let’s GRAPH!! FAST Speed of enzyme Amylase is found in saliva. 37 C is body temperature. RIGHT!! Amylase will work best at about 37 C because it is normally found in your mouth SLOW 0 10 20 30 40 Temperature (� C) 50 60
Let’s GRAPH!! Speed of enzyme FAST Amylase works slowly At very low temperatures Because it is not close To body temperature SLOW 0 10 20 30 40 Temperature (� C) 50 60
Let’s GRAPH!! Speed of enzyme FAST Amylase will work Faster as it gets Closer to body Temperature. SLOW 0 10 20 30 40 Temperature (� C) 50 60
Let’s GRAPH!! FAST Speed of enzyme Amylase will work fastest at body Temperature. The temperature where Amylase works BEST is called the OPTIMUM TEMPERATURE SLOW 0 10 20 30 40 Temperature (� C) 50 60
Let’s GRAPH!! As the temperature gets too hot, Amylase doesn’t work as well. Speed of enzyme FAST SLOW 0 10 20 30 40 Temperature (� C) 50 60
Let’s GRAPH!! Click on the OPTIMUM TEMPERATURE Speed of enzyme FAST SLOW 0 10 20 30 40 Temperature (� C) 50 60
Let’s GRAPH!! Click on the OPTIMUM TEMPERATURE Speed of enzyme FAST RIGHT!! SLOW 0 10 20 30 40 Temperature (� C) 50 60
What is the optimum temperature for this enzyme? Speed of enzyme FAST 10 C 20 C 30 C SLOW 0 10 20 30 40 50 Temperature (� C) 60
What is the optimum temperature for this enzyme? FAST Speed of enzyme REMEMBER: The OPTIMUM is the temperature the enzyme works fastest at. 10 C 20 C 30 C SLOW 0 10 20 30 40 50 Temperature (� C) 60
What is the optimum temperature for this enzyme? FAST Speed of enzyme REMEMBER: The OPTIMUM is the temperature the enzyme works fastest at. 10 C 20 C 30 C SLOW 0 10 20 30 40 50 Temperature (� C) 60
Right!!
What is the optimum p. H for this enzyme? Speed of enzyme FAST 9 10 11 SLOW 0 2 4 p. H 6 8 10 12
What is the optimum p. H for this enzyme? FAST Speed of enzyme Look closely… 9 10 11 SLOW 0 2 4 p. H 6 8 10 12
What is the optimum p. H for this enzyme? FAST Speed of enzyme Look closely… 9 10 11 SLOW 0 2 4 p. H 6 8 10 12
Right!!
What is the optimum temperature for this enzyme? Speed of enzyme FAST 20 C 63 C 48 C SLOW 20 30 40 50 60 70 Temperature (� C) 80
What is the optimum temperature for this enzyme? Speed of enzyme FAST 20 C 63 C 48 C SLOW 20 30 40 50 60 70 Temperature (� C) 80
What is the optimum temperature for this enzyme? Speed of enzyme FAST 20 C 63 C 48 C SLOW 20 30 40 50 60 70 Temperature (� C) 80
Right!!
Temperature = 25 C 30 C 35 C Substrate What temperature does the enzyme work best at? Time At 25 C Time At 30 C At 35 C
Temperature = 25 C 30 C 35 C Substrate REMEMBER: the faster the substrate decreases, the faster the enzyme is working Time At 25 C Time At 30 C At 35 C
Temperature = 25 C 30 C 35 C Substrate REMEMBER: the faster the substrate decreases, the faster the enzyme is working Time At 25 C Time At 30 C At 35 C
TO SHOW WELL YOU UNDERSTOOD, COMPLETE THIS SHORT QUIZ
QUIZ - QUESTION 1 n Where in your body is the amylase enzyme found? SALIVA LIVER STOMACH
QUIZ - QUESTION 1 n Where in your body is the amylase enzyme found? SALIVA LIVER STOMACH
QUIZ - QUESTION 1 n Where in your body is the amylase enzyme found? SALIVA LIVER STOMACH
Right!!
QUIZ - QUESTION 2 n Click on the CATALYST in this reaction: SUGAR STARCH AMYLASE
QUIZ - QUESTION 2 n REMEMBER: The catalyst performs the reaction, but isn’t used up SUGAR STARCH AMYLASE
QUIZ - QUESTION 2 n REMEMBER: The catalyst performs the reaction, but isn’t used up SUGAR STARCH AMYLASE
Right!!
QUIZ - QUESTION 3 n Click on the substrate that is most likely to react with the enzyme below: STARCH PROTEIN LIPID
QUIZ - QUESTION 3 n REMEMBER: The subtrate that fits into a protein will be the one that reacts with it. STARCH PROTEIN LIPID
QUIZ - QUESTION 3 n REMEMBER: The subtrate that fits into a protein will be the one that reacts with it. STARCH PROTEIN LIPID
Right!! PROTEIN
QUIZ - QUESTION 4 n What is the most likely name of the enzyme in question 3 that reacts with protein? PROTEINOSE PROTEINATION PROTEINZYME PROTEINASE
QUIZ - QUESTION 4 n NOPE: sugars end in -OSE, not enzymes (sucrose, lactose, glucose) PROTEINOSE PROTEINATION PROTEINZYME PROTEINASE
QUIZ - QUESTION 4 n NOPE: think about “lactase” and “amylase” PROTEINOSE PROTEINATION PROTEINZYME PROTEINASE
QUIZ - QUESTION 4 n NOPE: think about “lactase” and “amylase” PROTEINOSE PROTEINATION PROTEINZYME PROTEINASE
Right!!
QUIZ - QUESTION 5 Click on the part of the graph that shows the OPTIMAL temperature for this enzyme Enzyme Activity n Temperature ( C)
QUIZ - QUESTION 5 REMEMBER: the OPTIMAL temperature for an enzyme is the temperature where the enzyme works BEST (highest activity) Enzyme Activity n Temperature ( C)
Right!!
QUIZ - QUESTION 6 Click on the graph that shows the optimum p. H for this enzyme. p. H = 7. 0 Substrate p. H = 5. 0 Time p. H = 3. 0 Substrate n Time
QUIZ - QUESTION 6 REMEMBER: Substrate decreases in LESS time if the enzyme works faster p. H = 7. 0 Substrate p. H = 5. 0 Time p. H = 3. 0 Substrate n Time
QUIZ - QUESTION 6 REMEMBER: Substrate decreases in LESS time if the enzyme works faster p. H = 7. 0 Substrate p. H = 5. 0 Time p. H = 3. 0 Substrate n Time
Right!!
Right!!
You have successfully completed the enzyme powerpoint.
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