ENZYME ACTIVITY How to interpret enzyme graphs NEXT

Reading Graphs Substrate Which graph shows the amount of substrate going to zero faster?

So… Substrate Nope, the amount of substrate gets to zero in less time in

So… Substrate YES!! The amount of substrate gets to zero in less time in

So… Substrate Which enzyme works FASTER? Substrate n Time (sec)

So… Substrate Nope, not this one. The faster the enzyme works, the faster the

So… Substrate YES!! This enzyme works faster because the substrate decreases faster!! Substrate n

What do you think can affect the speed of an enzyme? TEMPERATURE p. H

What do you think can affect the speed of an enzyme? TEMPERATURE n RIGHT!!

What do you think can affect the speed of an enzyme? TEMPERATURE n Sorry,

Factors affecting enzyme speed n We can make a graph of enzyme speed at

Graph of ENZYME SPEED What should go on the X axis? Speed of enzyme

Graph of ENZYME SPEED Nope, the speed of the enzyme is what we are

Graph of ENZYME SPEED Amount of substrate What should go on the Y axis?

Graph of ENZYME SPEED Amount of substrate Speed of enzyme 0 10 What should

Graph of ENZYME SPEED FAST Speed of enzyme RIGHT!! The speed of enzyme should

Let’s GRAPH!! FAST Speed of enzyme Amylase is found in saliva. Click on the

Let’s GRAPH!! FAST Speed of enzyme Amylase is found in saliva. 5 C is

Let’s GRAPH!! FAST Speed of enzyme Amylase is found in saliva. 22 C is

Let’s GRAPH!! FAST Speed of enzyme Amylase is found in saliva. 55 C is

Let’s GRAPH!! FAST Speed of enzyme Amylase is found in saliva. 37 C is

Let’s GRAPH!! Speed of enzyme FAST Amylase works slowly At very low temperatures Because

Let’s GRAPH!! Speed of enzyme FAST Amylase will work Faster as it gets Closer

Let’s GRAPH!! FAST Speed of enzyme Amylase will work fastest at body Temperature. The

Let’s GRAPH!! As the temperature gets too hot, Amylase doesn’t work as well. Speed

Let’s GRAPH!! Click on the OPTIMUM TEMPERATURE Speed of enzyme FAST SLOW 0 10

Let’s GRAPH!! Click on the OPTIMUM TEMPERATURE Speed of enzyme FAST RIGHT!! SLOW 0

What is the optimum temperature for this enzyme? Speed of enzyme FAST 10 C

What is the optimum temperature for this enzyme? FAST Speed of enzyme REMEMBER: The

What is the optimum p. H for this enzyme? Speed of enzyme FAST 9

What is the optimum p. H for this enzyme? FAST Speed of enzyme Look

What is the optimum temperature for this enzyme? Speed of enzyme FAST 20 C

Temperature = 25 C 30 C 35 C Substrate What temperature does the enzyme

Temperature = 25 C 30 C 35 C Substrate REMEMBER: the faster the substrate

TO SHOW WELL YOU UNDERSTOOD, COMPLETE THIS SHORT QUIZ

QUIZ - QUESTION 1 n Where in your body is the amylase enzyme found?

QUIZ - QUESTION 2 n Click on the CATALYST in this reaction: SUGAR STARCH

QUIZ - QUESTION 2 n REMEMBER: The catalyst performs the reaction, but isn’t used

QUIZ - QUESTION 3 n Click on the substrate that is most likely to

QUIZ - QUESTION 3 n REMEMBER: The subtrate that fits into a protein will

QUIZ - QUESTION 4 n What is the most likely name of the enzyme

QUIZ - QUESTION 4 n NOPE: sugars end in -OSE, not enzymes (sucrose, lactose,

QUIZ - QUESTION 4 n NOPE: think about “lactase” and “amylase” PROTEINOSE PROTEINATION PROTEINZYME

QUIZ - QUESTION 5 Click on the part of the graph that shows the

QUIZ - QUESTION 5 REMEMBER: the OPTIMAL temperature for an enzyme is the temperature

QUIZ - QUESTION 6 Click on the graph that shows the optimum p. H

QUIZ - QUESTION 6 REMEMBER: Substrate decreases in LESS time if the enzyme works

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ENZYME ACTIVITY How to interpret enzyme graphs (NEXT SLIDE)

Reading Graphs Substrate Which graph shows the amount of substrate going to zero faster? Substrate n Time (sec)

So… Substrate Nope, the amount of substrate gets to zero in less time in the OTHER graph. Substrate n Time (sec)

So… Substrate YES!! The amount of substrate gets to zero in less time in this graph. Substrate n Time (sec)

So… Substrate Which enzyme works FASTER? Substrate n Time (sec)

So… Substrate Nope, not this one. The faster the enzyme works, the faster the substrate will decrease. Substrate n Time (sec)

So… Substrate YES!! This enzyme works faster because the substrate decreases faster!! Substrate n Time (sec)

What do you think can affect the speed of an enzyme? TEMPERATURE p. H AMOUNT OF LIGHT

What do you think can affect the speed of an enzyme? TEMPERATURE n RIGHT!! Changing the p. H of the reaction can affect the speed of an enzyme. n ANYTHING ELSE? p. H AMOUNT OF LIGHT

What do you think can affect the speed of an enzyme? TEMPERATURE p. H AMOUNT OF LIGHT n RIGHT!! Changing the temperature of the reaction can ALSO affect the speed of an enzyme.

What do you think can affect the speed of an enzyme? TEMPERATURE n RIGHT!! Changing the temperature of the reaction can affect the speed of an enzyme. n ANYTHING ELSE? p. H AMOUNT OF LIGHT

What do you think can affect the speed of an enzyme? TEMPERATURE p. H AMOUNT OF LIGHT n RIGHT!! Changing the p. H of the reaction can ALSO affect the speed of an enzyme.

What do you think can affect the speed of an enzyme? TEMPERATURE n Sorry, most enzymes are NOT affected by the amount of light… n Pick again p. H AMOUNT OF LIGHT

Factors affecting enzyme speed n We can make a graph of enzyme speed at different temperatures.

Graph of ENZYME SPEED What should go on the X axis? Speed of enzyme Temperature ( C)

Graph of ENZYME SPEED Nope, the speed of the enzyme is what we are MEASURING, it should go on the Y axis. Speed of enzyme Temperature ( C)

Graph of ENZYME SPEED Amount of substrate What should go on the Y axis? (HINT: This is what we are MEASURING) Speed of enzyme RIGHT! Temperature should go on the X axis. 0 10 20 30 40 Temperature ( C) 50 60

Graph of ENZYME SPEED Amount of substrate Speed of enzyme 0 10 What should go on the Y axis? (HINT: This is what we are MEASURING) • We’re not measuring the amount of substrate this time. 20 30 40 Temperature (� C) 50 60

Graph of ENZYME SPEED FAST Speed of enzyme RIGHT!! The speed of enzyme should go on the Y axis. REMEMBER!! The enzyme is working FASTER As you go UP the Y axis. SLOW 0 10 20 30 40 Temperature (� C) 50 60

Let’s GRAPH!! FAST Speed of enzyme Amylase is found in saliva. Click on the red button that is approximately at body temperature SLOW 0 10 20 30 40 Temperature (� C) 50 60

Let’s GRAPH!! FAST Speed of enzyme Amylase is found in saliva. 5 C is close to freezing. TOO COLD!! SLOW 0 10 20 30 40 Temperature (� C) 50 60

Let’s GRAPH!! FAST Speed of enzyme Amylase is found in saliva. 22 C is close to room temperature. TOO COLD!! SLOW 0 10 20 30 40 Temperature (� C) 50 60

Let’s GRAPH!! FAST Speed of enzyme Amylase is found in saliva. 55 C is 131 F. TOO HOT!! SLOW 0 10 20 30 40 Temperature (� C) 50 60

Let’s GRAPH!! FAST Speed of enzyme Amylase is found in saliva. 37 C is body temperature. RIGHT!! Amylase will work best at about 37 C because it is normally found in your mouth SLOW 0 10 20 30 40 Temperature (� C) 50 60

Let’s GRAPH!! Speed of enzyme FAST Amylase works slowly At very low temperatures Because it is not close To body temperature SLOW 0 10 20 30 40 Temperature (� C) 50 60

Let’s GRAPH!! Speed of enzyme FAST Amylase will work Faster as it gets Closer to body Temperature. SLOW 0 10 20 30 40 Temperature (� C) 50 60

Let’s GRAPH!! FAST Speed of enzyme Amylase will work fastest at body Temperature. The temperature where Amylase works BEST is called the OPTIMUM TEMPERATURE SLOW 0 10 20 30 40 Temperature (� C) 50 60

Let’s GRAPH!! As the temperature gets too hot, Amylase doesn’t work as well. Speed of enzyme FAST SLOW 0 10 20 30 40 Temperature (� C) 50 60

Let’s GRAPH!! Click on the OPTIMUM TEMPERATURE Speed of enzyme FAST SLOW 0 10 20 30 40 Temperature (� C) 50 60

Let’s GRAPH!! Click on the OPTIMUM TEMPERATURE Speed of enzyme FAST RIGHT!! SLOW 0 10 20 30 40 Temperature (� C) 50 60

What is the optimum temperature for this enzyme? Speed of enzyme FAST 10 C 20 C 30 C SLOW 0 10 20 30 40 50 Temperature (� C) 60

What is the optimum temperature for this enzyme? FAST Speed of enzyme REMEMBER: The OPTIMUM is the temperature the enzyme works fastest at. 10 C 20 C 30 C SLOW 0 10 20 30 40 50 Temperature (� C) 60

Right!!

What is the optimum p. H for this enzyme? Speed of enzyme FAST 9 10 11 SLOW 0 2 4 p. H 6 8 10 12

What is the optimum p. H for this enzyme? FAST Speed of enzyme Look closely… 9 10 11 SLOW 0 2 4 p. H 6 8 10 12

Right!!

What is the optimum temperature for this enzyme? Speed of enzyme FAST 20 C 63 C 48 C SLOW 20 30 40 50 60 70 Temperature (� C) 80

Right!!

Temperature = 25 C 30 C 35 C Substrate What temperature does the enzyme work best at? Time At 25 C Time At 30 C At 35 C

Temperature = 25 C 30 C 35 C Substrate REMEMBER: the faster the substrate decreases, the faster the enzyme is working Time At 25 C Time At 30 C At 35 C

TO SHOW WELL YOU UNDERSTOOD, COMPLETE THIS SHORT QUIZ

QUIZ - QUESTION 1 n Where in your body is the amylase enzyme found? SALIVA LIVER STOMACH

Right!!

QUIZ - QUESTION 2 n Click on the CATALYST in this reaction: SUGAR STARCH AMYLASE

QUIZ - QUESTION 2 n REMEMBER: The catalyst performs the reaction, but isn’t used up SUGAR STARCH AMYLASE

Right!!

QUIZ - QUESTION 3 n Click on the substrate that is most likely to react with the enzyme below: STARCH PROTEIN LIPID

QUIZ - QUESTION 3 n REMEMBER: The subtrate that fits into a protein will be the one that reacts with it. STARCH PROTEIN LIPID

Right!! PROTEIN

QUIZ - QUESTION 4 n What is the most likely name of the enzyme in question 3 that reacts with protein? PROTEINOSE PROTEINATION PROTEINZYME PROTEINASE

QUIZ - QUESTION 4 n NOPE: sugars end in -OSE, not enzymes (sucrose, lactose, glucose) PROTEINOSE PROTEINATION PROTEINZYME PROTEINASE

QUIZ - QUESTION 4 n NOPE: think about “lactase” and “amylase” PROTEINOSE PROTEINATION PROTEINZYME PROTEINASE

Right!!

QUIZ - QUESTION 5 Click on the part of the graph that shows the OPTIMAL temperature for this enzyme Enzyme Activity n Temperature ( C)

QUIZ - QUESTION 5 REMEMBER: the OPTIMAL temperature for an enzyme is the temperature where the enzyme works BEST (highest activity) Enzyme Activity n Temperature ( C)

Right!!

QUIZ - QUESTION 6 Click on the graph that shows the optimum p. H for this enzyme. p. H = 7. 0 Substrate p. H = 5. 0 Time p. H = 3. 0 Substrate n Time

QUIZ - QUESTION 6 REMEMBER: Substrate decreases in LESS time if the enzyme works faster p. H = 7. 0 Substrate p. H = 5. 0 Time p. H = 3. 0 Substrate n Time

Right!!

You have successfully completed the enzyme powerpoint.