Enter Date Aim Microscope Parts YEAH Warmup Write

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Enter Date Aim: Microscope Parts … YEAH!! Warm-up: Write a hypothesis for the statement

Enter Date Aim: Microscope Parts … YEAH!! Warm-up: Write a hypothesis for the statement … Chocolate may cause pimples HW:

Engage - What keeps them living? • How do these organisms stay alive? •

Engage - What keeps them living? • How do these organisms stay alive? • How did scientists know this? • What did they use to figure this out?

Explain - Cell Theory • 1. All living things are made of cells •

Explain - Cell Theory • 1. All living things are made of cells • 2. Cells are the basic units of structure and function • 3. New cells come from existing cells

SIMPLE MICROSCOPE ▸ Hand lenses or magnifying glasses

SIMPLE MICROSCOPE ▸ Hand lenses or magnifying glasses

COMPOUND MICROSCOPE ▸ Uses two lenses ▸ Has an optical system (objective and ocular

COMPOUND MICROSCOPE ▸ Uses two lenses ▸ Has an optical system (objective and ocular lens) ▸ Has a mechanical system (moving parts) ▸ Has a light system (light source)

Compound-light Microscope • 2 sets of lenses eyepiece & objective • Specimens must be

Compound-light Microscope • 2 sets of lenses eyepiece & objective • Specimens must be thin & often dead • Image is upside-down & backwards e ə

Eye Piece Body Tube Nosepiece Low Power High Power Stage Clips Diaphragm Light Source

Eye Piece Body Tube Nosepiece Low Power High Power Stage Clips Diaphragm Light Source Arm Stage Coarse Adjustment Fine Adjustment Base

PHASE CONTRAST MICROSCOPE ▸ Allows you to view specimens without killing them. ▸ Uses

PHASE CONTRAST MICROSCOPE ▸ Allows you to view specimens without killing them. ▸ Uses depression slides

DISSECTING MICROSCOPE ▸ Has two lenses. ▸ Acts as a more user friendly magnifying

DISSECTING MICROSCOPE ▸ Has two lenses. ▸ Acts as a more user friendly magnifying glass.

ELECTRON MICROSCOPE ▸ Shoots electrons at an object to capture the image. ▸ Magnifies

ELECTRON MICROSCOPE ▸ Shoots electrons at an object to capture the image. ▸ Magnifies by 100, 000 times. ▸ Specimens must be thin.

Microscope Terms A. Magnification • How LARGE an image is B. Resolution • How

Microscope Terms A. Magnification • How LARGE an image is B. Resolution • How sharp or clear the image is • Fine adjustment aids in this

Moving Objective Under the Microscope • To center objects you need to realize that

Moving Objective Under the Microscope • To center objects you need to realize that everything is REVERSED NEED to move it to the LEFT

In which direction should the slide be moved to center the cell? 1) To

In which direction should the slide be moved to center the cell? 1) To the right & up 2) To the right and down 3) To the left and up 4) To the left and down

Paramecium Pollen Grains

Paramecium Pollen Grains

Microscope Measurement 1. Total Magnification Eyepiece x Objective Ex: 1: 10 x 10 =

Microscope Measurement 1. Total Magnification Eyepiece x Objective Ex: 1: 10 x 10 = 100 X Ex. 2: 10 x 43 = 430 X

2. Micrometer/Micron (μm) • Unit of measurement used with the microscope 1 mm =

2. Micrometer/Micron (μm) • Unit of measurement used with the microscope 1 mm = 1000 um

mm • • um Move decimal 3 places to right or Multiply by 1000

mm • • um Move decimal 3 places to right or Multiply by 1000 Ex. 1: 5 mm = ? um 5, 000 um Ex. 2: 1. 5 mm = ? um 1, 500 um

um • • mm Move decimal 3 places to left or Divide by 1000

um • • mm Move decimal 3 places to left or Divide by 1000 Ex. 1: 8 um = ? mm 0. 008 mm Ex. 2: 2. 5 um = ? mm 0. 0025 mm

About how many um are each cell? 2 mm = 2000 um 5 cells

About how many um are each cell? 2 mm = 2000 um 5 cells = 400 um/cell

What is the approximate diameter of the cell shown in the low-power field? 1)

What is the approximate diameter of the cell shown in the low-power field? 1) 100 um 2) 500 um 3) 800 um 4) 1, 000 um 1 mm = 1000 um 2 cells = 500 um/cell

3. Changing from Power to Power • Low Power Bigger field of view See

3. Changing from Power to Power • Low Power Bigger field of view See more cells, but in less detail Brighter • High Power Smaller field of view See less cells, but in more detail Darker

Low Power: 40 X

Low Power: 40 X

High Power: 400 X

High Power: 400 X

Elaborate -Prokaryote vs Eukaryote • Prok - no membrane bound nucleus • Euk -

Elaborate -Prokaryote vs Eukaryote • Prok - no membrane bound nucleus • Euk - membrane bound nucleus

PREPARING A WET MOUNT ▸ Place drop of water on a slide ▸ Add

PREPARING A WET MOUNT ▸ Place drop of water on a slide ▸ Add specimen to the water ▸ Drop coverslip at a 45 degree angle (prevents bubbles)

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