Entameoba spp BY T MOUDY BIN SALEH CLASSIFICATION

Entameoba spp. BY : T. MOUDY BIN SALEH

CLASSIFICATION OF ENTAMEOBA SPP. q Phylum : Sarcomastigophora (having flagella or pseudopodia) q Subphylum : Sarcodina (pseudopodia present) q Order : Amoebida q Genus & species : Entameoba histolytica & Entameoba coli.

INTRODUCTION • - E. histolytica - is an important pathogenic parasite of humans : Amebiasis, amebic dysentery. -The habitat of the E. histolytica trophozoites is the wall and lumen of the colon especially in the rectal regions. • - Entamoeba coli- is a non-pathogenic species of Entamoeba that frequently exists as a commensal parasite in the human gastrointestinal tract.

LIFE CYCLE OF AMOEBA HISTOLYTICA.

Habitat Entamoeba histolytica Entamoeba coli Large Intestine 10 – 30 µ 15 – 50 µ Differentiated Not differentiated 1 1 Small – central Big – eccentric Fine granules of equal size and even distribution Coarse granules of irregular size and distribution Trophozoite Size Ectoplasm & endoplasm Nucleus Karyosome peripheral chromatin

Entamoeba histolytica Entamoeba coli 15 µ 20 µ Mature cyst Size Shape Glycogen vacuoles Chromatoid bodies Round In young cyst only Often present Sometimes present (bar-like rods) (splinter-like) 4 8 Nuclei: number

Entamoeba histolytica Infective stage &Diagnostic stage Pathogenicity Mode of the infection Mature 4 nuclei cysts Pathogenic ameba Entamoeba coli Mature cysts 4 -8 nuclei Nonpathogenic ameba reside in the large intestine Ingestion of mature cysts in fecal contaminated food, water, or hands.

Entamoeba coli Mature cysts Entamoeba coli trophozoirte Entamoeba histolytica Mature cysts Entamoeba histolytica trophozoirte

Cyst of E. coli in a concentrated wet mount stained with iodine. Five nuclei are visible in this focal plane.