Enrichment analysis using DAVID By NFU Biology Algorithm

Enrichment analysis using DAVID By NFU Biology Algorithm lab

Outline • What is DAVID • DAVID Tools • Tools Demo – Functional Annotation – Gene Functional Classification – Gene ID Conversion

What is DAVID • DAVID is a Bioinformatics Database, and it is also provides many tools to analysis the gene data.

DAVID Tools provides many function about 1. Functional Annotation 2. Gene Function Classification 3. Gene ID Conversion 4. Gene Name Batch Viewer 5. NIAID Pathogen Annotation

Tools Demo • Functional Annotation – Step 1: Upload the Gene list for DAVID from Start Analysis

Tools Demo – Step 2&3: Upload and select list type • Upload list or file to DAVID • Select Identifier and Gene List

Tools Demo

Tools Demo The gene list number from users upload Upload list file name Choice what kind tools you want (ex: Functional Annotation Tool)

Tools Demo Annotation Summary Results Individual view report: Percentage (involved genes/total genes)Gene from your list Single Chart Report for ONLY this annotation category View and select annotation categories of your interests. 下一頁有建議選擇 建議三種都下載儲存

Tools Demo Annotation Summary Results • 建議如下： Select Gene-ontology (BP_1) pathways (5)

Tools Demo Functional Annotation Clustering options and stringency The overall enrichment score for the group based on the EASE score of each term members. The higher, the more enriched.

Tools Demo Functional Annotation Chart Minimum number of genes for the corresponding term. Maximum EASE Score/P-value Modified Fisher Exact P-value, EASE Score. The smaller, the more enriched. Related term search Original database/resource where the terms orient Enriched terms associated with your gene list

Tools Demo Functional Annotation Table Gene Name Annotation terms

Tools Demo Gene Functional Classification Result 點選 進入 Gene Functional classification

Tools Demo Gene Functional Classification Result Group options and stringency Group number The higher, the more enriched.

Tools Demo Gene ID Conversion 點選 進入 Gene ID Conversion

Tools Demo Gene ID Conversion 選擇想要轉換項目

Tools Demo Gene ID Conversion 使用者上傳的內容 轉換結果

Thanks For Watching PRODUCER BY NATIONAL FORMOSA UNIVERSITY BIOLOGY ALGORITHM LAB AT 2012/12