Empowering Teachers Through Modeling Protein Structure Basics LEARNING

Empowering Teachers Through Modeling Protein Structure Basics!

LEARNING OBJECTIVES Educators attending this workshop will: ØUtilize tools that support NGSS student learning outcomes of three dimensional lesson design. ØMolymods ØAmino Acid Starter Kit© ØLearn something new and interesting for your own professional development. ØHAVE FUN!

LEARNING OBJECTIVES What NGSS dimensions will be targeted? SEPs Asking questions CCCs Developing and using models Cause and effect PS 1. A: Structure and properties of matter PS 1. B: Chemical reactions Constructing explanations LS 1. A: Structure and function Patterns DCIs Scale, proportion, and quantity Structure and function LS 2. C: Ecosystem dynamics, functioning, and resilience

Protein Structure versus. . . the Mosquito The Phenomenon!

Amino Acids: Building Blocks for Proteins

Amino Acids: D and L Forms of Amino Acids

Amino Acids Exist as Zwitterions!

Amino Acid Form is Dependent on p. H!

Building a Dipeptide

The Protein Folding Problem Beware student preconceptions about protein folding! Amino Acid #1 #2

Modeling Protein Structure

Modeling the Primary Structure You will need 15 total side chains: Ø 2 cysteine side chains (green). Ø 2 acidic side chains (red). Ø 2 basic side chains (blue). Ø Your favorite 3 hydrophilic amino acids (white). Ø Your favorite 6 hydrophobic amino acids (yellow). Ø Randomly distribute them on the TOOBER. (Space them about three inches apart to get an even distribution).

Modeling Tertiary Structure Fold your protein according to basic principles of chemistry and physics: Ø In an aqueous environment, where do you expect the hydrophobic side chains to fold? Why? Ø Likewise, where would the hydrophilic side chains occur? Ø What interaction may occur between acidic and basic amino acids? Ø What interaction may occur between cysteine amino acids?

Modeling the Primary Structure Some points to ponder: 1. Why are all of the folded structures in this room different? 2. If an unlimited number of each of the 20 amino acid sidechains were available. . How many different sequences of 15 amino acids are possible? 3. It is estimated that there are 100, 000 different proteins in the body. If the average protein is about 300 amino acids long, then there are 20300 possibilities! Why are there fewer proteins in the body than possible?

Modeling Secondary Structure Beta Sheets Why are secondary structures important to protein structure/function? Alpha Helices

Modeling Quaternary Structure Hemoglobin