Dynamics of cellmediated aggregation Cristvo Dias Centro de

  • Slides: 16
Download presentation
Dynamics of cell-mediated aggregation Cristóvão Dias Centro de Física Teórica e Computacional, Universidade de

Dynamics of cell-mediated aggregation Cristóvão Dias Centro de Física Teórica e Computacional, Universidade de Lisboa, Portugal Departamento de Física, Faculdade de Ciências, Universidade de Lisboa, Portugal Encontro CIÊNCIA 2017 Lisboa, Portugal 4/Julho/2017

Morphogenesis Wound healing Genetics 156, 1671 (2000) 3 D models http: //www. biophysics. com/healingmodule.

Morphogenesis Wound healing Genetics 156, 1671 (2000) 3 D models http: //www. biophysics. com/healingmodule. php Regenerative Medicine National Geographics Andrew Pelling (http: //www. pellinglab. net)

Scaffolds 3 D printing flat substrates http: //www. wakehealth. edu/ self-assembly Soft Matter 9,

Scaffolds 3 D printing flat substrates http: //www. wakehealth. edu/ self-assembly Soft Matter 9, 2985 (2013) Biomaterials 43, 23 (2015)

Cell-mediated self-assembled scaffolds C. A. Custódio, M. T. Cerqueira, A. P. Marques, R. L.

Cell-mediated self-assembled scaffolds C. A. Custódio, M. T. Cerqueira, A. P. Marques, R. L. Reis, and J. F. Mano, Biomaterials 43, 23 (2015)

Aggregation dynamics particle-based simulations Cell adheres to the particle • n sites on the

Aggregation dynamics particle-based simulations Cell adheres to the particle • n sites on the surface (n = 6); • one site only adheres to one cell; • cell-site interaction is attractive; • colloid-colloid interaction is repulsive. cell-mediated particle-particle bond

Aggregation dynamics particle-based simulations Cell/colloid interaction Cell/Cell-colloid interaction Langevin Dynamics: Stochastic force drag term

Aggregation dynamics particle-based simulations Cell/colloid interaction Cell/Cell-colloid interaction Langevin Dynamics: Stochastic force drag term inter-particle force

Aggregation dynamics particle-based simulations (results) 512 particles total time = 100 Dcell/Dparticle = 10

Aggregation dynamics particle-based simulations (results) 512 particles total time = 100 Dcell/Dparticle = 10 10 samples Size of the largest aggregate cell/site ratio

Aggregation dynamics particle-based simulations (results) 512 particles total time = 100 Dcell/Dparticle = 10

Aggregation dynamics particle-based simulations (results) 512 particles total time = 100 Dcell/Dparticle = 10 10 samples

Aggregation dynamics mean-field approximation Free cell Cell-particle Cell adheres to the particle Particle-cell-particle cell-mediated

Aggregation dynamics mean-field approximation Free cell Cell-particle Cell adheres to the particle Particle-cell-particle cell-mediated particle-particle bond

Aggregation dynamics mean-field approximation 512 particles total time = 100 Dcell/Dparticle = 10 10

Aggregation dynamics mean-field approximation 512 particles total time = 100 Dcell/Dparticle = 10 10 samples

Aggregation dynamics mean-field approximation k 1/k 0=10 -1 5 5 k 1/k 0=10 -2

Aggregation dynamics mean-field approximation k 1/k 0=10 -1 5 5 k 1/k 0=10 -2

Aggregation dynamics lattice model (without loops)

Aggregation dynamics lattice model (without loops)

Aggregation dynamics lattice model (without loops)

Aggregation dynamics lattice model (without loops)

Aggregation dynamics lattice model (without loops)

Aggregation dynamics lattice model (without loops)

Future… Lattice model Molecular dynamics Aggregates • loops • diffusion coefficient • mechanical properties

Future… Lattice model Molecular dynamics Aggregates • loops • diffusion coefficient • mechanical properties Spatial correlations • competing timescales • aggregation dynamics • size distribution Proliferation model Cell dynamics • adhesion • chemotaxis • division • extrusion

Margarida Telo da Gama Nuno Araújo Gonçalo Antunes Universidade de Aveiro João Mano Catarina

Margarida Telo da Gama Nuno Araújo Gonçalo Antunes Universidade de Aveiro João Mano Catarina Custódio Financial support from the Portuguese Foundation for Science and Technology (FCT) under Contracts nos. EXCL/FIS-NAN/0083/2012.

Diagnosis of cellmediated responses Diagnosis of cellmediated responsesDiagnosis of cellmediated responses Diagnosis of cellmediated responses
CURATION AND AGGREGATION Tools and techniques AGGREGATION AggregationCURATION AND AGGREGATION Tools and techniques AGGREGATION Aggregation
Online Aggregation 2011 4 15 Liu Long AggregationOnline Aggregation 2011 4 15 Liu Long Aggregation
Aggregation and Secure Aggregation Why do we needAggregation and Secure Aggregation Why do we need
Rank Aggregation Rank Aggregation Settings Multiple items WebpagesRank Aggregation Rank Aggregation Settings Multiple items Webpages
Aggregation and Secure Aggregation Learning Objectives Understand whyAggregation and Secure Aggregation Learning Objectives Understand why
Lessons From Aggregation u u u 2 AggregationLessons From Aggregation u u u 2 Aggregation
Colloidal Aggregation FDSC 400 Goals Aggregation rate InteractionColloidal Aggregation FDSC 400 Goals Aggregation rate Interaction
heuristic or aggregate planning aggregation aggregation To combineheuristic or aggregate planning aggregation aggregation To combine
Aggregation Learning Objectives Understand why we need aggregationAggregation Learning Objectives Understand why we need aggregation
Rank Aggregation Rank Aggregation Settings Multiple items WebpagesRank Aggregation Rank Aggregation Settings Multiple items Webpages
Aggregation and Composition notes Chapter 4 1 AggregationAggregation and Composition notes Chapter 4 1 Aggregation
Global Aggregation Recap What is Global Aggregation ProcessGlobal Aggregation Recap What is Global Aggregation Process
Electricity Aggregation Program December 14 2020 Electricity AggregationElectricity Aggregation Program December 14 2020 Electricity Aggregation
DOIS DIAS Denisemensagensespeciaishotmail com Existem dois dias emDOIS DIAS Denisemensagensespeciaishotmail com Existem dois dias em