Due TOMORROW 1 Signed SyllabusHonor code 2 Signed
Due TOMORROW: 1) Signed Syllabus/Honor code 2) Signed Lab Safety Contract 2) Spiral Notebook (College ruled is best) – IF YOU DON’T HAVE ONE, YOU WILL BE REQUIRED TO BUY ONE FOR $1 • PUT textbook in the assigned cabinet when you bring it in- DUE BY TUESDAY • Make sure you write down the #!!!!
Anatomy of a Microscope
Objective SWU: the proper way to use a microscope SW: Identify the major parts and functions of a microscope
Microscopes are expensive instruments. They must be handled with care in order to avoid damage. • Keep the instrument under a cover when not in use. • Use only the supporting arm of the microscope to lift it. Next >
Directions Each table should have a microscope • Identify each part of the microscope on the real one and label on your handout as we go
0. Take off cover and Plug in Make sure the light turns on, or you won’t be able to see anything!
0. Wrap up cord Wrap up the cord so that none is hanging down from your lab table
Eyepiece Coarse Focus Fine Focus Objective Lenses
Let’s stop and take a look at our three objective lenses • Low power • Medium power • High power Although you will use all three, you will always work your way up to high power for your biological drawings
Comparison of the Objective lenses: LOW 40 x Can you guess what it is?
Comparison of the Objective lenses: Medium 100 x Now can you guess what it is?
Comparison of the Objective lenses: High 400 x Now?
Comparison of the Objective lenses: High. ER Human Sperm Smear (Not sure of magnification—but higher than 400 x)
Eyepiece Coarse Focus Fine Focus Body Tube Revolving Nosepiece Objective Lenses Body/Arm Inclination joint Light Base Stage Clips Stage Diaphragm/ Light Adjust
1. Find the Lowest Power Objective Lens and position it in the middle Always start your microscope lab with the lowest power objective lens in place Use the “short” lens first!!!!! Next >
2. Use the coarse focus knob to raise the objective lens Raise the microscope by turning the coarse focus knob all the way up Make it as “tall” as it can be Next >
3. Get a slide from Mrs. M. Place the Slide on the Stage Always carry the slide without putting your fingers directly on the glass Place the slide on the stage, so that the specimen is over the middle of the hole on the stage. Secure the slide in place with the stage clips. Next >
4. Lower the Objective Lens to focus Lower the objective lens using the coarse focus dial SLOWLY, until the specimen comes into focus You MAY NOT do this on when using high power! You will crush the slide and/or break the lens Try to keep both eyes open. This will help to prevent eye strain and headaches. Next >
5. Adjusting the Diaphragm (if needed) The diaphragm can be adjusted to let different amounts of light through the specimen. Trial and error Next >
6. Minor Focusing Adjustments The fine focus dial alters the height of the stage more accurately than the coarse focus dial. You may need to make minor adjustments to the objective lens using the fine focus dial to get your specimen into sharp focus. Next >
7. Change Magnification (as necessary) Change magnification, on objective lens ONE at a time. Always make sure your specimen is in focus BEFORE changing magnification Next >
Total Magnification Eyepiece x Objective lens= TOTAL Magnification If a × 10 objective lens is used, assuming the eye piece magnification is × 10, the total magnification of the image will be (× 10) × (× 10) = × 100. Next >
8. Biological Drawings Your drawings are a record of what you have seen. Make sure your pictures are as accurate as possible • Always draw AS you are looking at the specimen • If using phone or microscope imager- take the picture – make sure it is clear and then draw • Label as needed Next >
1. Always start with the low powered objective lens (short) 2. Raise coarse focus knob all the way up (tall) 3. Place slide on stage- clip 4. SLOWLY, turn coarse focus knob down until specimen is clear 5. Change magnification as necessary
THE GOLDEN RULE 6. When using High power, NEVER touch the coarse (big) focus knob
1. 2. 3. 4. 5. 6. 7. Center the low power objective (short lens) Raise coarse focus knob all the way up (tall) Remove slide Lower coarse focus all the way down (short) Unplug cord Wrap cord around base Put cover back on
• Always carry with 2 hands • Only use lens paper for cleaning • Do not force knobs • Always store covered • Keep objects clear of desk and cords (put excess cord on table)
Due TOMORROW: 1) Signed Syllabus/Honor code 2) Signed Lab Safety Contract 2) Spiral Notebook (College ruled is best) – IF YOU DON’T HAVE ONE, YOU WILL BE REQUIRED TO BUY ONE FOR $1 • PUT textbook in the assigned cabinet when you bring it in- DUE BY TUESDAY • Make sure you write down the #!!!!
Materials to Get out • • • Honor code SIGNED Lab Safety Contract SIGNED Spiral Notebook Microscope lab from yesterday PENCIL
Directions to turn in At your table, one person collects all papers. Person by Person. • Honor code on TOP • Lab Safety Contract BOTTOM – First name showing Ex: Student 1 honor code, lab safety contract Student 2 honor code, lab safety contract. .
Objective SWU: the proper way to use a microscope In pairs, SW: practice using microscopes to prepare for their future microscope labs
3 Slides Total • 2 slides your choice • Draw in pencil • High power is goal • Remember title and magnification! • 1 Crossed fiber slide
Crossed Fiber Slide • Record slide letter • Look at fibers under the microscope– Try and determine which color is on top, middle, and bottom • DO NOT DRAW A PICTURE
Available slides: • Sordaria= fungus • Allium= Onion root tip • Oedogonim= green algae • Volvox= green algae • Rbizopus w. m. = bread mold Fasciola Hepatica • Human sperm smear= sperm • Zea Mays Stem = corn stem • Allium Root Tip = onion root tip • Epithelia= simple cuboidal kidney tubules • Spirogyra Vegetative = Green algae • Tilia Stem = type of tree stem • Plasmodium Falciparum= Protozoan Parasite that causes Malaria in humans • Diatom= algae • Lilium leaf= Lilly leaf • Fasciola Hepatica- Parasitic flatworm that infects the liver. Causes “liver rot”
Microscope Lab • Move to a lab table (1 pair on each side) • Send someone to get a microscope (max 2 per lab table) • Follow microscope set-up on your notes from yesterday and BEGIN!!!! You only have today!!!
What are you looking at? • Sordaria= fungus • Allium= Onion root tip • Oedogonim= green algae • Volvox= green algae • Rbizopus w. m. = bread mold Fasciola Hepatica • Human sperm smear= sperm • Zea Mays Stem = corn stem • Allium Root Tip = onion root tip • Epithelia= simple cuboidal kidney tubules • Spirogyra Vegetative = Green algae • Tilia Stem = type of tree stem • Plasmodium Falciparum= Protozoan Parasite that causes Malaria in humans • Diatom= algae • Lilium leaf= Lilly leaf • Fasciola Hepatica- Parasitic flatworm that infects the liver. Causes “liver rot”
IPEVO Visualizer Chromebook App 1. Click the Launch button 2. Click/ Search for IPEVO Visualizer
IPEVO Visualizer Chromebook App IF NOT DOWNLOADED:
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