DNA Structure deoxyribonucleic Acid Biology Chp 9 DNA
DNA Structure (deoxyribonucleic Acid) Biology Chp. 9 DNA
DNA Structure n Double Helix – 2 twisted strands of DNA n Partially unwound double helix so you can see components
DNA composed of nucleotides 3 parts 1. Sugar (deoxyribose) 2. Phosphate group 3. Nitrogenous bases 1. 2. A–T C-G
Base Pairing Rules n A always pairs with T n C always pairs with G n A is complimentary to T and n C is complimentary to G One strand of DNA determines the other
2 2 1 3 1 Nitrogenous base 3
DNA Structure n A 5 C sugar and a phosphate group form the backbone
DNA Structure n Nitrogenous bases n. A, T, C, & G nmake up the rungs on the ladder
DNA Structure 3 D Video Clip from University of Utah (Click next 4 times)
3 methods to analyze DNA n RFLP- Restriction Fragment Length Polymorphism n Restriction enzymes cut DNA up n Not best method because we need a lot of DNA n PCR – Polymerase Chain Reaction n Polymerase – enzyme that replicates (makes more) DNA n STR – Short Tandem Repeats n Specific sequences of DNA fragments that are repeated at a particular site on a
Replication Technology n Mitochondrial DNA- from mom only n n good for hair, teeth, and bone PCRn Perhaps the most successful technology to ever emerge from LIFE SCIENCE!!! n Many Uses n Forensicsn blood, convict, exonerate false accusations n Agriculture, Vet. , Medicine, Envi. Sci. , n Microorganisms, viruses, parasites…
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