DNA Replication 5 DNA Structure Review 3 A

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DNA Replication

DNA Replication

5’ DNA Structure Review 3’ A. Sugar-Phosphate Backbone B. Nitrogen Base C. Hydrogen Bond

5’ DNA Structure Review 3’ A. Sugar-Phosphate Backbone B. Nitrogen Base C. Hydrogen Bond D. Nucleotide F. 3’ End 5’

5’ DNA Structure Review 3’ B D 5’ E C A A. Sugar-Phosphate Backbone

5’ DNA Structure Review 3’ B D 5’ E C A A. Sugar-Phosphate Backbone B. Nitrogen Base C. Hydrogen Bond D. Nucleotide E. 3’ End

DNA Helicase is: • An enzyme • Responsible for unwinding the double helix of

DNA Helicase is: • An enzyme • Responsible for unwinding the double helix of the DNA molecule • Responsible for breaking the hydrogen bonds between the nitrogen base pairs. • Creates a replication fork so that parent strands can be read and used as templates to build complementary strands of new DNA molecule.

RNA Primase is: • An enzyme • Responsible for laying an RNA primer on

RNA Primase is: • An enzyme • Responsible for laying an RNA primer on unzipped strands • Responsible for laying the foundation of the complimentary strand • Builds new strand 5’-3’

DNA Polymerase is: • An Enzyme • Responsible for reading parent Helicase strand to

DNA Polymerase is: • An Enzyme • Responsible for reading parent Helicase strand to build DNA Polymerase complimentary strand • Pairs A-T, G-C • Builds from 5’ to 3’ • Very Accurate, but not perfect

DNA Ligase is: • An enzyme • Responsible for removing RNA primers and putting

DNA Ligase is: • An enzyme • Responsible for removing RNA primers and putting in DNA bases • Fixes breaks in DNA strand • Builds 5’ to 3’

DNA Replication Review 3’ 5’ Hint: Removing RNA Primer A. B. C. D. E.

DNA Replication Review 3’ 5’ Hint: Removing RNA Primer A. B. C. D. E. F. Helicase RNA Primase DNA Polymerase DNA Ligase Leading Strand Lagging Strand & Okazaki Fragment 3’ 5’

DNA Replication Review 3’ 5’ A C F A. B. C. D. E. F.

DNA Replication Review 3’ 5’ A C F A. B. C. D. E. F. Helicase RNA Primase DNA Polymerase DNA Ligase Leading Strand Lagging Strand & Okazaki Fragment E B D 5’ 3’ Hint: Removing RNA Primer 3’ 5’

DNA Mutations A. Deletion B. Insertion C. Inversion D. Translocation

DNA Mutations A. Deletion B. Insertion C. Inversion D. Translocation

A. Interphase B. Metaphase C. Telophase & Cytokinesis D. Late Anaphase E. Anaphase F.

A. Interphase B. Metaphase C. Telophase & Cytokinesis D. Late Anaphase E. Anaphase F. Late Prophase G. Prophase

This is the “resting stage. ” Cells spend most of their time here. During

This is the “resting stage. ” Cells spend most of their time here. During this stage the cell is carrying out cell processes such as protein synthesis, photosynthesis, cellular respiration and more.

Prophase • DNA has been replicated • DNA is condensed in chromosome form •

Prophase • DNA has been replicated • DNA is condensed in chromosome form • Nuclear membrane disintegrates • Spindle fibers are visible

Metaphase • Spindle making centrosomes are at poles • Spindles are attached to chromosomes

Metaphase • Spindle making centrosomes are at poles • Spindles are attached to chromosomes • Chromosomes are lined up a central disc

Telophase • Spindles separate chromosome copies • Nuclear membrane begins to reform • Cleavage

Telophase • Spindles separate chromosome copies • Nuclear membrane begins to reform • Cleavage furrow separates cytoplasm and cell organelles