DNA Fingerprinting KEY CONCEPT DNA fingerprints identify people at the molecular level.
DNA Fingerprinting Based on: • Noncoding regions of DNA • Number of nucleotide repeats in particular parts of the genome • A person’s molecular identity • A type of restriction map using gel electrophoresis
DNA Fingerprinting A DNA fingerprint is a type of restriction map. Draw the bands for person B.
DNA Fingerprinting DNA fingerprinting & probability: • Used for identification • By investigating several parts of the genome, it’s very unlikely that two people would randomly share identical numbers of repeats (multiplying each separate probability of a match) (mother) (child 1) (child 2) (father)
DNA Fingerprinting – Individual probabilities are multiplied to find the overall probability of two DNA fingerprints randomly matching. 1 1 500 x 90 x 1 120 = 1 5, 400, 000 = 1 chance in 5. 4 million people – Several regions of DNA are used to make DNA fingerprints. – By investigating several parts of the genome, it is very unlikely that two people would randomly share identical numbers of repeats (multiplying each separate probability to find the total probability of a match)
DNA Fingerprinting Uses of DNA fingerprinting: – evidence in criminal cases – paternity tests – immigration requests – studying biodiversity – Identifying new species – tracking genetically modified crops