DNA EXTRACTION A Step By Step Process To

DNA EXTRACTION A Step By Step Process To Extract DNA

BREAKING OPEN CELLS • Separate the cells with physical means like Grinders. • Add to a solution containing salt. • Positive sodium ions in the salt attract the negatively charged phosphate groups that are located on the DNA backbone. • Detergent or a lysis buffer is used to breaks down the lipids surrounding the cell, releasing the DNA.

Separating DNA From The Proteins & Other Cellular Debris • To get a clean sample of DNA you have to purify it. • You could filter the DNA. • Also you can use protease ( protein enzyme) which degrades cellular proteins and DNAassociated proteins.

PRECIPITATING DNA WITH ALCOHOL • Finally, ice cold alcohol is added to the DNA Sample. • This is done because DNA is soluble in water but not in alcohol. • You then stir the solution. • After it is stirred you can use a pipet to remove the Precipitate.

CLEANING AND QUALITY TESTING • The DNA can be further purified now and then replace into a alkaline buffer. • Density readings by a spectrophotometer can determine the concentration and purity of the sample. • Also this can be done with gel electrophoresis to show the presence of DNA in the sample and show it’s quality.

The Extraction Of DNA Thanks! –Cam. Flann