DNA DNA Synthesis and Protein Synthesis Chapter 12

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DNA, DNA Synthesis, and Protein Synthesis Chapter 12 Notes AKA Molecular Genetics

DNA, DNA Synthesis, and Protein Synthesis Chapter 12 Notes AKA Molecular Genetics

Historical Context: Story of DNA 1900's: Scientists knew that chromosomes were responsible for traits

Historical Context: Story of DNA 1900's: Scientists knew that chromosomes were responsible for traits being inherited from parents to offspring. However, the key component of the chromosomes that actually contained the genetic information remained a mystery. Chemical analysis of chromosomes told them that the genetic material had to be either proteins or nucleic acids (DNA), but they didn't know which one was responsible for carrying the genetic information.

Griffith's Experiment (1928) In 1928, British bacteriologist Fredrick Griffith experiment What is the genetic

Griffith's Experiment (1928) In 1928, British bacteriologist Fredrick Griffith experiment What is the genetic material behind inheritance? Griffith injected two different strains of bacteria (Streptococcus pneumoniae) into mice. One strain caused infection (pathogenic/virulent) and one did not. He called the virulent strain the smooth or S strain. He called the non-virulent strain the rough or R strain.

Griffith's Experiment

Griffith's Experiment

Oswald Avery (1944) • Isolated the “transforming factor” in the S-strain • Showed it

Oswald Avery (1944) • Isolated the “transforming factor” in the S-strain • Showed it was DNA. • His results were not widely accepted…not conclusive enough. • So scientist kept looking for clearer results; Protein or DNA?

Hershey & Chase Experiment (1952) Alfred Hershey, bacteriologist Martha Chase geneticist Hershey & Chase

Hershey & Chase Experiment (1952) Alfred Hershey, bacteriologist Martha Chase geneticist Hershey & Chase Experiment Provided conclusive evidence that DNA was in fact the transforming factor.

 bacteriophage (a virus that attacks bacteria). Made of the two key components protein

bacteriophage (a virus that attacks bacteria). Made of the two key components protein and DNA

Hershey and Chase Experiment Hershey and Chase used a technique called radioactive labeling to

Hershey and Chase Experiment Hershey and Chase used a technique called radioactive labeling to trace both the protein and the DNA of the bacteriophage after it infected the bacteria (E. coli). Once the virus infected the bacteria with its genetic material, they monitored which radioactive material was inherited by the bacteria. This would identify the genetic material as proteins or DNA. Provided conclusive evidence that DNA was in fact the transforming factor.

Hershey and Chase Experiment (1952) Hypothesis 1: Hypothesis 2:

Hershey and Chase Experiment (1952) Hypothesis 1: Hypothesis 2:

Hershey and Chase (1952) to clarify. … Another graphic

Hershey and Chase (1952) to clarify. … Another graphic

The Structure and Composition of DNA Scientists were now confident that they had discovered

The Structure and Composition of DNA Scientists were now confident that they had discovered what the genetic material was, but questions remained: What is the structure of DNA? How does DNA communicate information? What they discovered is that DNA is made up of nucleotides. A nucleotide is a sugar molecule, a phosphate molecule, and a nitrogenous base. BUT…How do those nucleotides fit together in DNA?

Chargaff's Rule (1952) In the 1950 s, Erwin Chargaff discovered that in every organism

Chargaff's Rule (1952) In the 1950 s, Erwin Chargaff discovered that in every organism the amount of guanine and cytosine, and the amount of adenine and thymine was nearly equal. This is called Chargaff's rule.

DNA Structure and Composition In the DNA there are four different nitrogenous bases: Adenine

DNA Structure and Composition In the DNA there are four different nitrogenous bases: Adenine Guanine Cytosine Thymine Side note: Uracil (In RNA, replaces Thymine)

The Double Helix In 1951, Rosalind Franklin used X-rays (crystallography) to photograph DNA. The

The Double Helix In 1951, Rosalind Franklin used X-rays (crystallography) to photograph DNA. The DNA molecule was in the shape of a twisted ladder known as a double helix. Photo 51

The Double Helix

The Double Helix

Watson and Crick James Watson and Francis Crick used data from Chargaff and Franklin's

Watson and Crick James Watson and Francis Crick used data from Chargaff and Franklin's photo to build the first accurate model of DNA. Why did its structure matter? Why was everyone so anxious to find out!?

The Structure of DNA is like a twisted ladder made up of alternating strands

The Structure of DNA is like a twisted ladder made up of alternating strands of deoxyribose (sugar) and phosphate. The rails of the ladder are joined by the bases. (adenine, guanine, cytosine, and thymine)

Complementary Base Pairing Each nitrogen base pairs up with another base in what is

Complementary Base Pairing Each nitrogen base pairs up with another base in what is known as complementary base pairing. Purine bases pair with pyrimidine bases. • • Adenine and Guanine are called purines. Cytosine and Thymine are called pyrimidines. Adenine always pairs with Thymine. Guanine always pairs with Cytosine.

Purines and Pyrimidines

Purines and Pyrimidines

Complementary Base Pairing

Complementary Base Pairing

Orientation of the DNA Another important feature of the DNA structure is the orientation

Orientation of the DNA Another important feature of the DNA structure is the orientation of the DNA strands. The two strands DNA are referred to as antiparrellel, meaning they run parallel to eachother, but in opposite directions. This orientation is important to understand because it explains how DNA replicates. One end of the DNA strand is referred to as the 5' (fiveprime) end, and the other end is referred to as the 3' (threeprime) end.

DNA Orientation We will discuss the importance of this orientation later

DNA Orientation We will discuss the importance of this orientation later

How Does DNA Fit Inside A Cell? Just one strand of DNA in one

How Does DNA Fit Inside A Cell? Just one strand of DNA in one chromosome can be up to 245 million base pairs long! And remember humans have 46 chromosomes It has been estimated that if all the DNA from just one cell of a human's body was unwound, it would stretch about 6 ft long! That means the DNA in one cell is about 100, 000 times longer than the cell itself! And amazingly, it all fits into the nucleus, which only takes up about 10% of the cell's volume!

How Does DNA Fit Inside A Cell? So how does all that information fit

How Does DNA Fit Inside A Cell? So how does all that information fit into a cell? DNA coils tightly around small balls of protein called histones. Histones and phosphates from the DNA combine together to form nucleosomes. Nucleosomes combine together to form chromatin fibers, and the chromatin fibers combine together to form the chromosomes.

Chromosomes and Histones *Nucleic Acids are the largest molecules in our bodies

Chromosomes and Histones *Nucleic Acids are the largest molecules in our bodies

Watson and Crick… • "It has not escaped our notice that the specific pairing

Watson and Crick… • "It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material. "

DNA Synthesis: Semiconservative Replication The way DNA gets replicated is called semiconservative replication. In

DNA Synthesis: Semiconservative Replication The way DNA gets replicated is called semiconservative replication. In semiconservative replication, one of the strands always gets copied and the other strand is a copy from the original parent or template strand.

Stages of Replication Semiconservative Replication occurs in three stages: 1. 2. 3. unwinding base

Stages of Replication Semiconservative Replication occurs in three stages: 1. 2. 3. unwinding base pairing Joining 1 During unwinding, an enzyme called DNA helicase unwinds or unzips the DNA double helix. 2 After the strands unwind, another enzyme called DNA polymerase, adds nucleotides to the new strand in complementary base pairs. 3 Joining is more complex on the lagging strand than the leading strand… DNA Ligase joins the Okazaki fragments

Amoeba Sisters – 8 Minutes

Amoeba Sisters – 8 Minutes

Unwinding

Unwinding

Base Pairing =Adding nucleotides • DNA polymerase, adds nucleotides to the growing (new) strand

Base Pairing =Adding nucleotides • DNA polymerase, adds nucleotides to the growing (new) strand in complementary base pairs. 5’ DNA Polymerase adds complimentary nucleotides to the 3’ prime end of the growing (new) strand 3’ 5’ 3’ 5’ 3’

Leading vs. Lagging Strand Because the strands are antiparallel, one of the strands can

Leading vs. Lagging Strand Because the strands are antiparallel, one of the strands can be replicated continuously from one end to the other. This section that is replicated continuously is called the leading strand. The other strand, called the lagging strand, has to be replicated in reverse order in sections of nucleotides. These sections of nucleotides are called Okazaki

Semiconservative Replication DNA polymerases add nucleotides to the 3' end of a growing strand

Semiconservative Replication DNA polymerases add nucleotides to the 3' end of a growing strand DNA polymerases add nucleotides to the 3' end of a growing dna strand

Okazaki fragments are then glued together by Joining. The another enzyme called DNA ligase

Okazaki fragments are then glued together by Joining. The another enzyme called DNA ligase

Check Your Learning… • ANIMATION… GET IT? • CRASH COURSE: DNA Replication (Short) Whole

Check Your Learning… • ANIMATION… GET IT? • CRASH COURSE: DNA Replication (Short) Whole Video • Animation PLUS Quiz

PROTEIN SYNTHESIS • Central Dogma • Dogma is a principle or set of principles

PROTEIN SYNTHESIS • Central Dogma • Dogma is a principle or set of principles laid down by an authority as incontrovertibly true. • Central Dogma of Biology • DNA m. RNA Protein • …. proteins allow cells to function • Genotype determines proteins form/fcn which determines phenotype • T & T = Transcription and Translation

The Central Dogma DNA contains a code that is transcribed into another nucleic acid

The Central Dogma DNA contains a code that is transcribed into another nucleic acid called RNA (ribonucleic acid). RNA is the photocopy of DNA that directs synthesis of proteins. This is process is known as the Central Dogma of biology. DNA is transcribed by Messenger RNA (m. RNA). Messenger RNA carries information to the ribosomes. Ribosomes (Ribosomal RNA - r. RNA) and Transfer RNA (t. RNA) translate the code to make the proteins. This is how genes are expressed as traits. = Molecular Genetics

HOMEWORK!!! = 12. 3 Read/Notes The Central Dogma

HOMEWORK!!! = 12. 3 Read/Notes The Central Dogma

What is RNA? RNA is similar to DNA. 3 differences are: RNA contains the

What is RNA? RNA is similar to DNA. 3 differences are: RNA contains the sugar ribose instead of deoxyribose. RNA uses the nitrogen base Uracil in place of Thymine. RNA is single-stranded while DNA is doublestranded. There are three main types of RNA that play a role in protein synthesis They are: Messenger RNA (m. RNA)

Messenger RNA (m. RNA) The job or role of m. RNA is transcription. Transcription

Messenger RNA (m. RNA) The job or role of m. RNA is transcription. Transcription is the process of copying the DNA code. This is the role of messenger RNA (m. RNA). Messenger RNA enters the nucleus, a small portion of the DNA strand is copied. Then the messenger RNA leaves the nucleus after copying down a part of the code to make a protein.

Always REMEMBER the factory

Always REMEMBER the factory

Messenger RNA and Transcription After the DNA is unwound in the nucleus, an enzyme

Messenger RNA and Transcription After the DNA is unwound in the nucleus, an enzyme comes along to assist in base pairing, called RNA polymerase assists m. RNA in recording what information is found on a portion of the DNA strand. Messenger RNA transcribes the code in complementary base pairs, similar to the way DNA bases are paired during replication except when the base pair Adenine is paired, Adenine pairs with Uracil instead of Thymine. (A U)

Messenger RNA and Transcription After the m. RNA is transcribed, m. RNA can leave

Messenger RNA and Transcription After the m. RNA is transcribed, m. RNA can leave the nucleus through nuclear pores and enter into the cytoplasm to find transfer RNA (t. RNA) and ribosomal RNA (r. RNA).

Translation and Transcription Where does it happen?

Translation and Transcription Where does it happen?

Ribosomes, Transfer RNA, and Translation After a m. RNA finds a ribosome, the code

Ribosomes, Transfer RNA, and Translation After a m. RNA finds a ribosome, the code is read and translated by interpreters called transfer RNA (t. RNA). t. RNA interprets the code on the m. RNA by reading its bases in groups of three called Codons. Transfer RNA molecules each have their own Anticodon that only matches with a specific codon. Translation Animation. ( Codon – m. RNA or DNA Anticodon - t. RNA

Ribosomes, Transfer RNA, and Translation

Ribosomes, Transfer RNA, and Translation

Ribosome

Ribosome

ANIMATIONS • Complete, detailed T&T Animation • Crash Course: Transcription and Translation. • Teacher’s

ANIMATIONS • Complete, detailed T&T Animation • Crash Course: Transcription and Translation. • Teacher’s Pet: Transcription and Translation • Teaches how to use the RNA Codon Chart to find the amino acid

The Code The DNA code is read as a threebase code system. Each codon

The Code The DNA code is read as a threebase code system. Each codon matches with a specific anticodon and a specific amino acid. By joining multiple amino acids together, proteins can be assembled.

 • Only 20 amino acids.

• Only 20 amino acids.

Translation

Translation

3’ 5’ 5’ 5’ • What molecule is 1? 3’ What molecule is 2?

3’ 5’ 5’ 5’ • What molecule is 1? 3’ What molecule is 2?

Practice

Practice

BINGO! • A simple exercise to help students learn how to use a codon

BINGO! • A simple exercise to help students learn how to use a codon table to translate m. RNA into its associated amino acids. Instructions: • 1. Pass out blank bingo cards • 2. Students should fill out each of the blanks with an amino acid from the codon chart. • 3. Teacher calls out 3 bases (A, T, G, C) • 4. Students find the amino acid that is associated with the codon and mark the square (use bingo chips or pennies or other

Mutations

Mutations