DNA Chromosomes and the Nucleus DNA Structure DNA

DNA Structure § What are some of the important structural features of the DNA

DNA Structure § In addition to B-DNA what other forms exist?

DNA Structure § What is supercoiled DNA and what effect does it have? §

DNA Structure § What is the difference between topoisomerases and DNA gyrase? § How

DNA Structure § What is the DNA melting temperature?

Genomic DNA § How do we define the term “genome”? § How is genomic

Genomic DNA § What are restriction enzymes and how have they helped us to

Genomic DNA § How can restriction fragments be characterized?

Genomic DNA § How is restriction mapping carried out and what information does it

Genomic DNA § How is the chain termination method of Sanger used to determine

Genomic DNA § What is bioinformatics and proteomics? § How much variation is there

Genomic DNA § How did the work of Britten and Kohne help explain why

Genomic DNA § What is the difference between tandemly repeated DNA versus interspersed repeated

Genomic DNA § What are the possible functions of this tandemly repeated DNA? §

DNA Packaging § How is the bacterial genome structured to fit inside of the

DNA Packaging § What is the relationship between DNA and chromosomes in eukaryotic cells?

DNA Packaging § What experimental evidence supported the existence of nucleosomes?

DNA Packaging § How is the nucleosome organized? Where is the H 1 histone

DNA Packaging § How are nucleosomes packaged to form chromosomes? § What is the

DNA Packaging § How does mitocondrial and chloroplast DNA compare with nuclear DNA?

Nucleus § What do we know about nuclear structure?

Nucleus § What are the major features of the nuclear pore complex?

Nucleus § What kinds of molecules are transported across the nuclear envelope?

Nucleus § How are molecules transported across the nuclear envelope?

Nucleus § What role does the nuclear matrix serve? § What role does the

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DNA, Chromosomes and the Nucleus

DNA Structure

DNA Structure § What are some of the important structural features of the DNA double helix?

DNA Structure § In addition to B-DNA what other forms exist?

DNA Structure § What is supercoiled DNA and what effect does it have? § Can it occur in linear DNA molecules?

DNA Structure § What is the difference between topoisomerases and DNA gyrase? § How can one bring about the denaturation and renaturation of DNA?

DNA Structure

DNA Structure § What is the DNA melting temperature?

Genomic DNA § How do we define the term “genome”? § How is genomic size expressed? § Is there a relationship between genomic size and complexity of the organism or virus?

Genomic DNA

Genomic DNA § What are restriction enzymes and how have they helped us to study DNA?

Genomic DNA § How can restriction fragments be characterized?

Genomic DNA § How is restriction mapping carried out and what information does it provide?

Genomic DNA § How is the chain termination method of Sanger used to determine the sequence of a DNA molecule?

Genomic DNA § What is bioinformatics and proteomics? § How much variation is there in the human genome?

Genomic DNA § How did the work of Britten and Kohne help explain why eukaryotic genomes are so large?

Genomic DNA § What is the difference between tandemly repeated DNA versus interspersed repeated DNA?

Genomic DNA § What are the possible functions of this tandemly repeated DNA? § What about the role of this DNA fingerprinting? § in certain genetic diseases?

DNA Packaging § How is the bacterial genome structured to fit inside of the small cell? § What are bacterial plasmids?

DNA Packaging § What is the relationship between DNA and chromosomes in eukaryotic cells? § What are nucleosomes?

DNA Packaging § What experimental evidence supported the existence of nucleosomes?

DNA Packaging § How is the nucleosome organized? Where is the H 1 histone protein?

DNA Packaging § How are nucleosomes packaged to form chromosomes? § What is the DNA packing ratio?

DNA Packaging § How does mitocondrial and chloroplast DNA compare with nuclear DNA?

Nucleus § What do we know about nuclear structure?

Nucleus § What are the major features of the nuclear pore complex?

Nucleus § What kinds of molecules are transported across the nuclear envelope?

Nucleus § How are molecules transported across the nuclear envelope?

Nucleus § What role does the nuclear matrix serve? § What role does the nuclear lamina serve? § What is the function of the nucleolus?