DIGITAL SLIDE SCANNER TECHNOLOGY IN COMPARISON Why 3

DIGITAL SLIDE SCANNER TECHNOLOGY IN COMPARISON

Why 3 DHISTECH? • We develop WSI (Whole Slide Imaging) systems since 1998 • We are technology inventors dedicated to Digital Pathology • As the first European manufacturer, 3 DHISTECH is one of the market leaders in the world with more than 2000 sold systems • We are one of the TOP 3 manufacturers in the world

Scanner Comparison Pannoramic Philips Leica Hamamatsu Roche Huron Sakura Motic Zeiss

3 DHISTECH DW 2”x 3” Stnd 1”x 3” 1000 3” / 76 mm Philips Leica 300 Hamamatsu 400 2” / 52 mm Roche 360 1” / 26 mm DW Stnd 2”x 3” 1”x 3”

Scanner Comparison 3 DHISTECH® offers the most comprehensive whole slide scanner solution available today

Pannoramic Scanners MIDI II (12) MIDI CONF (12) SCAN II (150) FLASH III (250) P 1000 (1000) 4 options DW slides 4 options 2 options 4 options DW slides 8 options 2 options 8 options Fluorescent Bright Field DESK II (1)

The 3 DHISTECH digital pathology portfolio of solutions • Whole Slide Scanners

PANNORAMIC 1000 • For labs processing around 200, 000 slides per year • Unprecedented capacity and speed • Dedicated Scanner Software • Fully automated for a whole day’s scanning needs: • Speeding up the workflow • Freeing up lab resources for other tasks • “Walk away” slide scanning Key Features: • Capacity = 1000 slides • Speed = 100 slides per hour • Flexibility = 10 x-100 x magnification • Flash scanning technology • Automatic loading

Pannoramic 250 Flash III • For labs processing around 100, 000 slides per year • Award-winning speed with Flash scanning technology • Automatic switching between brightfield and fluorescent scanning based on profile content Key Features: • Capacity = 250 slides • Speed = 54 slides per hour • Brightfield and up to 9 -channel fluorescent scanning • Automatic loading

P 250 Flash III Competition

Pannoramic Scan II • For labs processing around 35, 000 slides per year • High-capacity, all-round scanner • Automatic switching between brightfield and fluorescent scanning based on profile content Key Features: • Capacity = 150 slides • Brightfield and up to 9 -channel fluorescent scanning • Automatic loading

SCAN II Competition

Pannoramic MIDI II • For low-volume slide scanning • Award-winning, versatile digital slide scanning solution • Automatic switching between brightfield and fluorescent scanning based on profile content • Compact design and a small footprint for the laboratory Key Features: • Capacity = 12 slides • Brightfield and up to 9 channel fluorescent scanning • Automatic loading

MIDI II competition

PANNORAMIC Desk II DW • Entry level slide scanner for research, education and clinical routine diagnosis • Simple and easy to use • Ideal for teleconsultation and frozen section scanning • Compact design and a small footprint for the laboratory Key Features: • Capacity = 1 slide • Single and double-width glass slide scanning • Brightfield scanning

DESK II competition

PANNORAMIC Confocal • For molecular pathology applications • Lower running costs: • Increased productivity • Automatic switching between brightfield and fluorescent scanning based on profile content • Innovative structured illumination confocal imaging Key Features: • Capacity = 11 slides • Colocalized brightfield and fluorescent scanning • Motorized slide loading • Immersion objective

Confocal competition

Scanners PANNORAMIC scanner family DESK II 1 slide Single and double width BF only Stop and go 1 camera 1 objective 30 x-110 x MIDI II 12 slides Single width only BF and FL Stop and go 1 camera 2 objectives 30 x-110 x SCAN II 150 slides Single width only BF and FL Stop and go 1 camera 2 objectives 30 x-110 x P 250 FLASH III 250 slides Single width only BF and FL FLASH 2 cameras 2 objectives 40 x, 80 x P 1000 slides Single and double width BF only FLASH 1 camera 3 objectives 40 x, 80 x

Digital / Virtual Slides • A digital whole slide is created from many small pictures • The area sensor of the camera (chip) can see one position of the stage • By moving the stage those field of views (FOV) will be recorded one by one and stitched together by the control software of the scanner • An average slice contains about 2000 such small squares on 40 x magnification

Scanner Technologies Area / Tile scanners Line / TDI scanners Camera sensor (2048 x 2048 pixel) TDI sensor (4096 pixel) x 20 or x 40 objective (≥ 64 x 4096 pixel)

Digital scanning – technologies on the market and competition There are two main technologies on the market • Line sensor technology (CCD) • TDI (Time Delay Integration) scanning • Line: 1 x 2048 pixel camera • TDI: 64 x 4096 pixel camera • works similar to a copy machine • Area scanning (CMOS) TDI (line) sensor technology: • Hamamatsu™ • Philips™ • Leica™ • Aperio™ Area sensor technology: • 3 DHISTECH™ • Zeiss™ • 3072 x 3072 pixel (9 MP) camera • Roche / Ventana™ • works similar to panorama photos • Olympus™ Zeiss, Roche, Ventana, Olympus, Hamamatsu, Philips, Leica and Aperio are trademarks or registered trademarks of their respective owners.

Advantages of Area Scanning • The stage can be stopped • Extended Focus • Z-Stack • Change the filter as many time as we want • Have any exposure time we need • Live image

Select Global Competitor Overview • Hamamatsu • Leica Biosystems • Optra. SCAN • Ventana Medical Systems (Roche) • Philips

Scanning method • Philips UFS TDI/Line Scanning • Hamamatsu S 360 TDI/Line Scanning • Leica Aperio AT 2 TDI/Line Scanning • Roche i. Scan. HT TDI/Line Scanning • Pannoramic FLASH III Area/Tile Scannnig • Pannoramic 1000 Area/Tile Scannnig • TDI vs Area has far less significance in Brightfield • Only extended focus is not possible with TDI

Example of extended focus

Scanning time Capacity Slides/hour 40 x • Philips UFS 300 30 • Hamamatsu S 360 82 • Leica Aperio AT 2 400 20 • Roche i. Scan. HT 360 20 • PANNORAMIC FLASH III 250 55 • PANNORAMIC 1000 100

Daily scanning Capacity Slides/day • Philips UFS 300 600 (2 cycle) • Hamamatsu S 360 1080 (3 cycle) • Leica Aperio AT 2 400 (1 cycle) • Roche i. Scan. HT 360 (1 cycle) • PANNORAMIC FLASH III 250 750 (3 cycle) • PANNORAMIC 1000 2000 (2 cycle)

Functions DW slide Multilayer scan • Philips UFS No No • Hamamatsu S 360 No Z-stack • Leica Aperio AT 2 2”x 3” Z-stack • Roche i. Scan. HT No Z-stack • Pannoramic FLASH III No Z-stack • Pannoramic 1000 2”x 3” Z-stack

Resolution Objectives • Philips UFS 1 Image Quality 20 x / NA 0. 75 40 x • Hamamatsu S 360 1 20 x / NA 0. 75 20 x – 40 x • Leica Aperio AT 2 1 20 x / NA 0. 75 20 x – 40 x • Roche i. Scan. HT 1 20 x / NA 0. 75 20 x – 40 x • Pannoramic FLASH III 2 40 x / NA 0. 95 20 x – 80 x • Pannoramic 1000 3 40 x / NA 1. 2 10 x – 100 x

Scanner Comparison • Aperio CS 2 BF TDI • Hamamatsu SQ BF TDI 1 • Hamamatsu S 60 BF TDI 60 • Hamamatsu S 210 BF • Motic Easy. Scan / Pro BF • Sakura Vision. Tek TDI 5 210 1/6 BF • Huron LE 120 12 BF • Huron PE 120 BF • Pannoramic DESK II BF Area • Pannoramic MIDI II BF/FL Area 12 • Pannoramic SCAN III BF/FL Area 150 Live View: Pannoramic and Sakura 2 1

Scanner Comparison • Aperio CS 2 1 obj 20 x/NA 0. 75 Z-stack • Hamamatsu SQ 1 obj 20 x/NA 0. 75 option • Hamamatsu S 60 1 obj 20 x/NA 0. 75 Z-stack • Hamamatsu S 210 1 obj 20 x/NA 0. 75 option • Motic Easy. Scan / Pro 1 obj 20 x/NA 0. 65 / 0. 75 Z-stack • Sakura Vision. Tek 1 obj 20 x/NA 0. 5 Z-stack • Huron LE 120 • Huron PE 1 obj 20 x/NA 0. 75 Z-stack 20 x/NA 0. 75 No No • Pannoramic DESK II 1 obj 40 x/NA 0. 95 Z-stack • Pannoramic MIDI II 2 obj 20 x/NA 0. 8 40 x/0. 95 Z-stack • Pannoramic SCAN III 2 obj 20 x/NA 0. 8 40 x/0. 95 Z-stack

Scanner Comparison • Aperio CS 2 4. 5 min / slide @40 x • Hamamatsu SQ 4. 5 min / slide @40 x • Hamamatsu S 60 2. 5 min / slide @40 x • Hamamatsu S 210 2. 5 min / slide • Motic Easy. Scan / Pro 6 min / slide • Sakura Vision. Tek 3 min / slide • Huron LE 120 @40 x @20 x 4 min / slide • Huron PE @40 x 10 min / slide @40 x • Pannoramic DESK II 2. 5 min / slide @40 x 5. 5 min / slide @60 x • Pannoramic MIDI II 2. 5 min / slide @40 x 5. 5 min / slide @60 x • Pannoramic SCAN III 2. 5 min / slide @40 x 5. 5 min / slide @60 x

Leica Aperio GT 450 vs 3 DHISTECH

Scanning speed and throughput Leica is claiming a 32 s scanning time per slide – but this excludes the time necessary for slide handling. They claim a throughput of 81 slides per hour, which translates into a real scanning time of 45 s per slide. On the other hand, the PANNORAMIC® 1000 has a throughput of up to 100 slides per hour and 2000 slides per day.

Scanning quality Leica uses line/TDI scanning technology, which excludes the possibility of Z-Stack or extended focus features. On the contrary, our scanners use area scanning, which makes both these features possible.

Flexibility Although labeled “Research Use Only”, the Aperio GT 450 lacks a fluorescent option. It is also unable to handle double-width slides. Our scanners provide solutions for these. The Aperio GT 450 comes with a single objective, while both our scanners have the option of two or even more objectives.

Research use only Although marketing materials for the Aperio GT 450 are hinting at clinical use, it is still for research use only. On the other hand, both our scanners can be used for diagnostic purposes in several markets.

Leica Aperio GT 450 vs 3 DHISTECH

3 DHISTECH and global competitors

Image Resolution

Scanner Comparison

Key Differentiators • QUALITY: Scanner optics • FLEXIBILITY: Multiple objective / Automated changer • EFFICIENCY: Loading capacity / Slide Management • PRODUCTIVITY: Throughput / Scanning Speed 100/hour • Scanner diversity • Scanner complexity BF & FL • Scanner image quality / resolution 10 x -100 x • Scanner camera & illumination 12 MP BF, SECTRA FL • FLASH TECHNOLOGY

Scanner Contest

Scanner Contest

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