Different Genes Protein Primary Structure Different Primary Sequences

Different Genes ~ Protein Primary Structure Different Primary Sequences ~ 3 D Structure Relative scale of conservation Genes << Primary Structure << 3 D Structure

Gene Bioinformatics Protein - Mechanism of action / biological role - Evolutionary studies, protein folding. . . - Therapeutic applications: drug/vaccine design - Biotechnological applications: diagnostics, products

1) Domain Organisation and Function Collagen Integrin I II Fibrin Heparin I FN 7 III FN 8 -10 FN 12 -14 IIIIII III Integrin III III I S, S Heparin Integrin - involved in cell adhesion, migration, matrix formation - binds to fibrin, integrin, heparin, collagens, gelatin, DNA - modular architecture consisting of type I, II and III repeats - FN 7 -10 binds integrins via the amino acid sequence RGD - FN 12 -14 binds heparin and integrin

Heparin binding Integrin binding

Integrin binding Heparin binding

GLOBAL INFORMATION FROM GENOMES MASS 2 D GE BIO-INFO SPEC

PROTEOMICS - GLOBAL INFORMATION FROM GENOMES (m. RNA EXPRESSION STUDIES) - CLASSICAL REDUCTIONIST APPROACHES

BIOCHEMICAL REDUCTIONIST APPROACHES # aa, mol. wt. , p. I, extinction coeff. Glycosylation Signal sequence Transmembrane segments Modifications (> 200) Protease cleavage sites Secondary structure prediction Function Fold, family, homologues 3 D structure Mutagenesis data Higher order complexes Expression Strategy Purification Strategy

P 16893 N C SS A-domain TM/CYT 200 aa TRM RGD Proline-rich region