Development and application of Novel Integrated Tools for

Development and application of Novel, Integrated Tools for monitoring and managing Catchments Heavy metal detection in surface water using an integrated robotic system Elisabetta De Vito-Francesco, Wolfgang Stach, Falko Ziegenbalg, Alessandro Farinelli, Jason Blum, Marc Balsells, Ruslan Alvarez, Arben Merkoçi, Thorsten Knutz, Alexander Haider, Roza Allabashi H 2 O This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 689341

Development and application of Novel, Integrated Tools for monitoring and managing Catchments Water quality awareness • Industries • Mines • Wastewater effluents Digital technologies & innovative tools for smart water monitoring Natural sources • Volcanos • Weathering soil H 2 O Anthropological sources INTCATCH vision Necessity of novel Heavy metals pollution in water bodies monitoring concepts INTEGRATED SYSTEM Robotic boat + conventional & innovative sensors H 2 O 2

Development and application of Novel, Integrated Tools for monitoring and managing Catchments Robotic boat (Uni. VR) Integrated system Heavy metal micro-fluidic detection device (ICN 2) Counter electrode - C Connection path Working electrode - C H 2 O Reference electrode – Ag/Ag. Cl Anodic stripping voltammetry • Deposition step Deposition potential is applied to the electrode, to deposit metals on working electrode (reduction) HO • Stripping step 2 Electrical current Current n. A Stripping potential is applied to the electrode, to release deposited metals to solution (oxidation) Potential V • Peak potential is specific for each metal • Peak intensity represents metal concentration 3

Development and application of Novel, Integrated Tools for monitoring and managing Catchments HM microfluidic detection device on robotic boat 1 a. HCl Electrolyte vessel 1 b. Peristaltic pump Collecting sample Collecting electrolyte 2. Mixing system 3. Bubble trap system H 2 O 4. Flow-cell Inlet/outlet solution Screen-printed sensor Settings of micro-fluidic detection device (ICN 2) Metal analysis Pb & Cu (Cd & Zn in development) Electrolyte HCl, initial concentration 0. 35 M final concentration 0. 05 M Flow rate 3 m. L/min Deposition potential -1. 0 V Stripping potential 1. 0 V H 2 O Autonomous measuring (UNIVR) • Single mode • Continous mode • Rinse command for one single measurement command for consecutive series of single measurements command to wash HM microfluidic device Measurement steps Rinsing Deposition Equilibration Stripping 180 s 20 s 10 s 4

Development and application of Novel, Integrated Tools for monitoring and managing Catchments Validation of the integrated system - Simulation of real-word scenarios H 2 O In-door trials H 2 O Water matrix Ground/Danube water Spiking levels Metals Pb: Cu (1: 4) Spike 1: 25/100 µg/L Spike 2: 50/200 µg/L Spike 3: 75/300 µg/L Spike 4: 100/400 µg/L Measuring mode continuous Out-door trials 5

Development and application of Novel, Integrated Tools for monitoring and managing Catchments Results of the in-door validation Detection of concentration changes in real-world wastewater spill-out scenario H 2 O Sensor values Pb Sensor values Cu Ref. method 1 Pb Ref. method 2 Pb Ref. method 1 Cu Ref. method 2 Cu H 2 O Performance characteristics of the integrated system (ISO 15839) 6

Development and application of Novel, Integrated Tools for monitoring and managing Catchments Results of the out-door test Autonomous navigation • Start / stop function • Vehicle velocity • Navigation path • Way points Paths shapes Homecoming function H 2 O • • H 2 O 7

Development and application of Novel, Integrated Tools for monitoring and managing Catchments Autonomous navigation Autonomous sampling & measuring Detection of concentration changes < 10 µg/L (Pb & Cu) Novel monitoring concept Real-time & continuous & in-situ H 2 O Outcomes – Integrated system H 2 O 8

Development and application of Novel, Integrated Tools for monitoring and managing Catchments H 2 O Thank you! H 2 O 9