Designed lignocellulosicbased films for tunable physicochemical and spectral

Designed lignocellulosic-based films for tunable physico-chemical and spectral properties Aguié-Béghin V, Muraille L. , Paës G, Chabbert B COST FP 1105 - 8 -9 May 2014, Coimbra, Portugal

Objective & Strategy Ø Physical , chemical , and cytochemical studies of LC deconstruction and properties Ø Determining and isolating individual features that limit LC deconstruction, and govern LC properties : role of polymer interactions • Designing Lignocellulose-based self-assemblies (films) Assemblies of plant cell walls which mimic some of the interactions, composition, physical states found in plant cell wall Nanostructured LC with variable properties COST FP 1105 - Coimbra, Portugal - V. AGUIE-BEGHIN 8 -9 May, 2014

Designing bioinspired LC assemblies Elementary building brick: Cellulose nanocrystals, nanofibrils (CNCs, CNF) (from ramie, flax, hemp fibers) Ramie CN Flax CN Aspect ratio ~ 30 -50 Hemicelluloses: feruloylated arabinoxylans, xylan, glucomannan Lignin: model lignin (DHP) and lignin from biorefinery Mw ~3000 g mol-1 Ralph et al. , 2007 COST FP 1105 - Coimbra, Portugal - V. AGUIE-BEGHIN 8 -9 May, 2014

Designing bioinspired Lignocellulose-based films Nanostructuration of bio-inspired LC assemblies: Interactions Non-covalent and covalent Modulation of polymer interactions (non covalent/ covalent linkages): - physical blending - polymerization or cross-linking of phenyl propane units via enzymatic reaction or chemical oxidation Hemicellulose (Xylan or Feruloylated Arabino. Xylan (FAX)) Non cellulosic polysaccharide- lignin (LCC) Cellulose Nanocrystals (CN) Hemicellulose - CN Hydrogen-bonds van der Waals and Hydrophobic interactions Ether bonds Bioinspired assemblies complexity COST FP 1105 - Coimbra, Portugal - V. AGUIE-BEGHIN 8 -9 May, 2014

Main results Bioinspired LC model systems Nanostructuration of LC polymers chemical properties : generate bioinspired systems with enhanced physico- Modulation of interactions (covalent, non-convalent) between polymers – Impact on physico-chemical (water sorption and spectral properties), – Impact on mechanical properties, – up-grading value of lignin in nanocomposites Relevant templates which emphasize the relative and quantitative importance of selected parameters (LC network, protein) on probes mobility: – Characterization and understanding of probe mobility in lignocelluloses – Model to predict macromolecular design of efficient lignocellulolytic enzymes COST FP 1105 - Coimbra, Portugal - V. AGUIE-BEGHIN 8 -9 May, 2014