David Boone Assistant Professor Department of Biomedical Informatics

David Boone Assistant Professor Department of Biomedical Informatics University of Pittsburgh Executive Director of UPCI Academy Director of Co. SBBI/i. BRIC dnb 14@pitt. edu; 412 -648 -7231 Major interests: 1) lnc. RNAs in Breast Cancer 2) STEM Outreach/Pipeline

lnc. RNAs in Breast Cancer • ENCODE reveals there are more lnc. RNAs than protein coding genes. • The function of most are largely unknown. • Our interest is identifying and characterizing the regulation and function of lnc. RNAs in breast cancer. Basal Normal-like Her 2 Lum. A Lum. B IGF 1 R PI 3 K -2. 0 AKT Logrank test p-value: 0. 0395 Growth Survival MAPK Proliferation % Surviving 100 m. TOR Unaltered SNHG 7 80 60 Upregulated SNHG 7 40 20 0 2. 0 0 50 100 150 200 Months Survival

Cell Number (10^4) SNHG 7 is a sno. RNA HG that regulates proliferation through a novel feedback mechanism MCF 7 Proliferation 15 si. Ctl 10 Induced genes IGF Signaling Transcripts si. Ctl si. SNHG 7 IGF 1 signaling SNHG 7 si. SNHG 7 5 Repressed genes 0 1 3 5 7 9 Days after transfection Proliferation

UPCI Academy Computer Science, Biology and Biomedical Informatics (Co. SBBI) Mission Elevate scientific research, critical thinking, and communication skills of highly motivated high school students and to provide close mentorship through formative years to create a pipeline to STEM careers. Details • 8 -week authentic research project • Mentorship • Academic preparatory experiences • Presentations • UPCI/Co. SBBI– 60/10 students per summer respectively • ~40% Underrepresented Co. SBBI Internship i. BRIC Career Outcomes • 90% of alumni in STEM fields as undergrads • Enrolled in top institutions • Authorships • Awards • National Presentations

Internship in Biomedical Research, Informatics, and Computer Science (i. BRIC) Mission Build a foundation for a career in biomedical data science for undergraduates from underrepresented backgrounds through immersive, challenging, authentic, and mentored research and training experiences in the emerging fields of big data, computer science, computational and systems biology, and biomedical informatics Co. SBBI Details • 10 -week authentic research project • Mentorship • Academic preparatory experiences • Presentations Internship i. BRIC Career

Outreach – Moonshot – School for Science and Math at Pittsburgh Form a partnership between the University of Pittsburgh and the Pittsburgh Public Schools in collaboration/consultation with the School for Science and Math at Vanderbilt to extend the first year summer research experience into a year-long endeavor. Details • 1 day a week pull-out program for 1 year • Application to Siemens and Intel after experience • Prepare students for research experience • Extend our commitment to mentorship • Communication intensive course A partnership between SSMP Co. SBBI Internship Career AND