Cutin Extract from Colocasia esculenta Gabi leaves as

Cutin Extract from Colocasia esculenta (Gabi) leaves as Blood Repellant for Fabrics

Microbial Infection in Laundry Services v. S. aureus (91. 67%), v. Pseudomonas aeruginosa 6. 82%) v. E. coli (2. 27%) v. Adenovirus v. Rotavirus, v. Hepatitis A

Out of 180 participants involved in the current study. Most of the coats were contaminated by staphylococci species and other bacteria such as Gram negative rods (Bacilli). Staphylococcus aureus (121/132) (91. 67%) was the most commonisolates. Pseudomonasaeruginosa (9/132) (6. 82%) E. coli (3/132) (2. 27%)

Results-The cuffs and pockets of the coats were the most highly contaminated areas. The level of bacterial contamination did not vary with the length of time a coat had been in use, but it increased with the degree of usage by the individual doctor. Staphylococcus aureus was isolated from a quarter of the coats examined, more commonly from those belonging to doctors in surgical specialties than medical specialties. However, Pathogenic Gram negative bacilli and other pathogenic bacteria were not isolated.

White coats are a potential source of cross infection, especialiy in surgical areas. Scrupulous hand washing should be observed before and after attending patients and it may be advisable to remove the white coat and put on a plastic apron before examining wounds. There is little microbiological reason for recommending a more frequent change of white coat than once a week, nor for excluding the wearing of white coats in non-clinical areas.

For Clinical laboratory workers to be adequately protected from exposure to infectious agents, it has been noted that three conditions must be met: appropriate garments must be worn when needed. such garments must be designed to cover the body areas potentially exposed. the fabrics used must reduce penetration of blood and body fluids to a minimum.

According to the OSHA final rule, protective equipment should prevent penetration of potentially infectious fluids “under normal conditions of use and for the duration of time that it will be used. ” Although an impervious apron or sleeve protectors might be added, the coat and latex glove combination remains the primary means of protection. (Smith et. Al , 1994)

effectiveness of six disposable (polypropylene; either spun-bond or spun-bond/meltblown/spun-bond construction) and four reusable (polyester-cotton) laboratory coats in preventing blood passage. Fabrics were tested at six time durations and five pressures J. A standard spray test used to evaluate resistance to wetting showed that reusable coats were less repellent than disposables (P <0. 05). Pressure testing showed that reusable and spun-bond coats allowed greater blood passage than the spun-bond/meltblown/spun-bond. Laboratory coats should be chosen that have sufficient resistance to blood or other body fluid passage for the task performed and for the period of time used.

Colocasia esculenta

Cutin v support biopolyester involved in waterproofing the leaves v Cutin is the main component (between 40% and 85%, w/w) of the plant cuticle. v Cutin is an amorphous and insoluble polymer v Presents a very low water sorption and permeability v High specific heat v Major physical barrier of plants to the penetration of pathogenic fungi

Objectives Extract Cutin from Colocasia esculenta leaves q Determine how much cutin we will get from a specific amount of Colocasia esculenta leaves. q Determine if cutin has enough capability to coat fabrics. q Determine the effectiveness of cutin as blood repellant. q

Methodology

Cutin Extraction Method on Tomatoes (Patent number: WO 2015028299 A 1).

Actual Experiment (Patent number: WO 2015028299 A 1).

Cutin Extraction THERMAL TREATMENT The researchers will be using the patented way of cutin extraction method (Patent number: WO 2015028299 A 1). FILTRATION DRYING PHASE ACIDIFICATION CENTRIFUGATION

Cutin Extraction THERMAL TREATMENT The researchers will be using the patented way of cutin extraction method (Patent number: WO 2015028299 A 1). FILTRATION DRYING PHASE ACIDIFICATION CENTRIFUGATION

THERMAL TREATMENT Materials: • Colocasia esculenta leaves • 3% Na. OH (0. 75 M) • Autoclave

FILTRATION Materials: • Filter paper • Erlenmeyer flask

Acidification Materials: • 6 M-12 M HCl • 5% HCI

CENTRIFUGATION Materials: • Centrifuge at 10000 -14000 rpm for 15 -20 minutes • Discard Supernatant or reintroduced for another extraction

DRYING PHASE

Fabric Application of Wax • Application on cloth • rub the cutin across fabric like bar of soap with bare hands. • Apply heat again to ensure a smoother and uniformly spread wax • Cure for 24 hours in a dry, warm place.

RESULTS AND DISCUSSION

The extracted cutin from the harvested 135. 7 g of Colocasia esculenta leaves was 4. 0 grams of cutin.

Blood repellency of Cotton – Cutin-coated and Uncoated Fabric : Cotton Observation +/ - Cutin. Coated Positive (+) Uncoated Negative (-) Fabric : Cotton Observation +/ - Cutin- Coated Positive (+) Uncoated Negative (-)

Conclusions Patent way of Cutin extraction on tomato peels (Patent number: WO 2015028299 A 1) is also applicable on Colocasia esculenta leaves. Cutin is able to coat COTTON, a type of clothing. With a direct physical application of cutin, cutin adheres to the clothing. Application of EXTRACTED cutin on cotton DID NOT remove its nature to repel water. Cutin can repel BLOOD.

RECOMMENDATIONS To coat the entire Lab coat, a greater amount of Colocasia esculenta leaves must be utilized for a greater yield in extracting cutin Modifications on Extraction (Acidification, Centrifugation and Drying) Easier and faster ways of coating cutin on fabrics Satisfactory appearance of Cutin in the fabric Antimicrobial effects of cutin on fabrics (Lost or not? ) Duration of Cutin on fabrics Effectiveness of cutin as blood repellant on different fabrics What other body fluids can cutin repel (cerebrospinal fluids, sputum, urine, seminal fluids, pleural fluids etc)