Cut lactose and xgal Inducer IPTG lactose Restriction
- Slides: 46
Cut lactose and x-gal
Inducer: IPTG, lactose
Restriction enzyme
Restriction fragment length polymorphism
Nonautonomous retrotransposons encode no protein • Alu elements contain the sequence AGCT that is recognized by the restriction enzyme Alu. I. They are present in up to a million copies in the human genome. • They can not transpose by themselves. Instead they take advantage of the retrotransposition machinery of other elements, such as LINEs.
Cut lactose and x-gal
Reporter gene
Fusion protein
Fusion protein The green fluorescent protein (GFP) is composed of 238 amino acids (26. 9 k. Da), originally isolated from the jellyfish Aequorea victoria that fluoresces green when exposed to blue light.
Figure 25. 05
GFP derivatives In 2008, Osamu Shimomura, Marty Chalfie and Roger Tsien (錢永建) won the Nobel Prize in chemistry.
重組蛋白質 在寄主(host)細胞中的表達 基因 優點 • 產量大 • 操作易 質體 染色體 4, 000 kb E. coli 缺點 • no post-translation modification • inclusion body • not secrete protein
重組蛋白質 在寄主細胞中的表達 E. coli 缺點 not secrete protein gram-positive bacteria B. subtilis proteases limited vector & promoter “signal” has not found yet
DNA library Figure 22. 23
常見的動物細胞寄主 insect cell-baculovirus mammal-retrovirus chinese hamster ovary (CHO) cells immortal cell
Growth inhibition
使用chemostat為bioreactor其產量將遠大 於batch。可是為什麼 業界使用batch的比率 遠高於chemostat? 1. Secondary products 2. Genetic instability 3. Operability and reliability 4. Market economics
- Sparq-ed
- Metabolism of drug definition
- Significance of biotransformation
- Hme inducer
- Prosthodontics a galore of opportunities
- Prolog green vs red cut
- What is consistent cut?
- Insert shot
- Cross cut vs rip saw
- Illustration of the steps in restriction digestion and pcr
- Illustrate the steps in restriction digestion and pcr
- Function of polysaccharides
- Lactose test strips
- Hemiacetal in lactose
- Blood flow restriction protocol
- Mechanism of restriction enzyme
- Function of restriction enzymes
- Restriction analysis
- Dean pomerleau
- Circular permutation with repetition
- Selective breeding definition biology
- Carte de restriction linéaire
- Symmetrical growth restriction causes
- Iugr
- Asymmetrical intrauterine growth restriction
- Intermediate accounting chapter 16
- Symmetrical growth restriction
- Symmetrical growth restriction
- Texas transportation code driving while license invalid
- Cla 1 restriction enzyme
- Restriction mapping
- Restriction enzyme mapping
- How to draw a restriction map
- Endonuclease restriction
- Restriction enzymes
- Restriction enzyme analysis of dna ap bio lab
- John gurdon experiment
- Dna fragment length
- Restriction fragment length polymorphism
- Restriction enzyme
- Restriction ends
- Restriction fragment analysis
- Restriction ends
- Restriction enzymes
- Restriction enzyme
- Diffusion restriction
- Difference between rflp and rapd