CRISPR But we are going to focus on

CRISPR……. . . But we are going to focus on Cas 9 …. . is complicated.

Who discovered CRISPR?

18 Cell 164, January 14, 2016 342 | NATURE | VOL 535 | 21 JULY 2016

Emmanuelle Charpentier Jennifer Doudna Feng Zhang

Feng Zhang’s group at MIT. Where is the boss? Who are these guys?

Emmanuelle Charpentier Jennifer Doudna Feng Zhang

“Finally, the. . history of CRISPR… underscores that scientific breakthroughs are rarely eureka moments. They are typically ensemble acts, played out over a decade or more, in which the cast becomes part of something greater than what any one of them could do alone. It’s a wonderful lesson for the general public, as well as for a young person contemplating a life in science. “ Eric Lander, MIT

Who discovered CRISPR? Rearrange letters Flip Words How was CRISPR discovered? insert

In the last paragraph of the Discussion…. . This is how science often happens…….

…and now a short pause, to consider……. . …… “nyibcviqsofinklmforpkmawerbtics” …… “ b i o in for ma tics” …… “ bioinformatics” Your challenge…. GGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCATCCAGTGCGCCCGGTTT ATCCCCGCTGATGCGGGGAACACCAGCGTCAGGCGTGAAATCTCACCGTCGTTGCCGGTTTATCCCT GCTGGCGCGGGGAACTCTCGGTTCAGGCGTTGCAAACCTGGCTACCGGGCGGTTTATCCCCGCTAAC GCGGGGAACTCGTAGTCCATCATTCCACCTATGTCTGAACTCCCGGTTTATCCCCGCTGGCGCGGGG

Do It……………… …. analyze the nucleotide sequence downstream from the iap gene in E. coli…. A paper bioinformatics activity….

The nucleotide sequence downstream from the iap gene in E. coli GGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCATCCAGTGCGCCCGGTT T ATCCCCGCTGATGCGGGGAACACCAGCGTCAGGCGTGAAATCTCACCGTCGTTGCCGGTTTATCCC T GCTGGCGCGGGGAACTCTCGGTTCAGGCGTTGCAAACCTGGCTACCGGGCGGTTTATCCCCGCTAA C GGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCATCCAGTGCGCC GCGGGGAACTCGTAGTCCATCATTCCACCTATGTCTGAACTCCCGGTTTATCCCCGCTGGCGCGGG CGGTTTATCCCCGCTGATGCGGGGAACACCAGCGTCAGGCGTGAAATCTCACCGTCGTTGC G CGGTTTATCCCTGCTGGCGCGGGGAACTCTCGGTTCAGGCGTTGCAAACCTGGCTACCGGG AACTCG CGGTTTATCCCCGCTAACGCGGGGAACTCGTAGTCCATCATTCCACCTATGTCTGAACTCC CGGTTTATCCCCGCTGGCGCGGGGAACTCG GGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCATCCAGTGCGCC CGGTTTATCCCCGCTGATGCGGGGAACACCAGCGTCAGGCGTGAAATCTCACCGTCGTTGC CGGTTTATCCCTGCTGGCGCGGGGAACTCTCGGTTCAGGCGTTGCAAACCTGGCTACCGGG CGGTTTATCCCCGCTAACGCGGGGAACTCGTAGTCCATCATTCCACCTATGTCTGAACTCC CGGTTTATCCCCGCTGGCGCGGGGAACTCG

GGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCATCCAGTGCGCC CGGTTTATCCCCGCTGATGCGGGGAACACCAGCGTCAGGCGTGAAATCTCACCGTCGTTGC CGGTTTATCCCTGCTGGCGCGGGGAACTCTCGGTTCAGGCGTTGCAAACCTGGCTACCGGG CGGTTTATCCCCGCTAACGCGGGGAACTCGTAGTCCATCATTCCACCTATGTCTGAACTCC CGGTTTATCCCCGCTGGCGCGGGGAACTCG CRISPR repeats (29) Spacer sequences (32) Clustered Regularly Interspersed Palindromic Repeats 32 29 (later shown to be foreign DNA) 32 29

What is the PAM? https: //youtu. be/i. SEEw 4 Vs_B 4 A CRISPR Whiteboard Lesson…presented by the IGI

(…think homologous recombination / crossing-over /molecular meiosis ) Non- Homologous End Joining Homology Directed Repair