CPNs Conjugated Polymer Nanoparticles Greater Brightness Sensitivity Stability

CPNs™ Conjugated Polymer Nanoparticles, Greater Brightness, Sensitivity & Stability. COVID-19 RDT TT www. streambio. co. uk Follow us @Stream_Bio

Conjugated Polymer Nanoparticles (CPNs™) Extraordinary stability - 20 months, p. H 4 -p. H 10, 120’C Fluorescent hydrophobic polymer core Excellent photostability - in cells, samples, do not fade Stable dispersion in solution - nano colloidal system Non-toxic - heavy-metal free, organic Iron Oxide or Gadolinium for Magnetic manipulation Contains iron oxide - for magnetic control options Various colours - including widely used red, yellow, green & blue as well as IR Familiar surface ligand chemistry - for attaching targeting biomolecules (Ab, oligonucleotides, Streptavidin, etc. ) 1400 x Brighter than AF 488 & 200 x brighter than Qdots in cells 1 - Greater sensitivity / Greater detection capability. Conjugated Targeting moieties bound to surface: e. g. Antibody / oligonucleotide / protein / fab fragments / azide / streptavidin Brightness = Quantum Yield x Extinction Coefficient 1. Source: The relative brightness of PEG lipid-conjugated polymer nanoparticles as fluid-phase markers in live cells, Lawrence P. Fernando et al DISCOVERBRIGHT TM

CPN™ Product range 450 400 CPN™ 420 CPN™ 475 550 500 CPN™ 510 CPN™ 550 600 CPN™ 610 650 CPN™ 680 700 NIR CPN™ 900 CPN™ 1130 CPN™ 435 Bespoke Conjugation Service Extraordinary Stability Exceptionally Bright, Magnetic. Covering the spectrum (incl NIR) Familiar surface chemistries - Streptavidin - biotin coupling - Thiol - Amine coupling - Azide - “Click” chemistry TM DISCOVERBRIGHT

The Problem Achieving sufficient sensitivity to detect infectious diseases at the earliest time point is challenging for assays in general. Currently ELISA / PCR both have issues depending on the infection And the test used to look for; - Antigen, to confirm presence of infection - Ig. M, to confirm recent infection - Ig. G, to confirm historic infection/immunity. - DNA of bacteria / virus The intense fluorescence of the CPNs offers greatly enhanced sensitivity vs current standards Improved sensitivity typically equates to earlier confirmation of infection / immunity status and enhancing the lower limited of detection; the earlier the status can be reliably confirmed, the better. CPNs are the ”Second Generation" of diagnostics offering high sensitivity & specificity with instant results Covid-19, The Need: Confirmation of immunity, confirmation of current infection, rapid diagnostic (ideally point of care) and serological mass screening TM DISCOVERBRIGHT

CPN™ Lateral Flow – A Step Change A Stable Rapid Diagnostic & Results Delivered in 15 min. When Targeting the surface proteins (covid-19) confirms presence similar to PCR via stable targeting (antibody equivalent). Greater intensity of fluorescence than europium chelates, - more sensitive detection Easier to handle and conjugate than europium Multiplex different targets on the same test with different colours. 1. Source: The relative brightness of PEG lipid-conjugated polymer nanoparticles as fluid-phase markers in live cells, Lawrence P. Fernando et al DISCOVERBRIGHT TM

CPN™ Multiplexed Lateral Flow 2 x Targets & CPN 610 / CPN 550 An ultra sensitive mass screening technique: The answer to early intervention? A simple early diagnostic: A dip test, blood / sputum / urine taken at routine consultations with GP and then is dipped with a strip looking for disease and cancer markers. Disease markers: Single / multiplex different markers that would indicate further tests needed? TM DISCOVERBRIGHT

CPN™ Multiplexed Lateral Flow Time lapse video: 10 mins 2 x targets TM DISCOVERBRIGHT

Quantification Applications ELSIA – fluorescent read out improving performance CPNs perform well in standard ELISAs giving robust fluorescent read outs Magnetic attraction allowing greater sensitivity and earlier detection of biomarkers CPNs increase sensitivity in standard ELISAs, their magnetic attraction dramatically increasing signal window and allowing key biomarkers to be detected earlier TM DISCOVERBRIGHT

Stable Targeting - Nano. Mi. Ps - Molecular Imprinted Polymers Involves the synthesis of nanoscale polymers in the presence of the epitope of the target molecule, creating an imprint in the polymer. This imprint (the binding site) is both sterically and chemically complimentary to the target. Nano. Mi. Ps are sometimes known as plastic antibodies’ Versatile and Highly Stable (Room Temperature), Alternative to Antibodies TM DISCOVERBRIGHT

CPN™ ELISA Formats Enhanced ELISA: As a result of the extreme sensitivity (brightness) of the CPNs in the form of microtitre-plate based ELISAs for high-throughput general population screening / testing: To detect antigen, to confirm presence of target To detect anti- Ig. M, to confirm recent infection To detect anti- Ig. G, to confirm historic infection/immunity. There’s a need for high throughput screening to confirm immunity which could last for a few years in order to release workers back into the economy, and then reconfirm immunity after a period of time, as well as confirmation of the presence of covid-19 infection TM DISCOVERBRIGHT

Andy Chaloner, CEO E: andy. chaloner@streambio. co. uk M: +44 (0) 7803 906648 Dr Deromott O’Callaghan, Head of Product Development E: dermott. ocallaghan@streambio. co. uk M: +44 (0) 7742 090095 Steve Self, Commercial Dir E: steve. self@streambio. co. uk M: +44 (0) 7515 128633 Follow us @Stream_Bio TM DISCOVERBRIGHT