Course description 462Biotechnology Course code BCH 462 Title
Course description (462)Biotechnology
• • Course code: BCH 462 Title: Biotechnology and Genetic engineering Credit Hours: 4 (2+2) Year/Term: 1438 -1439 H/1 St semester
Course overview This course is about understanding Biotechnology and genetic engineering by the use of the living organisms to produce goods and services for practical and industrial purposes. Purpose of course is to provide practical skills to students so that they are able to work with different biological samples and know how to maintain records, observe and analyze information in the laboratory. Student will gain the experience in techniques used to manipulate living cells and an overview of the genetic engineering methods.
Assessment Scheme • Methods of Assessment Marks (%) • 1 st continuous assessments 15 • 2 nd continuous assessments 15 • Practical assessments 30 • - Final Exam 40
Exam dates • Mid 1 • Mid 2 25 th October Wednesday 29 th November Wed (12 -1) Email: nalafaleg@ksu. edu. sa Office hours Sunday 10 -12 Monday 9 -12 Wednesday 9 -12
References • Understanding Biotechnology (2003). Aluizio Borem Santos (Author), Fabricio R. (Author), David E. Bowen • From Genes to Genome (2007) 2 nd Ed. Bollegi DM Rozycki MD Edelstein SJ ; Jeremy Dale, Malcom von Schantz. • Protein Methods. Wiley-Liss 1996. Bollegi DM Rozycki MD Edelstein SJ
Syllabus • • • • • Introduction to biotechnology and its brief history Biotechnology in research and industry Basic laboratory skills: Preparation of solutions, buffers, sterile techniques & autoclaving Diagnostic devices & equipment maintenance Environmental biotechnology Industrial enzymes and their applications Spectrophotometry and centrifugation Applications in laboratory and industry Protein isolation and purification techniques; chromatography; mass spectroscopy; western blotting Protein chemistry techniques: crystallization and analysis of protein three dimensional structure; NMR Transcriptomics & proteomics Nucleic acid isolation Purification of genomic DNA & c. DNA preparation and plasmids Restriction endonucleases & vectors DNA cloning; types and applications; development of recombinant protein; tissue cloning; animal and plant cloning Southern and Northern blotting 1 st Continuous Assessment Test
Polymerase chain reaction and applications Mutation detection methods DNA sequencing Stem cell research Introduction to cell culturing; precautions; applications Principles and practices of initiation, cultivation, maintenance and preservation of animal, plant and bacterial cultures. Preservation of continuous cell lines. • Transgenic animal and plant production; genetically modified plants and animals • • • 2 nd Continuous Test
• Introduction to techniques in immunochemistry • Radioimmunodiffusion, radioimmunoelectrophoresis, radioimmunoassay • Immunoblotting • ELISA • Bioethics: • Ethical principles in genetics; confidentiality and disclosure. • genetic services: Informed consent, genetic testing & prenatal diagnosis. • Ethical issues regarding genetic therapy and stem cell research • Final exam
- Slides: 9