CONFIDENTIAL Agilent Technologies Applications for Field Service Engineers

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CONFIDENTIAL Agilent Technologies Applications for Field Service Engineers

CONFIDENTIAL Agilent Technologies Applications for Field Service Engineers

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training MOBILE PHASE CONSIDERATIONS Flammability Ø Toxicity Ø Miscibility Ø Viscosity Ø

HPLC Applications Training MOBILE PHASE CONSIDERATIONS Flammability Ø Toxicity Ø Miscibility Ø Viscosity Ø Dissolved Salts Ø %Organic Solvent Ø p. H (Acid/Alkali) Ø Carbon Dioxide Ø Corrosion Ø CONFIDENTIAL

HPLC Applications Training Some Flammable Solvents Commonly Used Ø Ø Ø Ø Acetonitrile Methanol

HPLC Applications Training Some Flammable Solvents Commonly Used Ø Ø Ø Ø Acetonitrile Methanol Hexane, iso-Hexanes, Heptane, iso-Octane Tetrahydrofuran Toluene Acetone Ethyl Acetate Isopropanol CONFIDENTIAL

HPLC Applications Training Some Toxic Solvents Commonly Used Ø Ø Ø Ø Acetonitrile Methanol

HPLC Applications Training Some Toxic Solvents Commonly Used Ø Ø Ø Ø Acetonitrile Methanol Hexane Tetrahydrofuran Toluene Dichloromethane (Methylene Chloride) Chloroform Dimethyl Sulphoxide CONFIDENTIAL

HPLC Applications Training BURDICK & JACKSON UV Cutoff (nm) Acetonitrile UV 190 Ethylene Dichloride

HPLC Applications Training BURDICK & JACKSON UV Cutoff (nm) Acetonitrile UV 190 Ethylene Dichloride 228 Water 190 Dichloromethane 233 Hexane UV 195 Chloroform 245 Cyclohexane 200 Ethyl Acetate 256 Heptane 200 Dimethyl Acetamide 268 Isopropyl Alcohol 205 DMF 268 Dimethyl Sulfoxide 268 Methanol 205 Toluene 284 Ethyl Alcohol 210 Trifluoroacetic Acid 210 1, 2, 4 -TCB Tetrahydrofuran UV 212 1, 4 -Dioxane 215 Acetone 330 Methyl Isoamyl Ketone 330 Methyl Isobutyl Ketone 334 Ethyl Ether Iso-Octane 215 308 CONFIDENTIAL

 HPLC Applications Training HPLC SOLVENTS - UV CUTOFF Burdick & Jackson solvents are

HPLC Applications Training HPLC SOLVENTS - UV CUTOFF Burdick & Jackson solvents are arranged in order of increasing UV cutoff, the wavelength at which the solvent absorbance in a 1 cm path length cell is equal to 1 AU (absorbance unit) using water in the reference cell. Acetonitrile UV Pentane Water Hexane UV UV Cutoff (nm) 190 190 195 Cyclopentane Cyclohexane Heptane Isopropyl Alcohol 198 200 Methanol Ethyl Alcohol 2 -Methoxyethanol Methyl t-Butyl Ether n-Propyl Alcohol 205 210 Trifluoroacetic Acid Tetrahydrofuran UV n-Butyl Alcohol 1, 4 -Dioxane Ethyl Ether 210 212 210 215 205 210 215 CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training HPLC Columns ØSilica particles (1. 5~100μm) ØPolymer particles (3~50µm) CONFIDENTIAL

HPLC Applications Training HPLC Columns ØSilica particles (1. 5~100μm) ØPolymer particles (3~50µm) CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training CONFIDENTIAL

HPLC Applications Training SAMPLE PREPARATION Ø Filtration – Membrane Filters 0. 2µm, 0. 45µm

HPLC Applications Training SAMPLE PREPARATION Ø Filtration – Membrane Filters 0. 2µm, 0. 45µm (Cellulose Acetate, PVDF) Ø Centrifugation – Economical, less contamination, multiple processing CONFIDENTIAL

PS 335 Photodiode Array Detector HPLC Applications Training CONFIDENTIAL

PS 335 Photodiode Array Detector HPLC Applications Training CONFIDENTIAL

PS 335 Photodiode Array Detector HPLC Applications Training CONFIDENTIAL

PS 335 Photodiode Array Detector HPLC Applications Training CONFIDENTIAL

PS 335 Photodiode Array Detector HPLC Applications Training CONFIDENTIAL

PS 335 Photodiode Array Detector HPLC Applications Training CONFIDENTIAL

PS 335 Photodiode Array Detector HPLC Applications Training CONFIDENTIAL

PS 335 Photodiode Array Detector HPLC Applications Training CONFIDENTIAL

PS 335 Photodiode Array Detector HPLC Applications Training CONFIDENTIAL

PS 335 Photodiode Array Detector HPLC Applications Training CONFIDENTIAL

CONFIDENTIAL Agilent Technologies Applications for Field Service Engineers

CONFIDENTIAL Agilent Technologies Applications for Field Service Engineers