CONFIDENTIAL Agilent Technologies Applications for Field Service Engineers
- Slides: 30
CONFIDENTIAL Agilent Technologies Applications for Field Service Engineers
HPLC Applications Training CONFIDENTIAL
HPLC Applications Training CONFIDENTIAL
HPLC Applications Training CONFIDENTIAL
HPLC Applications Training CONFIDENTIAL
HPLC Applications Training CONFIDENTIAL
HPLC Applications Training MOBILE PHASE CONSIDERATIONS Flammability Ø Toxicity Ø Miscibility Ø Viscosity Ø Dissolved Salts Ø %Organic Solvent Ø p. H (Acid/Alkali) Ø Carbon Dioxide Ø Corrosion Ø CONFIDENTIAL
HPLC Applications Training Some Flammable Solvents Commonly Used Ø Ø Ø Ø Acetonitrile Methanol Hexane, iso-Hexanes, Heptane, iso-Octane Tetrahydrofuran Toluene Acetone Ethyl Acetate Isopropanol CONFIDENTIAL
HPLC Applications Training Some Toxic Solvents Commonly Used Ø Ø Ø Ø Acetonitrile Methanol Hexane Tetrahydrofuran Toluene Dichloromethane (Methylene Chloride) Chloroform Dimethyl Sulphoxide CONFIDENTIAL
HPLC Applications Training BURDICK & JACKSON UV Cutoff (nm) Acetonitrile UV 190 Ethylene Dichloride 228 Water 190 Dichloromethane 233 Hexane UV 195 Chloroform 245 Cyclohexane 200 Ethyl Acetate 256 Heptane 200 Dimethyl Acetamide 268 Isopropyl Alcohol 205 DMF 268 Dimethyl Sulfoxide 268 Methanol 205 Toluene 284 Ethyl Alcohol 210 Trifluoroacetic Acid 210 1, 2, 4 -TCB Tetrahydrofuran UV 212 1, 4 -Dioxane 215 Acetone 330 Methyl Isoamyl Ketone 330 Methyl Isobutyl Ketone 334 Ethyl Ether Iso-Octane 215 308 CONFIDENTIAL
HPLC Applications Training HPLC SOLVENTS - UV CUTOFF Burdick & Jackson solvents are arranged in order of increasing UV cutoff, the wavelength at which the solvent absorbance in a 1 cm path length cell is equal to 1 AU (absorbance unit) using water in the reference cell. Acetonitrile UV Pentane Water Hexane UV UV Cutoff (nm) 190 190 195 Cyclopentane Cyclohexane Heptane Isopropyl Alcohol 198 200 Methanol Ethyl Alcohol 2 -Methoxyethanol Methyl t-Butyl Ether n-Propyl Alcohol 205 210 Trifluoroacetic Acid Tetrahydrofuran UV n-Butyl Alcohol 1, 4 -Dioxane Ethyl Ether 210 212 210 215 205 210 215 CONFIDENTIAL
HPLC Applications Training CONFIDENTIAL
HPLC Applications Training CONFIDENTIAL
HPLC Applications Training CONFIDENTIAL
HPLC Applications Training CONFIDENTIAL
HPLC Applications Training CONFIDENTIAL
HPLC Applications Training CONFIDENTIAL
HPLC Applications Training CONFIDENTIAL
HPLC Applications Training CONFIDENTIAL
HPLC Applications Training CONFIDENTIAL
HPLC Applications Training HPLC Columns ØSilica particles (1. 5~100μm) ØPolymer particles (3~50µm) CONFIDENTIAL
HPLC Applications Training CONFIDENTIAL
HPLC Applications Training CONFIDENTIAL
HPLC Applications Training SAMPLE PREPARATION Ø Filtration – Membrane Filters 0. 2µm, 0. 45µm (Cellulose Acetate, PVDF) Ø Centrifugation – Economical, less contamination, multiple processing CONFIDENTIAL
PS 335 Photodiode Array Detector HPLC Applications Training CONFIDENTIAL
PS 335 Photodiode Array Detector HPLC Applications Training CONFIDENTIAL
PS 335 Photodiode Array Detector HPLC Applications Training CONFIDENTIAL
PS 335 Photodiode Array Detector HPLC Applications Training CONFIDENTIAL
PS 335 Photodiode Array Detector HPLC Applications Training CONFIDENTIAL
CONFIDENTIAL Agilent Technologies Applications for Field Service Engineers
- "agilent technologies"
- Aim lans
- Emerging database technologies
- Emerging database technologies
- Spectrum analysis basics
- Agilent oscilloscope basics
- 84115em
- Agilent u8903a
- Agilent academy
- Agilent 34401
- Kevin cowell
- Agilent
- Agilent vee
- Agilent
- "the cadence"
- Agilent 66319b
- Applications of cft
- Manufacturing and service technologies
- Gauss law in magnetism
- Individual differences in second language learning
- Field dependent vs field independent
- Electric field and magnetic field difference
- Waveguide cutoff frequency
- Database field types and field properties
- Field dependent definition
- Difference between electric field and magnetic field
- Fspos
- Typiska novell drag
- Tack för att ni lyssnade bild
- Ekologiskt fotavtryck
- Varför kallas perioden 1918-1939 för mellankrigstiden?