Comparable between Rapid One Step Immunochromatographic Assay and

Comparable between Rapid One Step Immunochromatographic Assay and ELISA in the Detection of Prostate Specific Antigen in Vaginal Specimens of Raped Women • Vichan Peonim MD , * • J Med Assoc Thai 2007; 90 (12): 2624 -9 1

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Ø��������� ELISA ��� • Horseradish peroxidase (substate ������� O-phenylene diamine ������ ) • alkaline phosphatase (substrate ����� p-nitrophenyl phosphate ������ ) ØCut off 0. 5 ng/ml 17

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������ PSA ��� rapid one step test kit • test (mouse anti-human PSA Mc antibody) • Antibody colloidal gold conjugate • ������� PSA (specific antibody- PSA-color conjugate) • ����������� 100 µl ( 3 ��� ) • cut off ��� PSA ��� 4 ng/ml 20

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• ���� 2 ������� • ����������� (gold standard) • ������ sensitivity, accuracy, specificity, positive predictive value, negative predictive value 28

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TP FP FN TN 30

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