Common Laboratory Glassware and Volumetric Glassware Prepared By

  • Slides: 47
Download presentation
Common Laboratory Glassware and Volumetric Glassware Prepared By Michigan Department of Environmental Quality Operator

Common Laboratory Glassware and Volumetric Glassware Prepared By Michigan Department of Environmental Quality Operator Training and Certification Unit

GLASSWARE Graduated Cylinder Griffin Beaker Erlenmeyer Flask Graduated Pipet

GLASSWARE Graduated Cylinder Griffin Beaker Erlenmeyer Flask Graduated Pipet

GLASSWARE Approximate Graduations Griffin Beaker

GLASSWARE Approximate Graduations Griffin Beaker

GLASSWARE Approximate Graduations Erlenmeyer Flask

GLASSWARE Approximate Graduations Erlenmeyer Flask

GLASSWARE Graduated Cylinder Used for Routine Volume Measurements of 25 m. L or Greater

GLASSWARE Graduated Cylinder Used for Routine Volume Measurements of 25 m. L or Greater

GLASSWARE Wide Tip Use When Solids in Sample Used for Routine Volume Measurements of

GLASSWARE Wide Tip Use When Solids in Sample Used for Routine Volume Measurements of 25 m. L or Less Graduated Pipet

GLASSWARE Wide Tip Use When Solids in Sample NOT VOLUMETRIC Graduated Pipet

GLASSWARE Wide Tip Use When Solids in Sample NOT VOLUMETRIC Graduated Pipet

Volumetric Glassware Required for High Accuracy and Precision Required When Preparing or Using Standard

Volumetric Glassware Required for High Accuracy and Precision Required When Preparing or Using Standard Solutions

Volumetric Glassware Accuracy and Precision Standard Solutions Buret Volumetric Flask Volumetric Transfer Pipet

Volumetric Glassware Accuracy and Precision Standard Solutions Buret Volumetric Flask Volumetric Transfer Pipet

VOLUMETRIC GLASSWARE Must Be Used Properly

VOLUMETRIC GLASSWARE Must Be Used Properly

BURET

BURET

BURET To Deliver Calibration Temperature

BURET To Deliver Calibration Temperature

Rinse With Titrant Completely Coat Inside Surface

Rinse With Titrant Completely Coat Inside Surface

Beads of Liquid Not Clean

Beads of Liquid Not Clean

Rinse Three Times Fill to Above Top Graduation

Rinse Three Times Fill to Above Top Graduation

Discharge All Air Bubbles

Discharge All Air Bubbles

Drain Until Top of Liquid Is In Graduations

Drain Until Top of Liquid Is In Graduations

Curved Surface of Liquid MENISCUS

Curved Surface of Liquid MENISCUS

MENISCUS READER

MENISCUS READER

Proper Reading X O X

Proper Reading X O X

Bottom of Meniscus to Top of Graduation

Bottom of Meniscus to Top of Graduation

Increase Ten Graduations }

Increase Ten Graduations }

9. 64 24. 16

9. 64 24. 16

Proper Grip Record Initial Reading Add Indicator

Proper Grip Record Initial Reading Add Indicator

Mix Well Near Endpoint

Mix Well Near Endpoint

Split Drops Transfer To Flask Rinse Into Reaction

Split Drops Transfer To Flask Rinse Into Reaction

At Endpoint Read Record Final Reading – Initial Reading = Volume Delivered

At Endpoint Read Record Final Reading – Initial Reading = Volume Delivered

Properly Dispose Excess Properly Clean Air Dry

Properly Dispose Excess Properly Clean Air Dry

Proper Storage

Proper Storage

Volumetric Transfer Pipets

Volumetric Transfer Pipets

One Calibration To Deliver Volumetric Transfer Pipets Calibration Temperature

One Calibration To Deliver Volumetric Transfer Pipets Calibration Temperature

Fill By Vacuum

Fill By Vacuum

Fill By Vacuum Pipet Bulb Rinse

Fill By Vacuum Pipet Bulb Rinse

Must Drain Evenly Not Clean

Must Drain Evenly Not Clean

Wipe Tip Fill Above Calibration Drain To Calibration Touch Container To Remove Drop

Wipe Tip Fill Above Calibration Drain To Calibration Touch Container To Remove Drop

Some Will Remain In Tip Allow To Drain Touch Tip To Flask

Some Will Remain In Tip Allow To Drain Touch Tip To Flask

Exception NOT Painted ETCHED Ring FOR BLOW-OUT

Exception NOT Painted ETCHED Ring FOR BLOW-OUT

Properly Clean Air Dry

Properly Clean Air Dry

Using Volumetric Pipets 1. Rinse with solution to be pipeted (Observe for even draining)

Using Volumetric Pipets 1. Rinse with solution to be pipeted (Observe for even draining) 2. Fill to above graduation 3. Wipe off outside of pipet 4. Drain to graduation ( bottom of meniscus to top of graduation) 5. Touch tip to waste vessel 6. Transfer by gravity (vertical - don’t force) 7. Touch tip to inside of receiving flask (clean pipet)

Volumetric Flasks

Volumetric Flasks

TC To Contain NOT Intended To Deliver

TC To Contain NOT Intended To Deliver

To Prepare Solutions With Accurately Known Concentration

To Prepare Solutions With Accurately Known Concentration

Fill Drop-wise To Calibration Fill Half And Mix Fill To Below Graduation

Fill Drop-wise To Calibration Fill Half And Mix Fill To Below Graduation

Invert Rapidly

Invert Rapidly

Transfer To Reagent Bottle Properly Clean Air Dry

Transfer To Reagent Bottle Properly Clean Air Dry

Volumetric Glassware Accuracy and Precision Standard Solutions Buret Volumetric Flask Volumetric Transfer Pipet

Volumetric Glassware Accuracy and Precision Standard Solutions Buret Volumetric Flask Volumetric Transfer Pipet

Common Laboratory Glassware and Volumetric Glassware Prepared By Michigan Department of Environmental Quality Operator

Common Laboratory Glassware and Volumetric Glassware Prepared By Michigan Department of Environmental Quality Operator Training and Certification Unit