Clinical laboratory diagnostics of hemoblasosis Leukemia definition l

Clinical laboratory diagnostics of hemoblasosis

Leukemia definition l. Leukemia – it is tumor arising from bloodforming cells and primary lesion bone marrow

Classification of leukemias Acute leukemias l myeloblastic l lymphoblastic l Chronical leukemias l myeloid l lynphoid l

Acute myeloblastic leukemias l Undifferentiated l Myeloblastic without maturing l Myeloblastic with maturing l Promyelocytic l Myelomonoblastic l Monoblastic l Erythroblastic l Megacaryoblastic

Histochemical signs of differentiation of acute leukemias l 1. Reaction for peroxidase l 2. Reaction for acidity phosphatase l 3. Reaction for uncpecific esterases l 4. Reaction for lipides l 5. Reaction for compoud carbohydrates

Reaction of myeloblastes and other of neutrophil cells for peroxidase

Reaction of monoblastes for naphtylacetateestherase α-

Reaction of myeloblastes and monoblastes for acidity phosphatase

Acute lymphoblastic leukemia - blood

Acute В-lymphoblastosis – bone marrow Big vacuolized lymphoblastes

Acute lymphoblastic leukemia – bone marrrow

Acute lymphoblastic leukemia – bone marrrow. Initial stage.

Acute lymphoblastosis – bone marrow. Total lymphoblastic metaplasia

Chronical myeloid leukemias Myelocytic l Monocytic l Eosinophilic l Erythroblastic l Megacaryocytic l

Chronical myelocytic leukemia – blood. Chronical phase

Chronical myelocytic leukemia – blood.

Chronical myelocytic leukemia – blood Stage of blastic crisis

Chronical lymphoid leukemias В-lymphocytic l Т- lymphocytic (1, 5 %) l Haircell l

Chronical В-cell lymphocytic leukemia - blood

Chronical lymphocytic leukemia – blood. Gumprecht’s bodies.

Chronical lymphocytic leukemia – bone marrow. Total lymphoid metaplasia.

Chronical haircell leukemia - blood

Haircell leukemia - blood. Reaction for acidity phosphatase

Chronical monocytic leukemia - blood

Mechanisms of cellular oncogenes activating l. Point mutations l. Chromosomal abnormalities l. Virual transduction l. Insertion mutagenesis l. Genes amplification

Clinical manifestation of leukemia l. Metaplastic anemia l. Thrombocytopenia l. Hemorrhagic syndrome l. Secondary immunedeficiency l. Decrease of resistance

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