Clinical Features of Food Allergy Ig E Essential

Clinical Features of Food Allergy 대부분 알레르기 반응은 특이적 Ig. E를 매게로 함: • 두드러기 • 혈관 부종 • 기관지 천식 • 설사/ 구토 • 아토피성 피부염 • 과민증 Essential participants Dendritic cells T cells B cells Mast cells Ig. E Fce. RI allergen (Ara h 2) (2 Ig. E epitopes) mast cells histamine & leukotrienes

Food Allergy Facts § True food allergy is an Ig. E-mediated disease § Occurrence: § 2% of adults § 6 -8% of young children § Eight foods or food groups account for ~90% of allergies: Milk Eggs Fish Crustacea Wheat Peanuts Soybeans Tree nuts Disease management by avoidance (elimination diets)

Principles of Allergy Assessment § Avoid transfer of known allergens § Assume genes from allergenic sources encode an allergen until proven otherwise § Assess the allergenic potential of all introduced proteins § Where allergens are identified, consider alternative sources or technical strategies to reduce risk, or discontinue development

Decision Process § Compare sequence with known allergens § if similar, do clinical tests with appropriate patients § if not, proceed § Perform digestion in pepsin § if stable, consult with agency for further testing § if rapidly digested, proceed § Submit data to regulatory agencies

Bioinformatics Databases Allergens - Database of known allergens (752+ AA sequences, >170 proteins) - relevant matches > 7 contiguous identical AA - or significant overall similarity (“E - score”) - to identify potentially cross-reactive proteins Toxins - Database of known protein toxins - currently contains 2700 AA Sequences - overall matches & specific structures - to identify potentially toxic proteins Total - Total sequences: 3. 1 x 105 known genes Evaluate results, determine if specific tests are needed.

Digestive Fate Tool § Employs USP simulated gastro-intestinal digestion models § Broadly applicable p. H 1. 2 § food safety Pepsin § nutritional sciences § Extensive validation § relevance to food allergy § Quick results on test § less than two weeks