CHROMATOGRAPHY AP BIO LAB 4 A Image by

CHROMATOGRAPHY AP BIO LAB #4 A Image by Riedell http: //www. valemount. com/joel/lightoptics/images/colour 6. jpg

PLANT PIGMENTS. http: //members. aol. com/diaspore/vhouse/biology/projects/photosynthesis/woida/meaning_of_light. html#question_1

CHROMATOGRAPHY USE PENCIL not pen, to mark starting line !!!!! Image by Riedell

ADD PLANT PIGMENTS Image from Biology Place: http: //www. phschool. com/science/biology_place/labbench/lab 4/deposit. html

Image by Riedell PLACE IN JAR IMPORTANT: Make sure tip of paper is touching solvent Make sure pigment line is ABOVE solvent Put lid on jar !!!!

Important Don’t let solvent front run off end of paper ! http: //www. phschool. com/science/biology_place/labbench/lab 4/pigsep. html

Marker Chromatography Image by Riedell

Distance pigment migrated Rf = Distance solvent front migrated Image by Riedell

PLANT PIGMENTS Carotene is orange yellow Xanthophyll is lemon yellow Chlorophyll a is blue green Chlorophyll b is yellow green. Image by Riedell

Factors that affect movement of molecules during chromatography Solubility 1. ______of molecule in solvent used (better it dissolves… farther it will move) SOLVENT used: Chlorophyll: 9: 1 Petroleum ether : acetone Markers: water

Factors that affect movement of molecules during chromatography Size 2. _____of molecule (bigger molecules move slower) http: //elvis. phys. lsu. edu/svoboda/post/figs/Sumo. Wrestler. BMP

Factors that affect movement of molecules during chromatography 3. ____________ used Attraction to media (EX: Attraction of molecules to paper) Lots of different kinds of chromatography http: //courses. cm. utexas. edu/jrobertus/ch 339 k/overheads-1/ch 5_mol-seive. jpg http: //www. cofc. edu/~kinard/Applets/Gas. Chromatograph. FID. gif

Factors that affect movement of molecules during chromatography Time 4. _____allowed to run Longer time = More separation http: //www 1. istockphoto. com/file_thumbview_approve/581259/2/istockphoto_581259_big_time. jpg

CHLOROPHYLL FLUORESCENCE Image from Biology AP Edition by Campbell & Reece published by Pearson Benjamin Cummings