Chromatographic Techniques Day 1 9 00 Introduction 9

Chromatographic Techniques Day 1 9: 00 Introduction 9: 15 Lecture: Gel filtration 10: 00 Coffee Break 10: 15 Introduction practical work and AKTA System demo 11: 00 Exp 1: Gel Filtration-Desalting mode 11: 30 Lecture: Ion Exchange 12: 30 Lunch 1: 30 Discussion results Exp. 1/ Introduction Exp. 2 2: 00 Practical: Exp 2: Ion exchange- Media/p. H scouting 2: 30 Lecture: Analytical Techniques 3: 30 Coffee Break 4: 00 Discussion of results Exp. 2/ Intro Exp. 3 4: 30 Practical: Exp. 3: Ion exchange- binding capacity 6: 00 End of day 1 1/ GE / 12/21/2021

Chromatographic Techniques Day 2 8: 30 Discussion of results Exp. 3/ Intro Exp. 4 8: 45 Practical: Exp. 4: IEC scouting for step 2 9: 30 Lecture: Purification Strategies 10: 15 Coffee break 10: 30 Analysis: Discussions results Exp. 4 11: 00 Lecture: Hydrophobic interactions & Reversed phase chromatography 12: 00 Lunch 1: 00 Intro Exp. 5 1: 15 Practical: Exp. 5: HIC-scouting 2: 00 Lecture: Affinity chromatography 2: 45 Analysis: Discussions results Exp. 5/ Intro Exp. 6 3: 15 Practical: Exp. 6: IEC/ HIC contd. (step 2) 3: 30 Coffee break 3: 45 Lecture: Optimization (general) 5: 00 End of day 2/ GE / 12/21/2021

Chromatographic Techniques Day 3 8: 30 Lecture: Optimization (different techniques 9: 30 Discussion of results Exp. 6/ Intro Exp. 7 9: 45 Practical: Exp. 7: Affinity chromatography 10: 15 Coffee break 10: 30 Lecture: Scale up and fine tuning 11: 30 Discussion of results Exp. 7 12: 00 Lunch 1: 00 Lecture: Process hygiene 1: 45 Lecture: Regulation: A brief introduction 2: 15 Coffee Break 2: 30 Lecture: Column Packing (Video) 3: 00 Discussion of results and Q&A 4: 00 End of Course 3/ GE / 12/21/2021