Chiba i GEM 2007 Marimo What the hell
Chiba i. GEM 2007 Marimo
What the hell is Marimo? • Marimo (Japanese: 毬藻 marimo), also known as Cladophora ball, Lake ball, or Moss Balls in English, is a species of filamentous green algae (Chlorophyta) found in a number of lakes in the northern hemisphere. • blabla…
To make a bacterial Marimo 1 3 まりも完成 2
Our System 1. Gather bacteria – Quorum Sensing 2. Stick bacteria together – flagella: fli. C gene 3. Control size (advanced) – Tune Quorum Sensing
Detailed Design: Sender Cell • Constitutively express flagella • Produce AHL
Detailed Design: Reciever Cell • Lux. P > flagella • no AHL > aii. A ? ?
Experiments & Results Quorum sensing, Fli. C-his gene
Experiments 1. Quorum Sensing – – modify? for a lab-class 自由溶液 size-tunable 2. Flagella (fli. C) engeneering
Quorum Sensing in a liquid… • To make the AHL conc. gradation – Sender(=AHL producer) Problem • Senderを固める(拡散させない) • 必要量のAHLを出すSenderの数 – AHL拡散 Problem • solvent’s viscosity • AHL溜る -> 分解する(aii. A)
Size Control • Super Sender Cell – Produce more AHL • Super Reciever Cell – Sensitive
To Make a Marimo 1. Check the flagella adsorption w/Quorum sensing 2. 2 -dimensional test @ plate 3. 3 -dimensional (marimo) 4. Size control test
Controling Marimo Size
What we learned
Acknowledgements
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