Chapter 6 Microbial Growth Physical Requirements Chemical Requirements

Chapter 6: Microbial Growth Physical Requirements Chemical Requirements Growth of Bacterial Cultures Culture Media Measurement of Growth $100 $100 $200 $200 $300 $300 $400 $400 $500 $500 © 2013 Pearson Education, Inc. FINAL ROUND

Topic 1: Physical Requirements $100 Question The optimum p. H for most bacteria is a. b. c. d. 1. 5. 7. 9. ANSWER © 2013 Pearson Education, Inc. BACK TO GAME

Topic 1: Physical Requirements $100 Answer The optimum p. H for most bacteria is a. b. c. d. 1. 5. 7. 9. © 2013 Pearson Education, Inc. BACK TO GAME

Topic 1: Physical Requirements $200 Question A psychrophile grows best at a. b. c. d. 37°C. 10°C. 25°C. 15°C. ANSWER © 2013 Pearson Education, Inc. BACK TO GAME

Topic 1: Physical Requirements $200 Answer A psychrophile grows best at a. b. c. d. 37°C. 10°C. 25°C. 15°C. © 2013 Pearson Education, Inc. BACK TO GAME

Topic 1: Physical Requirements $300 Question Organisms that require high salt concentrations for growth are called a. b. c. d. halophiles. extreme halophiles. facultative halophiles. microhalophiles. ANSWER © 2013 Pearson Education, Inc. BACK TO GAME

Topic 1: Physical Requirements $300 Answer Organisms that require high salt concentrations for growth are called a. b. c. d. halophiles. extreme halophiles. facultative halophiles. microhalophiles. © 2013 Pearson Education, Inc. BACK TO GAME

Topic 1: Physical Requirements $400 Question What is the energy source utilized by producers in the hydrothermal vents on the ocean floor? a. b. c. d. light hydrogen sulfide organic molecules carbon dioxide ANSWER © 2013 Pearson Education, Inc. BACK TO GAME

Topic 1: Physical Requirements $400 Answer What is the energy source utilized by producers in the hydrothermal vents on the ocean floor? a. b. c. d. light hydrogen sulfide organic molecules carbon dioxide © 2013 Pearson Education, Inc. BACK TO GAME

Topic 1: Physical Requirements $500 Question In food preservation, salts and sugars are used because they create a a. b. c. d. lower p. H. hypertonic environment. hypotonic environment. lower osmotic pressure. ANSWER © 2013 Pearson Education, Inc. BACK TO GAME

Topic 1: Physical Requirements $500 Answer In food preservation, salts and sugars are used because they create a a. b. c. d. lower p. H. hypertonic environment. hypotonic environment. lower osmotic pressure. © 2013 Pearson Education, Inc. BACK TO GAME

Topic 2: Chemical Requirements $100 Question Chemoautotrophs and photoautotrophs derive their carbon from a. b. c. d. carbon dioxide. proteins. lipids. carbohydrates. ANSWER © 2013 Pearson Education, Inc. BACK TO GAME

Topic 2: Chemical Requirements $100 Answer Chemoautotrophs and photoautotrophs derive their carbon from a. b. c. d. carbon dioxide. proteins. lipids. carbohydrates. © 2013 Pearson Education, Inc. BACK TO GAME

Topic 2: Chemical Requirements $200 Question Some microorganisms use gaseous nitrogen directly from the atmosphere in a process called a. b. c. d. chemosynthesis. photosynthesis. nitrogen fixation. nitrogen synthesis. ANSWER © 2013 Pearson Education, Inc. BACK TO GAME

Topic 2: Chemical Requirements $200 Answer Some microorganisms use gaseous nitrogen directly from the atmosphere in a process called a. b. c. d. chemosynthesis. photosynthesis. nitrogen fixation. nitrogen synthesis. © 2013 Pearson Education, Inc. BACK TO GAME

Topic 2: Chemical Requirements $300 Question An organism that uses oxygen but can grow without oxygen is called a(n) a. b. c. d. obligate aerobe. facultative aerobe. aerotolerant anaerobe. ANSWER © 2013 Pearson Education, Inc. BACK TO GAME

Topic 2: Chemical Requirements $300 Answer An organism that uses oxygen but can grow without oxygen is called a(n) a. b. c. d. obligate aerobe. facultative aerobe. aerotolerant anaerobe. © 2013 Pearson Education, Inc. BACK TO GAME

Topic 2: Chemical Requirements $400 Question Which organism is killed by atmospheric oxygen? a. b. c. d. aerobe obligate anaerobe aerotolerant anaerobe ANSWER © 2013 Pearson Education, Inc. BACK TO GAME

Topic 2: Chemical Requirements $400 Answer Which organism is killed by atmospheric oxygen? a. b. c. d. aerobe obligate anaerobe aerotolerant anaerobe © 2013 Pearson Education, Inc. BACK TO GAME

Topic 2: Chemical Requirements $500 Question Biofilms reside in a matrix made up primarily of a. b. c. d. lipids. hydrogel. proteins. polysaccharides. ANSWER © 2013 Pearson Education, Inc. BACK TO GAME

Topic 2: Chemical Requirements $500 Answer Biofilms reside in a matrix made up primarily of a. b. c. d. lipids. hydrogel. proteins. polysaccharides. © 2013 Pearson Education, Inc. BACK TO GAME

Topic 3: Growth of Bacterial Cultures $100 Question Generation time can best be defined as the a. b. c. d. length of time it takes for lag phase. duration of log phase. length of time it takes for a cell to divide. minimum rate of doubling. ANSWER © 2013 Pearson Education, Inc. BACK TO GAME

Topic 3: Growth of Bacterial Cultures $100 Answer Generation time can best be defined as the a. b. c. d. length of time it takes for lag phase. duration of log phase. length of time it takes for a cell to divide. minimum rate of doubling. © 2013 Pearson Education, Inc. BACK TO GAME

Topic 3: Growth of Bacterial Cultures $200 Question How many cells would be present after four generations if we started with three cells? a. b. c. d. 12 24 48 96 ANSWER © 2013 Pearson Education, Inc. BACK TO GAME

Topic 3: Growth of Bacterial Cultures $200 Answer How many cells would be present after four generations if we started with three cells? a. b. c. d. 12 24 48 96 © 2013 Pearson Education, Inc. BACK TO GAME

Topic 3: Growth of Bacterial Cultures $300 Question In which growth phase does the number of cells change very little because cells do NOT immediately reproduce in a new medium? a. b. c. d. stationary phase log phase death phase lag phase ANSWER © 2013 Pearson Education, Inc. BACK TO GAME

Topic 3: Growth of Bacterial Cultures $300 Answer In which growth phase does the number of cells change very little because cells do NOT immediately reproduce in a new medium? a. b. c. d. stationary phase log phase death phase lag phase © 2013 Pearson Education, Inc. BACK TO GAME

Topic 3: Growth of Bacterial Cultures $400 Question Which of the following is NOT a possible reason why exponential growth stops? a. b. c. d. accumulation of waste products exhaustion of nutrients harmful changes in p. H All of these are possible reasons. ANSWER © 2013 Pearson Education, Inc. BACK TO GAME

Topic 3: Growth of Bacterial Cultures $400 Answer Which of the following is NOT a possible reason why exponential growth stops? a. b. c. d. accumulation of waste products exhaustion of nutrients harmful changes in p. H All of these are possible reasons. © 2013 Pearson Education, Inc. BACK TO GAME

Topic 3: Growth of Bacterial Cultures $500 Question In the stationary phase, a. the number of microbial deaths exceeds the number of new cells formed. b. the number of microbial deaths is equal to the number of new cells formed. c. new cells are no longer formed because of adverse environmental conditions. d. cellular reproduction is most active. ANSWER © 2013 Pearson Education, Inc. BACK TO GAME

Topic 3: Growth of Bacterial Cultures $500 Answer In the stationary phase, a. the number of microbial deaths exceeds the number of new cells formed. b. the number of microbial deaths is equal to the number of new cells formed. c. new cells are no longer formed because of adverse environmental conditions. d. cellular reproduction is most active. © 2013 Pearson Education, Inc. BACK TO GAME

Topic 4: Culture Media $100 Question Which type of medium suppresses the growth of unwanted bacteria and distinguishes colonies of the desired organisms from other organisms? a. b. c. d. selective media complex media selective and differential media enrichment media © 2013 Pearson Education, Inc. ANSWER BACK TO GAME

Topic 4: Culture Media $100 Answer Which type of medium suppresses the growth of unwanted bacteria and distinguishes colonies of the desired organisms from other organisms? a. b. c. d. selective media complex media selective and differential media enrichment media © 2013 Pearson Education, Inc. BACK TO GAME

Topic 4: Culture Media $200 Question The microbes that grow and multiply in or on a culture medium are referred to as a(n) a. b. c. d. culture. inoculum. specimen. medium. ANSWER © 2013 Pearson Education, Inc. BACK TO GAME

Topic 4: Culture Media $200 Answer The microbes that grow and multiply in or on a culture medium are referred to as a(n) a. b. c. d. culture. inoculum. specimen. medium. © 2013 Pearson Education, Inc. BACK TO GAME

Topic 4: Culture Media $300 Question What can be added to a medium when it is desirable to grow bacteria on a solid medium? a. b. c. d. glucose magnesium sulfate potassium phosphate agar ANSWER © 2013 Pearson Education, Inc. BACK TO GAME

Topic 4: Culture Media $300 Answer What can be added to a medium when it is desirable to grow bacteria on a solid medium? a. b. c. d. glucose magnesium sulfate potassium phosphate agar © 2013 Pearson Education, Inc. BACK TO GAME

Topic 4: Culture Media $400 Question A culture medium consisting of agar, human blood, and beef heart is a _____ medium. a. b. c. d. complex selective differential chemically defined ANSWER © 2013 Pearson Education, Inc. BACK TO GAME

Topic 4: Culture Media $400 Answer A culture medium consisting of agar, human blood, and beef heart is a _____ medium. a. b. c. d. complex selective differential chemically defined © 2013 Pearson Education, Inc. BACK TO GAME

Topic 4: Culture Media $500 Question The source of nutrients in nutrient agar is a. b. c. d. agar. peptone and sodium chloride. peptone and beef extract. nutrient. ANSWER © 2013 Pearson Education, Inc. BACK TO GAME

Topic 4: Culture Media $500 Answer The source of nutrients in nutrient agar is a. b. c. d. agar. peptone and sodium chloride. peptone and beef extract. nutrient. © 2013 Pearson Education, Inc. BACK TO GAME

Topic 5: Measurement of Growth $100 Question A spectrophotometer can be used to measure a. b. c. d. metabolic activity. turbidity. dry weight. pathogenicity. ANSWER © 2013 Pearson Education, Inc. BACK TO GAME

Topic 5: Measurement of Growth $100 Answer A spectrophotometer can be used to measure a. b. c. d. metabolic activity. turbidity. dry weight. pathogenicity. © 2013 Pearson Education, Inc. BACK TO GAME

Topic 5: Measurement of Growth $200 Question Because of time considerations, which method is usually used to count the number of bacteria in milk? a. b. c. d. the direct microscope count method the pour plate method the spread plate method the most probable number method ANSWER © 2013 Pearson Education, Inc. BACK TO GAME

Topic 5: Measurement of Growth $200 Answer Because of time considerations, which method is usually used to count the number of bacteria in milk? a. b. c. d. the direct microscope count method the pour plate method the spread plate method the most probable number method © 2013 Pearson Education, Inc. BACK TO GAME

Topic 5: Measurement of Growth $300 Question Which method is utilized to ensure that plates containing 25 to 250 colonies are counted? a. b. c. d. the most probable number method serial dilution filtration spread plate method ANSWER © 2013 Pearson Education, Inc. BACK TO GAME

Topic 5: Measurement of Growth $300 Answer Which method is utilized to ensure that plates containing 25 to 250 colonies are counted? a. b. c. d. the most probable number method serial dilution filtration spread plate method © 2013 Pearson Education, Inc. BACK TO GAME

Topic 5: Measurement of Growth $400 Question Which is the best technique to use to measure the growth of filamentous organisms? a. b. c. d. most probable number method direct microscopic count turbidity dry weight ANSWER © 2013 Pearson Education, Inc. BACK TO GAME

Topic 5: Measurement of Growth $400 Answer Which is the best technique to use to measure the growth of filamentous organisms? a. b. c. d. most probable number method direct microscopic count turbidity dry weight © 2013 Pearson Education, Inc. BACK TO GAME

Topic 5: Measurement of Growth $500 Question Which technique is frequently used to detect and enumerate coliform bacteria? a. b. c. d. turbidity spread plate method filtration pour plate method ANSWER © 2013 Pearson Education, Inc. BACK TO GAME

Topic 5: Measurement of Growth $500 Answer Which technique is frequently used to detect and enumerate coliform bacteria? a. b. c. d. turbidity spread plate method filtration pour plate method © 2013 Pearson Education, Inc. BACK TO GAME

FINAL ROUND Question An experiment began with 5 cells and ended with 160 cells. How many generations did the cells go through? a. b. c. d. 32 6 4 5 ANSWER © 2013 Pearson Education, Inc. BACK TO GAME

FINAL ROUND Answer An experiment began with 5 cells and ended with 160 cells. How many generations did the cells go through? a. b. c. d. 32 6 4 5 © 2013 Pearson Education, Inc. BACK TO GAME