Chapter 10 Molecular Biology of the Gene Molecular

Chapter 10 Molecular Biology of the Gene: (Molecular Genetics) Power. Point Lectures for Biology: Concepts and Connections, Fifth Edition – Campbell, Reece, Taylor, and Simon Lectures by Chris Romero Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings

1. Nucleic Acid Structure DNA and RNA are polymers of nucleotides • DNA is a nucleic acid – Made of long chains of nucleotide monomers Sugar-phosphate backbone Phosphate group A C Nitrogenous base Sugar DNA nucleotide A C Nitrogenous base (A, G, C, or T) Phosphate group O H 3 C O T T O P O CH 2 O– G HC O T T C Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings N N C H O HC CH H Sugar (deoxyribose) DNA nucleotide DNA polynucleotide C Thymine (T) O C H G H C

• DNA has four kinds of nitrogenous bases – A, T, C, and G H O H 3 C H C C C H H N N C H O C C N H H N N C H O H H Thymine (T) Cytosine (C) Pyrimidines Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings C N C N H O N N C H C N H H C C C N N C H Adenine (A) Guanine (G) Purines H N H H

• RNA is also a nucleic acid – But has a slightly different sugar – And has U instead of T Nitrogenous base (A, G, C, or U) O Phosphate group H O O P O H CH 2 C C N H O Uracil (U) O– O C H H C C H O OH Sugar (ribose) Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings Key Hydrogen atom Carbon atom Nitrogen atom Oxygen atom Phosphorus atom

DNA is a double-stranded helix • James Watson and Francis Crick – Worked out the three-dimensional structure of DNA, based on work by Rosalind Franklin Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings

• The structure of DNA – Consists of two polynucleotide strands wrapped around

• Hydrogen bonds between bases – Hold the strands together • Each base pairs with a complementary partner – A with T, and G with C G C T A A Base pair T C G C C G A T T O O P –O O H 2 C G T O OH P O O H 2 C –O A O O –O P O H 2 C A A T A Hydrogen bond OH O O A T A OH G A Ribbon model O CH 2 O O– O P O O CH 2 O O– P O O O CH 2 O O– P HO O G C T CH 2 O O– P O O C G O O C T T Partial chemical structure Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings Computer model

2. DNA REPLICATION DNA replication depends on specific base pairing • DNA replication – Starts with the separation of DNA strands • Then enzymes use each strand as a template – To assemble new nucleotides into complementary strands A T A T A T C G C G C G C A T A T A Parental molecule of DNA C A Nucleotides Both parental strands serve as templates Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings Two identical daughter molecules of DNA

• DNA replication is a complex process, which occurs during “S” Phase of the eukaryotic cell cycle. – Due in part to the fact that some of the helical DNA molecule must untwist G C A T G C Replication Fork C C C T T A T A A G G G A G T A C Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings G C G A T C T G G C C C A T G T A T A A T

DNA replication: A closer look • DNA replication – Begins at specific sites on the double helix Origin of replication Parental strand Daughter strand Bubble Two daughter DNA molecules Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings

At the end of each replication bubble is a replication fork, a Y-shaped region where new DNA strands are elongating Helicases are enzymes that untwist the double helix at the replication forks Single-strand binding protein binds to and stabilizes single-stranded DNA until it can be used as a template Topoisomerase corrects “overwinding” ahead of replication forks by breaking, swiveling, and rejoining DNA strands Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings

• Each strand of the double helix – Is oriented in the opposite direction 5 end P 4 3 P 3 end 5 2 1 A C 2 T 1 5 HO 3 4 P G P P G C P P T OH 3 end Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings A P 5 end

• Using the enzyme DNA polymerase – The cell synthesizes one daughter strand as a continuous piece • The other strand is synthesized as a series of short pieces – Which are then connected by the enzyme DNA ligase DNA polymerase molecule 5 3 Parental DNA 3 5 Daughter strand synthesized continuously 3 5 5 3 DNA ligase Overall direction of replication Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings Daughter strand synthesized in pieces

THE FLOW OF GENETIC INFORMATION FROM DNA TO RNA TO PROTEIN • The DNA genotype is expressed as proteins, which provide the molecular basis for phenotypic traits • The information constituting an organism’s genotype – Is carried in its sequence of its DNA bases • A particular gene, a linear sequence of many nucleotides – Specifies a polypeptide Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings

3. Gene Transcription • The DNA of the gene is transcribed into RNA – Which is translated into the polypeptide DNA Transcription RNA Translation Protein Figure 10. 6 A Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings

Genetic information written in codons is translated into amino acid sequences • The “words” of the DNA “language” – Are triplets of bases called codons • The codons in a gene – Specify the amino acid sequence of a polypeptide Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings

DNA molecule Gene 1 Gene 2 Gene 3 DNA strand A A A C C G G C A A Transcription RNA Translation U U U G G C C G U U Codon Polypeptide Amino acid Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings

The genetic code is the Rosetta stone of life • Nearly all organisms –

• An exercise in translating the genetic code Strand to be transcribed T A C T T C A A T C A T G A A G T T A G U A G DNA Transcription A U G A A G U U U RNA Start condon Stop condon Translation Polypeptide Met Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings Lys Phe

Transcription produces genetic messages in the form of RNA • A close-up view of transcription RNA nucleotides RNA polymerase A A T C C A T A G G T Direction of transcription Newly made RNA Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings A T A G U C A U G U T C G T T C C A A C C Template Strand of DNA

• In the nucleus, the DNA helix unzips – And RNA nucleotides line up along one strand of the DNA, following the base pairing rules • As the single-stranded messenger RNA (m. RNA) peels away from the gene – The DNA strands rejoin Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings

• Transcription of a gene RNA polymerase DNA of gene Promoter DNA Terminator DNA 1 Initiation 2 Elongation 3 Termination Completed RNA Figure 10. 9 B Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings Area shown In Figure 10. 9 A Growing RNA polymerase

Eukaryotic RNA is processed before leaving the nucleus • Noncoding segments called introns are spliced out – And a cap and a tail are added to the ends Exon Intron Exon DNA Cap RNA transcript with cap and tail Transcription Addition of cap and tail Introns removed Tail Exons spliced together m. RNA Coding sequence Nucleus Cytoplasm Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings

4. Translation: Gene to Protein Transfer RNA molecules serve as interpreters during translation • Translation – Takes place in the cytoplasm Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings

• A ribosome attaches to the m. RNA – And translates its message into a specific polypeptide aided by transfer RNAs (t. RNAs) Amino acid attachment site Hydrogen bond RNA polynucleotide chain Anticodon Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings

• Each t. RNA molecule – Is a folded molecule bearing a base triplet called an anticodon on one end • A specific amino acid – Is attached to the other end Amino acid attachment site Anticodon Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings

Ribosomes build polypeptides • A ribosome consists of two subunits – Each made up of proteins and a kind of RNA called ribosomal RNA t. RNA molecules Growing polypeptide Large subunit m. RNA Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings Small subunit

• The subunits of a ribosome – Hold the t. RNA and m. RNA close together during translation t. RNA-binding sites Large subunit Next amino acid to be added to polypeptide Growing polypeptide m. RNAbinding site Small subunit Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings t. RNA m. RNA Codons

An initiation codon marks the start of an m. RNA message Start of genetic

• m. RNA, a specific t. RNA, and the ribosome subunits – Assemble during initiation Met Large ribosomal subunit Initiator t. RNA P site UA C A U G U A C A U G Start codon 1 m. RNA A site Small ribosomal subunit Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings 2

Elongation adds amino acids to the polypeptide chain until a stop codon terminates translation • Once initiation is complete – Amino acids are added one by one to the first amino acid Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings

• Each addition of an amino acid – Occurs in a three-step elongation process Amino acid Polypeptide P site A site m. RNA Codons Anticodon 1 Codon recognition m. RNA movement Stop codon 2 Peptide bond formation New Peptide bond 3 Translocation Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings

• The m. RNA moves a codon at a time – And a t. RNA with a complementary anticodon pairs with each codon, adding its amino acid to the peptide chain Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings

• Elongation continues – Until a stop codon reaches the ribosome’s A site,

Review: The flow of genetic information in the cell is DNA RNA protein • The sequence of codons in DNA, via the sequence of codons – Spells out the primary structure of a polypeptide Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings

• Summary of transcription and translation DNA Transcription 1 m. RNA is transcribed from a DNA template. m. RNA polymerase Amino acid Translation 2 Each amino acid attaches to its proper t. RNA with the help of a specific enzyme and ATP. Enzyme ATP t. RNA Anticodon Large ribosomal subunit Initiator t. RNA 3 Initiation of polypeptide synthesis The m. RNA, the first t. RNA, and the ribosomal subunits come together. Start Codon m. RNA Small ribosomal subunit New peptide bond forming Growing polypeptide 4 Elongation A succession of t. RNAs add their amino acids to the polypeptide chain as the m. RNA is moved through the ribosome, one codon at a time. Codons m. RNA Polypeptide 5 Termination The ribosome recognizes a stop codon. The poly-peptide is terminated and released. Stop codon Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings

Mutations can change the meaning of genes • Mutations are changes in the DNA base sequence – Caused by errors in DNA replication or recombination, or by mutagens Normal hemoglobin DNA C T Mutant hemoglobin DNA T m. RNA A T G U A C m. RNA G A A Normal hemoglobin Glu Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings Sickle-cell hemoglobin Val

1. Point Mutations a. A gene mutation is a change that takes place within a single gene. Point mutations are mutations that involved one base pair and may include. . . substitutions, insertions or deletions of nucleotides. The sentence below contains all three-letter words but has experienced a mutation. Can you find the mutation? THE IGR EDC ATA TET HEB IGB ADR AT What kind of mutation did you find? Now what does the sentence say? Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings

• Substituting, inserting, or deleting nucleotides alters a gene – With varying effects on the organism Normal gene m. RNA A U G A A G U U U G G C A Met Protein Lys Phe Gly Ala Base substitution A U G A A G U U U A G C A Met Lys Phe Ser Ala U Missing Base deletion A U G A A G U U G G C A U Met Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings Lys Leu Ala His

MICROBIAL GENETICS Viral DNA may become part of the host chromosome • Viruses –

• When phage DNA enters a lytic cycle inside a bacterium – It is replicated, transcribed, and translated • The new viral DNA and protein molecules – Then assemble into new phages, which burst from the host cell Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings

• In the lysogenic cycle – Phage DNA inserts into the host chromosome and is passed on to generations of daughter cells • Much later – It may initiate phage production Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings

• Phage reproductive cycles Phage 1 Attaches to cell Bacterial chromosome Phage DNA Cell lyses, releasing phages Phage injects DNA 7 2 Many cell divisions 4 Lytic cycle Lysogenic cycle Phages assemble Phage DNA circularizes 3 5 OR New phage DNA and proteins are synthesized Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings Lysogenic bacterium reproduces normally, replicating the prophage at each cell division Prophage 6 Phage DNA inserts into the bacterial chromosome by recombination

CONNECTION Many viruses cause disease in animals • Many viruses cause disease – When they invade animal or plant cells • Many, such as flu viruses – Have RNA, rather than DNA, as their Membranous genetic material envelope RNA Protein coat Glycoprotein spike Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings

• Some animal viruses – Steal a bit of host cell membrane as a protective envelope – Can remain latent in the host’s body for long periods Glycoprotein spike VIRUS Protein coat Envelope Viral RNA (genome) Plasma membrane 1 of host cell 2 Viral RNA (genome) 3 Entry Uncoating RNA synthesis by viral enzyme 4 Protein m. RNA synthesis New viral proteins 5 RNA synthesis (other strand) Template 6 Assembly Exit 7 Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings New viral genome

CONNECTION Plant viruses are serious agricultural pests • Most plant viruses – Have RNA genomes – Enter their hosts via wounds in the plant’s outer layers Protein RNA Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings

CONNECTION Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings Colorized TEM 370,

The AIDS virus makes DNA on an RNA template • HIV, the AIDS virus – Is a retrovirus Envelope Glycoprotein Protein coat RNA (two identical strands) Reverse transcriptase Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings

• Inside a cell, HIV uses its RNA as a template for making DNA – To insert into a host chromosome Viral RNA CYTOPLASM 1 RNA strand NUCLEUS Chromosomal DNA 2 Doublestranded DNA 3 Provirus DNA 4 Viral RNA and proteins 5 RNA 6 Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings

Bacteria can transfer DNA in three ways • Bacteria can transfer genes from cell to cell by one of three processes – Transformation, transduction, or conjugation DNA enters cell Fragment of DNA from another bacterial cell Bacterial chromosome (DNA) Mating bridge Phage Fragment of DNA from another bacterial cell (former phage host) Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings Sex pili Donor cell (“male”) Recipient cell (“female”)

• Once new DNA gets into a bacterial cell – Part of it may then integrate into the recipient’s chromosome Donated DNA Recipient cell’s chromosome Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings Crossovers Degraded DNA Recombinant chromosome

Bacterial plasmids can serve as carriers for gene transfer • Plasmids – Are small circular DNA molecules separate from the bacterial chromosome Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings

• Plasmids can serve as carriers – For the transfer of genes F factor (plasmid) F factor (integrated) Male (donor) cell Origin of F replication Bacterial chromosome F factor starts replication and transfer of chromosome Male (donor) cell Bacterial chromosome F factor starts replication and transfer Only part of the chromosome transfers Plasmid completes transfer and circularizes Plasmids Recombination can occur Cell now male Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings Colorized TEM 2, 000 Recipient cell