Center for Homogeneous DNA Analysis Software April 21

Second Year Advances • Background removal • Melting curve calculators • Automatic clustering and

Background removal • Superior to baseline method • Enables correct classification of unlabeled probe

Melting curve calculators • More informative than just Tm calculator • Enhances design of

Automatic clustering and classification algorithms • Used in HLA identity testing, mutation scanning, and

WWW availability • • • Center for Homogeneous DNA analysis Homepage: www. dna. utah.

The coming year • Determine a complete set of Tm Parameters • superior background

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Center for Homogeneous DNA Analysis - Software April 21, 2005

Second Year Advances • Background removal • Melting curve calculators • Automatic clustering and classification

Background removal • Superior to baseline method • Enables correct classification of unlabeled probe melting curves • Makes HLA matching, repeat typing and sequencing more accurate • Improves determination of thermodynamic parameters, and therefore all PCR design and analysis tools • In use by ARUP Labs

Melting curve calculators • More informative than just Tm calculator • Enhances design of full genotyping studies • Improves determination of thermodynamic parameters

Automatic clustering and classification algorithms • Used in HLA identity testing, mutation scanning, and genotyping

WWW availability • • • Center for Homogeneous DNA analysis Homepage: www. dna. utah. edu DNAWizards beta site by remote panels URL: DNAWizards. path. utah. edu Small amplicon primer design: SNPWizard Melting curve calculators: Melt 1. 0, Het 1. 0

The coming year • Determine a complete set of Tm Parameters • superior background removal • realistic experimental conditions (dyes, salt) • mismatched duplexes (synthetic) • Genome wide primer promiscuity prevention • Further develop user-friendly suite of programs • Commercial launch of DNAWizards. com