Cellular Division copyright cmassengale 1 Cell Division All
- Slides: 102
Cellular Division copyright cmassengale 1
Cell Division üAll cells are derived from pre- existing cells üNew cells are produced for growth and to replace damaged or old cells üDiffers in prokaryotes (bacteria) and eukaryotes (protists, fungi, plants, & animals) copyright cmassengale 2
Keeping Cells Identical The instructions for making cell parts are encoded in the DNA, so each new cell must get a complete set of the DNA molecules copyright cmassengale 3
DNA Replication üDNA must be copied or replicated before cell division üEach new cell will then have identical copy the DNA Original DNA strand Two new, identical DNA strands an of copyright cmassengale 4
Identical Daughter Cells Two identical daughter cells Parent Cell copyright cmassengale 5
Chromosomes copyright cmassengale 6
Prokaryotic Chromosome ü The DNA of prokaryotes (bacteria) is one, circular chromosome attached to the inside of the cell membrane copyright cmassengale 7
Eukaryotic Chromosomes üAll eukaryotic information in cells store genetic chromosomes ü Most eukaryotes have between 10 and 50 chromosomes in their body cells ü Human body cells have 46 chromosomes or 23 identical pairs copyright cmassengale 8
Eukaryotic Chromosomes üEach chromosome is composed of a single, tightly coiled DNA molecule üChromosomes can’t be seen when cells aren’t dividing and are called chromatin copyright cmassengale 9
Compacting DNA into Chromosomes üDNA is tightly coiled around proteins called histones copyright cmassengale 10
Chromosomes in Dividing Cells üDuplicated chromosomes are called chromatids & are held together by the centromere Called Sister Chromatids copyright cmassengale 11
Karyotype ü A picture of the chromosomes from a human cell arranged in pairs by size ü First 22 pairs are called autosomes ü Last pair are the sex chromosomes ü XX female or XY male copyright cmassengale 12
Boy or Girl? The Y Chromosome Decides Y - Chromosome X - Chromosome copyright cmassengale 13
Cell Reproduction copyright cmassengale 14
Types of Cell Reproduction üAsexual reproduction involves a single cell dividing to make 2 new, identical daughter cells üMitosis & binary fission are examples of asexual reproduction üSexual reproduction involves two cells (egg & sperm) joining to make a new cell (zygote) that is NOT identical to the original cells üMeiosis is an example copyright cmassengale 15
Cell Division in Prokaryotes copyright cmassengale 16
Cell Division in Prokaryotes ü Prokaryotes such as bacteria divide into 2 Parent cell identical cells by the process of binary fission Chromosome ü Single chromosome replicates makes a copy of itself ü Cell wall forms Cell splits between the chromosomes dividing the cell 2 identical daughter cells copyright cmassengale 17
Prokaryotic Cell Undergoing Binary Fission copyright cmassengale 18
Animation of Binary Fission copyright cmassengale 19
The Cell Cycle copyright cmassengale 20
Five Phases of the Cell Cycle üG 1 - primary growth phase üS – synthesis; DNA replicated üG 2 - secondary growth phase collectively these 3 stages are called interphase üM - mitosis üC - cytokinesis copyright cmassengale 21
Cell Cycle copyright cmassengale 22
Interphase - G 1 Stage ü 1 st growth stage after cell division üCells mature by making more cytoplasm & organelles üCell carries on its normal metabolic activities copyright cmassengale 23
Interphase – S Stage üSynthesis stage üDNA is copied or replicated Two identical copies of DNA Original DNA copyright cmassengale 24
Interphase – G 2 Stage ü 2 nd Growth Stage üOccurs after DNA has been copied üAll cell structures needed for division are made (e. g. centrioles) üBoth organelles & proteins are synthesized copyright cmassengale 25
What’s Happening in Interphase? What the cell looks like Animal Cell What’s occurring copyright cmassengale 26
Sketch the Cell Cycle DNA Copied Cells prepare for Division Cells Mature Daughter Cells Cell Divides into Identical cells copyright cmassengale 27
Mitosis copyright cmassengale 28
Mitosis üDivision of the nucleus üAlso called karyokinesis üOnly occurs in eukaryotes üHas four stages üDoesn’t occur in some cells such as brain cells copyright cmassengale 29
Four Mitotic Stages üProphase üMetaphase üAnaphase üTelophase copyright cmassengale 30
Early Prophase üChromatin in nucleus condenses to form visible chromosomes üMitotic spindle forms from fibers in cytoskeleton or centrioles (animal) Cytoplasm Nucleolus Nuclear Membrane Chromosomes copyright cmassengale 31
Late Prophase üNuclear membrane & nucleolus are broken down üChromosomes continue condensing & are clearly visible üSpindle fibers called kinetochores attach to the centromere of each chromosome üSpindle finishes forming between the poles of the cell copyright cmassengale 32
Late Prophase Chromosomes Nucleus & Nucleolus have disintegrated copyright cmassengale 33
Spindle Fiber attached to Chromosome Kinetochore Fiber Chromosome copyright cmassengale 34
Review of Prophase What the cell looks like What’s happening copyright cmassengale 35
Spindle Fibers üThe mitotic spindle form from the microtubules in plants and centrioles in animal cells üPolar fibers extend from one pole of the cell to the opposite pole üKinetochore fibers extend from the pole to the centromere of the chromosome to which they attach üAsters are short fibers radiating from centrioles copyright cmassengale 36
Sketch The Spindle copyright cmassengale 37
Metaphase üChromosomes, attached to the kinetochore fibers, move to the center of the cell üChromosomes are now lined up at the equator Equator of Cell Pole of the Cell copyright cmassengale 38
Metaphase Asters at the poles Spindle Fibers copyright cmassengale Chromosomes lined at the Equator 39
Metaphase Aster Chromosomes at Equator copyright cmassengale 40
Review of Metaphase What the cell looks like What’s occurring copyright cmassengale 41
Anaphase üOccurs rapidly üSister chromatids are pulled apart to opposite poles of the cell by kinetochore fibers copyright cmassengale 42
Anaphase Sister Chromatids being separated copyright cmassengale 43
Anaphase Review What the cell looks like What’s occurring copyright cmassengale 44
Telophase üSister chromatids at opposite poles üSpindle disassembles üNuclear envelope forms around each set of sister chromatids üNucleolus reappears üCYTOKINESIS occurs üChromosomes reappear as chromatin copyright cmassengale 45
Comparison of Anaphase & Telophase copyright cmassengale 46
Cytokinesis üMeans division of the cytoplasm üDivision of cell into two, identical halves called daughter cells üIn plant cells, cell plate forms at the equator to divide cell üIn animal cells, cleavage furrow forms to split cell copyright cmassengale 47
Cytokinesis Cleavage furrow in animal cell Cell plate in plant cell copyright cmassengale 48
Mitotic Stages copyright cmassengale 49
Daughter Cells of Mitosis üHave the same number of chromosomes as each other and as the parent cell from which they were formed üIdentical to each other, but smaller than parent cell üMust grow in size to become mature cells (G 1 of Interphase) copyright cmassengale 50
Identical Daughter Cells What is the 2 n or diploid number? 2 Chromosome number the same, but cells smaller than parent cell copyright cmassengale 51
Review of Mitosis copyright cmassengale 52
Draw & Learn these Stages copyright cmassengale 53
Draw & Learn these Stages copyright cmassengale 54
Name the Mitotic Stages: Interphase Name this? Prophase Telophase Name this? Metaphase Anaphase copyright cmassengale 55
Eukaryotic Cell Division ü Used for growth and repair ü Produce two new cells identical to the original cell ü Cells are diploid (2 n) Prophase Metaphase Chromosomes during Metaphase of mitosis Anaphase Telophase Cytokinesis copyright cmassengale 56
Mitosis Animation Name each stage as you see it occur? copyright cmassengale 57
Mitosis in Onion Root Tips Do you see any stages of mitosis? copyright cmassengale 58
Test Yourself over Mitosis copyright cmassengale 59
Mitosis Quiz copyright cmassengale 60
Mitosis Quiz copyright cmassengale 61
Name the Stages of Mitosis: Early Anaphase Early prophase Metaphase Interphase Late Prophase Late telophase, Mid-Prophase Advanced cytokinesis copyright cmassengale Early Telophase, Begin cytokinesis Late Anaphase 62
Identify the Stages ? Early, Middle, & Late Prophase ? ? Metaphase Late Prophase Late Anaphase ? ? Telophase copyright cmassengale ? ? Telophase & Cytokinesis 63
Locate the Four Mitotic Stages in Plants Anaphase Telophase Metaphase Prophase copyright cmassengale 64
Uncontrolled Mitosis ü If mitosis is not controlled, unlimited cell division occurs causing cancerous tumors ü Oncogenes are special proteins that increase the chance that a normal cell develops into a tumor cell Cancer cells copyright cmassengale 65
Meiosis Formation of Gametes (Eggs & Sperm) copyright cmassengale 66
Facts About Meiosis üPreceded by interphase which includes chromosome replication üTwo meiotic divisions --- Meiosis I and Meiosis II üCalled Reduction- division üOriginal cell is diploid (2 n) üFour daughter cells produced that are monoploid (1 n) copyright cmassengale 67
Facts About Meiosis üDaughter cells contain half the number of chromosomes as the original cell üProduces gametes (eggs & sperm) üOccurs in the testes in males (Spermatogenesis) üOccurs in the ovaries in females (Oogenesis) copyright cmassengale 68
More Meiosis Facts ü Start with 46 double stranded chromosomes (2 n) üAfter 1 division - 23 double stranded chromosomes (n) üAfter 2 nd division - 23 single stranded chromosomes (n) ü Occurs in our germ cells that produce gametes copyright cmassengale 69
Why Do we Need Meiosis? üIt is the fundamental basis of sexual reproduction üTwo haploid (1 n) gametes are brought together through fertilization to form a diploid (2 n) zygote copyright cmassengale 70
Fertilization – “Putting it all together” 2 n = 6 1 n =3 copyright cmassengale 71
Replication of Chromosomes ü Replication is the process of duplicating a chromosome ü Occurs prior to division ü Replicated copies are called sister chromatids ü Held together at centromere Occurs in Interphase copyright cmassengale 72
A Replicated Chromosome Gene X Sister Chromatids Homologs (same genes, different alleles) (same genes, same alleles) Homologs separate in meiosis I and therefore different alleles separate. copyright cmassengale 73
Meiosis Forms Haploid Gametes ü Meiosis must reduce the chromosome number by half ü Fertilization then restores the 2 n number from mom from dad child too much! meiosis reduces genetic content The right number! copyright cmassengale 74
Meiosis: Two Part Cell Division Sister chromatids separate Homologs separate Meiosis II Diploid copyright cmassengale Haploid 75
Meiosis I: Reduction Division Spindle fibers Nucleus Early Late Prophase I (Chromosome Prophase I number doubled) Nuclear envelope Metaphase Anaphase Telophase I I I (diploid) copyright cmassengale 76
Prophase I Early prophase üHomologs pair. üCrossing over occurs. Late prophase üChromosomes condense. üSpindle forms. üNuclear envelope fragments. copyright cmassengale 77
Tetrads Form in Prophase I Homologous chromosomes (each with sister chromatids) Join to form a TETRAD Called Synapsis copyright cmassengale 78
Crossing-Over ü Homologous chromosomes in a tetrad cross over each other ü Pieces of chromosomes or genes are exchanged ü Produces Genetic recombination in the offspring copyright cmassengale 79
Homologous Chromosomes During Crossing-Over copyright cmassengale 80
Crossing-Over Crossing-over multiplies the already huge number of different gamete types produced by copyright independent assortment 81 cmassengale
Metaphase I Homologous pairs of chromosomes align along the equator of the cell copyright cmassengale 82
Anaphase I Homologs separate and move to opposite poles. Sister chromatids remain attached at their centromeres. copyright cmassengale 83
Telophase I Nuclear envelopes reassemble. Spindle disappears. Cytokinesis divides cell into two. copyright cmassengale 84
Meiosis II Gene X Only one homolog of each chromosome is present in the cell Sister chromatids carry identical genetic information. Meiosis II produces gametes with one copy of each chromosome and thus one copy of each gene. copyright cmassengale 85
Meiosis II: Reducing Chromosome Number Prophase Metaphase II Telophase II Anaphase 4 Genetically II II Different haploid cells copyright cmassengale 86
Prophase II Nuclear envelope fragments. Spindle forms. copyright cmassengale 87
Metaphase II Chromosomes align along equator of cell. copyright cmassengale 88
Anaphase II Equator Pole Sister chromatids separate and move to opposite poles. copyright cmassengale 89
Telophase II Nuclear envelope assembles. Chromosomes decondense. Spindle disappears. Cytokinesis divides cell into two. copyright cmassengale 90
Results of Meiosis Gametes (egg & sperm) form Four haploid cells with one copy of each chromosome One allele of each gene Different combinations of alleles for different genes along the chromosome copyright cmassengale 91
Meiosis Animation copyright cmassengale 92
Gametogenesis Oogenesis or Spermatogenesis copyright cmassengale 93
Spermatogenesis ü Occurs in the testes ü Two divisions produce 4 spermatids ü Spermatids mature into sperm ü Men produce about 250, 000 sperm per day copyright cmassengale 94
Spermatogenesis in the Testes Spermatid copyright cmassengale 95
Spermatogenesis copyright cmassengale 96
Oogenesis üOccurs in the ovaries üTwo divisions produce 3 polar bodies that die and 1 egg üPolar bodies die because of unequal division of cytoplasm üImmature egg called oocyte üStarting at puberty, one oocyte matures into an ovum (egg) every 28 days copyright cmassengale 97
Oogenesis in the Ovaries copyright cmassengale 98
Oogenesis First polar body may divide (haploid) a Mitosis Oogonium (diploid) X A X X a a Polar bodies die X Primary oocyte (diploid) Meiosis II (if fertilization A occurs) X A X Secondary oocyte (haploid) copyright cmassengale Ovum (egg) Mature egg A X Second polar body (haploid) 99
Comparing Mitosis and Meiosis copyright cmassengale 100
Comparison of Divisions Mitosis Number of divisions Number of daughter cells Genetically identical? 1 Meiosis 2 2 4 Yes No Same as parent Half of parent Where Somatic cells Germ cells When Throughout life At sexual maturity Role Growth and repair copyright cmassengale Sexual reproduction Chromosome # 101
copyright cmassengale 102
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