CELLION MEETING Lund 30 th August 4 st

CELLION MEETING Lund, 30 th August – 4 st September 2006 CELLION Studies on cellular response using nanotechnology Natalia Arteaga Marrero Lund Institute of Technology Physics Department, Division of Nuclear Physics

CELLION MEETING Lund, 30 th August – 4 st September 2006 WORK IN PROGRESS/FINISHED § Vacuum guard Tested § To test target accuracy Tested and need to be improved § How to evaluate cell damage? BMC – redox state § New sample holder design Fabricated and tested § Thin film irradiation dish Tested q Search. Roi program improvement q Non-targeted cell irradiation Natalia Arteaga Marrero, Lund University

CELLION MEETING Lund, 30 th August – 4 st September 2006 Development of routines to locate cell position Search. Roi program improvement: V 79 Chinese hamster 800 x 600 pixels PHASE I: • Convert input picture to greyscale • Apply threshold filter • Darker regions are detected • Probable cells Natalia Arteaga Marrero, Lund University

CELLION MEETING Lund, 30 th August – 4 st September 2006 Development of routines to locate cell position Search. Roi program improvement: V 79 Chinese hamster 800 x 600 pixels PHASE I: • Convert input picture to greyscale • Apply threshold filter • Darker regions are detected • Probable cells Natalia Arteaga Marrero, Lund University

CELLION MEETING Lund, 30 th August – 4 st September 2006 Development of routines to locate cell position PHASE I: PHASE II: • Convert input picture to greyscale • Apply threshold filter • To study the probability distribution function of the areas of the probable cells • Detect edges • Get rid of gaps • Find probable cells Natalia Arteaga Marrero, Lund University

CELLION MEETING Lund, 30 th August – 4 st September 2006 PHASE II: • To study the probability distribution function of the areas of the probable cells Log-normal distribution Natalia Arteaga Marrero, Lund University

CELLION MEETING Lund, 30 th August – 4 st September 2006 2505 2371 Natalia Arteaga Marrero, Lund University

CELLION MEETING Lund, 30 th August – 4 st September 2006 Development of routines to locate cell position PHASE III: • R - the ratio between the area and the perimeter of each cell candidate • Assuming that cells have a preferred ratio and this is different from the R that other particles prefer • All the regions that differ from the mean by more than a certain fraction of standard deviation are also discarded Natalia Arteaga Marrero, Lund University

CELLION MEETING Lund, 30 th August – 4 st September 2006 2371 Program efficiency: 96% 4% Processing time: 0. 5 seconds Natalia Arteaga Marrero, Lund University 2219

CELLION MEETING Lund, 30 th August – 4 st September 2006 NON-TARGET IRRADIATION • Hematoma cells (Hep. G 2) • Irradiation dish: SU-8 layer (5 microns thickness) • Protons 2. 5 Me. V • The culture medium is removed prior to the irradiation • Pattern Natalia Arteaga Marrero, Lund University

CELLION MEETING Lund, 30 th August – 4 st September 2006 NON-TARGET IRRADIATION 15 x 2 = 30 4 x 3 = 12 Natalia Arteaga Marrero, Lund University 42

CELLION MEETING Lund, 30 th August – 4 st September 2006 CONCLUSION AND FUTURE WORK • The LNP has been adjusted for biological applications - Capable to control single ions - Cell recognition procedure is performing well New camera – More pictures to test the program! - Accomplish non-target irradiation • To continue non-target irradiation • To start target irradiation - New scanning system for better target accuracy - Bigger cell line Natalia Arteaga Marrero, Lund University

CELLION MEETING Lund, 30 th August – 4 st September 2006 Thanks for your attention!! Natalia Arteaga Marrero, Lund University