Cell Structure Chapter 5 Cell Theory All organisms

Cell Structure Chapter 5

Cell Theory • • • All organisms are composed of one or more cells. Cells are the smallest living units of all living organisms. Cells arise only by division of a previously existing cell. 2 2

• • • Cell Characteristics Genetic material – single circular molecule of DNA in prokaryotes – double helix located in nucleus in eukaryotes – nuclear envelope (double membrane Cytoplasm fills cell interior – Phospholipid – sugars, amino acids, – proteins - organelles Plasma membrane encloses – the cell – phospholipid bilayer Membrane proteins 3 3

Generalized Eukaryotic Cell 4 4

Cell Size • Most cells are relatively small because as size increases, volume increases much more rapidly. – longer diffusion time 5 5

Visualizing Cells • Resolution - minimum distance two points can be apart and still be distinguished as two separate points – Compound microscopes - magnify in stages using multiple lenses – Transmission electron microscope electrons transmitted through specimen – Scanning electron microscope - electrons beamed onto surface of the specimen 6 6

Visualizing Cells 7 7

Prokaryotic Cells • Simplest organisms – Cytoplasm is surrounded by plasma membrane and encased in a rigid cell wall composed of peptidoglycan. § no distinct interior compartments v gram-positive – thick single layer wall that retains a violet dye from Gram stain procedure v gram-negative – multilayered wall does not retain dye Ø Susceptibility of bacteria to antibiotics depends on cell wall structure. 8 8

Prokaryotic Cells • Some use flagellum for locomotion – threadlike structures protruding from cell surface Bacterial cell wall Rotary motor Sheath Flagellin 9 9

Eukaryotic Cells • Characterized by compartmentalization by an endomembrane system, and the presence of membrane-bound organelles. – Central vacuole – plants, storage – Vesicles (smaller) – Chromosomes - DNA and protein – Cytoskeleton (internal protein scaffolding) – Cell walls – plants and fungi 10 10

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• • Nucleus Repository for genetic material Directs activities of the cell Usually single, some cells several, RBC none – Nucleolus - region of intensive ribosomal RNA synthesis Surface of nucleus bound by two phospholipid bilayer membranes – nuclear membrane – Nuclear pores – protein gatekeepers § Usually proteins going in and RNA going out 13 13

Nucleus 14 14

Chromosomes • DNA of eukaryotes is divided into linear chromosomes. – exist as strands of chromatin, except during cell division – associated with packaging histones, packaging proteins § nucleosomes 15 15

• Endomembrane System Compartmentalizes cell, channeling passage of molecules through cell’s interior. – Endoplasmic reticulum § Rough ER - studded with ribosomes § Smooth ER - few ribosomes 16 16

Endoplasmic reticulum • • • Largest internal membrane Composed of Lipid bilayer Serves as system of channels from the nucleus Functions in storage and secretion Rough ER is “rough” because of associated ribosomes (sites of protein synthesis Smooth ER - lack associated ribosomes – contained embedded enzymes, catalyze synthesis of carbohydrate and lipid molecules 17 17

• Endomembrane System Golgi apparatus – collection of Golgi bodies § collect, package, and distribute molecules synthesized at one location in the cell and utilized at another location § Front - cis , Back – trans § Cisternae – stacked membrane folds 18 18

Copyright © The Mc. Graw-Hill Companies, Inc. Permission required for reproduction or display. Cisternae cis face Proteins trans face Golgi apparatus Transport vesicle Protein Vesicle Migrating budding transport from rough vesicle endoplasmic reticulum Ribosome Fusion of vesicle with Golgi apparatus 19 19

Endomembrane System • Vesicles – Lysosomes - membrane-bound vesicles containing digestive enzymes – from Golgi – Microbodies - enzyme-bearing, membrane -enclosed vesicles. § Peroxisomes - contain enzymes that catalyze the removal of electrons and associated hydrogen atoms § Peroxisome – named for hydrogen peroxide produced as a by-product § Enzyme breaks down to water and 20 oxygen 20

Copyright © The Mc. Graw-Hill Companies, Inc. Permission required for reproduction or display. Cytoplasm Endoplasmic reticulum Phagocytosis Food vesicle Golgi apparatus Lysosomes Plasma membrane Extracellular fluid Digestion of phagocytized food particles or cells Transport vesicle Old or damaged organelle Breakdown of old organelle 21 21

Ribosomes • Ribosomes are RNA-protein complexes composed of two subunits that join and attach to messenger RNA. – site of protein synthesis – assembled in nucleoli 22 22

Organelles With DNA • • Mitochondria – bounded by exterior and interior membranes – interior partitioned by cristae Chloroplasts – have enclosed internal compartments of stacked grana, containing thylakoids – found in photosynthetic organisms 23 23

Mitochondria A. "Powerhouse of the cell" - cellular metabolism B. Structure- outer and inner membranes, cristae C. Have their own DNA 24 24

Chloroplasts • Chloroplasts are larger and more complex than mitochondria • Grana – closed compartments of stacked membranes • Thylakoids – disc shaped structure – light capturing pigment • Stroma – fluid matrix 25 25

Endosymbiosis • Endosymbiotic theory suggests engulfed prokaryotes provided hosts with advantages associated with specialized metabolic activities. 26 26

Theory of Endosymbiosis 27 27

Evidence for the endosymbiont theory is that mitochondria and chloroplasts: - Are appropriate size to be descendants of eubacteria. - Have inner membranes similar to those on prokaryotic plasma membranes. - Replicate by splitting, as in prokaryotes. - DNA is circular and different from the DNA of the cell's nucleus. - Contain their own components for DNA transcription and translation into proteins. - Have ribosomes similar to prokaryotic ribosomes. - Molecular systematics lend evidence to support this theory. - Many extant organisms are involved in endosymbiotic relationships. 28 28

Cytoskeleton • Network of protein fibers supporting cell shape and anchoring organelles – Actin filaments Microtubules § cell movement – Microtubules Intermediate filaments § Hollow tubes § Facilitate cell movement § Centrioles – barrel shaped § organelles occur in pairs – § help assemble animal cell’s microtubules Actin – Intermediate filaments § Stable - don’t break down 29 29

Cytoskeleton 30 30

Plant Cells • • Central vacuole – often found in the center of a plant, and serves as a storage facility for water and other materials Cell wall – primary walls – laid down while cell is growing – middle lamella – glues cells together – secondary walls – inside the primary cell walls after growth 31 31

Plant Cell 32 32

Animal Cells • Animal cells lack cell walls. – form extracellular matrix § provides support, strength, and resilience 33 33

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