Cell Separation Using Magnetic Beads Mini MACS MS

Cell Separation Using Magnetic Beads

® Mini. MACS MS Column Magnet Separator

MACS® Technology • MACS Technology Used For Cell Separation – Magnetic Activated Cells Sorting • Yields Relatively High Purity Cell Populations • 3 Components Involved – MACS Micro. Beads – MACS Column – MACS Separator

® MACS Technology • MACS Micro. Beads – 50 nm in size – Biodegradable – Conjugated to monoclonal antibodies • MACS Column – Generates strong magnetic field

Labeling Of Cells With Beads Bead Monoclonal Ab

Magnetic Separation Trapped Labeled Cells High Gradient Magnetic Field

Magnetic Separation Cells Of Interest

Direct Labeling • Advantages – Fast – Yields High Purity Cell Populations • Disadvantages – Binding of antibody to surface marker may activate cell – Some cell populations may require multiple markers to be identified

Indirect Labeling

Indirect Labeling • Useful in cases with dim surface marker expression • Allows for amplification of signal • Secondary antibody recognizes – Biotin – FITC – Fc portion of primary antibody • Streptavidin instead of secondary antibody – Recognizes biotin on primary antibody

Depletion Strategy • Unwanted Cells Are Magnetically Labelled And Depleted • Useful In Following Cases – Removal of Unwanted Cells – No specific antibody is available for target cell – Binding of antibody to target cell results in activations

Depletion Strategy

Depletion Strategy

Depletion Strategy

Protocol For Isolation Of CD 4+ Cells

Protocol For Isolation Of CD 4+ Cells

Protocol For Isolation Of CD 4+ Cells