Cell Division Cycle 1 2 3 4 5

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Cell Division Cycle 1

Cell Division Cycle 1

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(cleavage) 6

(cleavage) 6

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MPF 8

MPF 8

MPF 9 M- (M-Cdk)

MPF 9 M- (M-Cdk)

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üPoint 12 “START” • Room to grow, • Sufficient nutrients, • Adhesion to substratum

üPoint 12 “START” • Room to grow, • Sufficient nutrients, • Adhesion to substratum

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ü Point üPoint 14 ü Point “START” • Room to grow, • Sufficient nutrients,

ü Point üPoint 14 ü Point “START” • Room to grow, • Sufficient nutrients, • Adhesion to substratum

G 1 -CDK G 1/S-CDK M-CDK 15

G 1 -CDK G 1/S-CDK M-CDK 15

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 • Room to grow, • Sufficient nutrients, • Adhesion to substratum G 1

• Room to grow, • Sufficient nutrients, • Adhesion to substratum G 1 -S cyclin 24 G 1 cyclin

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S- 28 p 21

S- 28 p 21

To Fully Activate a Cyclin-Dependent Kinase: • Bound to Cyclin • Phosphorylated on an

To Fully Activate a Cyclin-Dependent Kinase: • Bound to Cyclin • Phosphorylated on an Activating Site (CAK) • De-phosphorylated in an Inactivating Site (Cdc 25) • Not Bound to a Cyclin Inhibitor (INK) 29

Targets of Cyclin-Dependent Kinases: M-CDK: cdc 25 phosphatase, mitotic machinery, nuclear architecture G 1

Targets of Cyclin-Dependent Kinases: M-CDK: cdc 25 phosphatase, mitotic machinery, nuclear architecture G 1 - & G 1/S-CDK: Rb S-CDK: cdc 6, pre-replicative complex 30

proteasome 31 P

proteasome 31 P

Apoptosis 32

Apoptosis 32

Necrosis 33 Apoptosis

Necrosis 33 Apoptosis

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Caspase Cascade Initiating Caspase Executioner Caspases 36

Caspase Cascade Initiating Caspase Executioner Caspases 36

 • Active Caspases • DNA ‘Laddering’ • PS on Extracellular leaflet 37

• Active Caspases • DNA ‘Laddering’ • PS on Extracellular leaflet 37

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Intrinsic Pathway (‘suicide’) Initiator caspase OF L E E “WH ” H DEAT Bcl

Intrinsic Pathway (‘suicide’) Initiator caspase OF L E E “WH ” H DEAT Bcl 2 Survival Factor 41

Extrinsic Pathway (‘homicide’) “DISC Initiator caspase H” T A E FD O AND ACTIVATION

Extrinsic Pathway (‘homicide’) “DISC Initiator caspase H” T A E FD O AND ACTIVATION OF PROCASPASE 9 & THE INTRINSIC PATHWAY 42