Cell Biology I Overview II Membranes How Matter
Cell Biology I. Overview II. Membranes: How Matter Get in and Out of Cells III. DNA, RNA, and Chromosome Structure IV. Protein Synthesis
IV. Protein Synthesis Why is this important? Well…what do proteins DO?
IV. Protein Synthesis Why is this important? Well…what do proteins DO? Think about it this way: 1) sugars, fats, lipids, nucleic acids and proteins, themselves, are broken down and built up through chemical reactions catalyzed by enzymes. 2) So everything a cell IS, and everything it DOES, is either done by proteins or is done by molecules put together by proteins.
IV. Protein Synthesis A. Overview ATGCTGACTACTG T A C G A CT G A C Genes are read by enzymes and RNA molecules are produced… (r-RNA) this is TRANSCRIPTION UGCUGACUACU (m-RNA) (t-RNA)
IV. Protein Synthesis A. Overview ATGCTGACTACTG T A C G A CT G A C Genes are read by enzymes and RNA molecules are produced… (r-RNA) this is TRANSCRIPTION UGCUGACUACU (m-RNA) Eukaryotic RNA and some prokaryotic RNA have regions cut out… this is RNA SPLICING (t-RNA)
IV. Protein Synthesis A. Overview ATGCTGACTACTG T A C G A CT G A C UGCUGACUACU R-RNA is complexed with proteins to form ribosomes. Specific t-RNA’s bind to specific amino acids. (r-RNA) (t-RNA) Amino acid (m-RNA) ribosome
IV. Protein Synthesis A. Overview ATGCTGACTACTG T A C G A CT G A C UGCUGACUACU The ribosome reads the m-RNA. Based on the sequence of (r-RNA) nitrogenous bases in the m-RNA, a specific sequence of amino acids (carried to the ribosome by t-RNA’s) is linked together to form a protein. This is TRANSLATION. (m-RNA) ribosome (t-RNA) Amino acid
IV. Protein Synthesis A. Overview ATGCTGACTACTG T A C G A CT G A C UGCUGACUACU The protein product may be modified (have a sugar, lipid, nucleic acid, or another protein added) and/or spliced to become a functional protein. This is POST-TRANSLATIONAL MODIFICATION. (r-RNA) Amino acid (m-RNA) ribosome glycoprotein (t-RNA)
IV. Protein Synthesis A. Overview B. The Process of Protein Synthesis 1. Transcription a. The message is on one strand of the double helix - the sense strand: 3’ 5’ A C T A C G T A C A A A C G G T T A C T T T sense T G A T G C A T G T T T G C C A A T G A A A nonsense 5’ 3’ “TAG A CAT” message makes ‘sense’ “ATC T GTA” ‘nonsense’ limited by complementation
IV. Protein Synthesis A. Overview B. The Process of Protein Synthesis 1. Transcription a. The message is on one strand of the double helix - the sense strand: 3’ 5’ A C T A C G T A C A A A C G G T T A C T T T sense T G A T G C A T G T T T G C C A A T G A A A nonsense 5’ 3’ exon intron exon In all eukaryotic genes and in some prokaryotic sequences, there are introns and exons. There may be multiple introns of varying length in a gene. Genes may be several thousand base-pairs long. This is a simplified example!
IV. Protein Synthesis A. Overview B. The Process of Protein Synthesis 1. Transcription b. The cell 'reads' the correct strand based on the location of the promoter, the antiparallel nature of the double helix, and the chemical limitations of the 'reading' enzyme, RNA Polymerase. 3’ Promoter 5’ A C T A C G T A C A A A C G G T T A C T T T sense T G A T G C A T G T T T G C C A A T G A A A nonsense 5’ 3’ exon intron exon Promoters have sequences recognized by the RNA Polymerase. They bind in particular orientation.
IV. Protein Synthesis A. Overview B. The Process of Protein Synthesis 1. Transcription b. The cell 'reads' the correct strand based on the location of the promoter, the antiparallel nature of the double helix, and the chemical limitations of the 'reading' enzyme, RNA Polymerase. 3’ Promoter 5’ A C T A C G T A C A A A C G G T T A C T T T sense G C A U GUUU G C C A A U AUG A U G A T A T G C A T G T T T G C C A A T G A A A nonsense 5’ 3’ exon intron exon 1) Strand separate 2) RNA Polymerase can only synthesize RNA in a 5’ 3’ direction, so they only read the anti-parallel, 3’ 5’ strand (‘sense’ strand).
IV. Protein Synthesis A. Overview B. The Process of Protein Synthesis 1. Transcription c. Transcription ends at a sequence called the 'terminator'. 3’ Promoter Terminator 5’ A C T A C G T A C A A A C G G T T A C T T T sense G C A U GUUU G C C A A U AUG A U G A T A T G C A T G T T T G C C A A T G A A A nonsense 5’ 3’ exon intron exon Terminator sequences destabilize the RNA Polymerase and the enzyme decouples from the DNA, ending transcription
IV. Protein Synthesis A. Overview B. The Process of Protein Synthesis 1. Transcription c. Transcription ends at a sequence called the 'terminator'. 3’ Promoter Terminator 5’ A C T A C G T A C A A A C G G T T A C T T T sense G C A U GUUU G C C A A U AUG A U G A T A T G C A T G T T T G C C A A T G A A A nonsense 5’ 3’ exon Initial RNA PRODUCT: intron exon
IV. Protein Synthesis A. Overview B. The Process of Protein Synthesis 1. Transcription c. Transcription ends at a sequence called the 'terminator'. 3’ Promoter Terminator 5’ A C T A C G T A C A A A C G G T T A C T T T sense T G A T G C A T G T T T G C C A A T G A A A nonsense 5’ 3’ exon Initial RNA PRODUCT: intron exon G C A U GUUU G C C A A U AUG A U G A
IV. Protein Synthesis A. Overview B. The Process of Protein Synthesis 1. Transcription 2. Transcript Processing exon Initial RNA PRODUCT: intron exon G C A U GUUU G C C A A UAUG A U G A Introns are spliced out, and exons are spliced together. Sometimes these reactions are catalyzed by the intron, itself, or other catalytic RNA molecules called “ribozymes”.
IV. Protein Synthesis A. Overview B. The Process of Protein Synthesis 1. Transcription 2. Transcript Processing intron exon Final RNA PRODUCT: exon AUG A G C A U GUUU G C C A A U U G A This final RNA may be complexed with proteins to form a ribosome (if it is r-RNA), or it may bind amino acids (if it is t-RNA), or it may be read by a ribosome, if it is m. RNA and a recipe for a protein.
IV. Protein Synthesis A. Overview B. The Process of Protein Synthesis 1. Transcription 2. Transcript Processing 3. Translation a. m-RNA attaches to the ribosome at the 5' end. M-RNA: G C A U G U U U G C C A A UU G A
IV. Protein Synthesis A. Overview B. The Process of Protein Synthesis 1. Transcription 2. Transcript Processing 3. Translation a. m-RNA attaches to the ribosome at the 5' end. M-RNA: G C A U G U U U G C C A A UU G A It then reads down the m-RNA, one base at a time, until an ‘AUG’ sequence (start codon) is positioned in the first reactive site.
IV. Protein Synthesis A. Overview B. The Process of Protein Synthesis 1. Transcription 2. Transcript Processing 3. Translation a. m-RNA attaches to the ribosome at the 5' end. b. a specific t-RNA molecule, with a complementary UAC anti-codon sequence, binds to the m-RNA/ribosome complex. Meth M-RNA: G C A U G U U U G C C A A UU G A
IV. Protein Synthesis A. Overview B. The Process of Protein Synthesis 1. Transcription 2. Transcript Processing 3. Translation a. m-RNA attaches to the ribosome at the 5' end. b. a specific t-RNA molecule, with a complementary UAC anti-codon sequence, binds to the m-RNA/ribosome complex. c. A second t-RNA-AA binds to the second site Phe Meth M-RNA: G C A U G U U U G C C A A UU G A
IV. Protein Synthesis A. Overview B. The Process of Protein Synthesis 1. Transcription 2. Transcript Processing 3. Translation a. m-RNA attaches to the ribosome at the 5' end. b. a specific t-RNA molecule, with a complementary UAC anti-codon sequence, binds to the m-RNA/ribosome complex. c. A second t-RNA-AA binds to the second site d. Translocation reactions occur Meth M-RNA: Phe G C A U G U U U G C C A A UU G A The amino acids are bound and the ribosome moves 3 -bases “downstream”
IV. Protein Synthesis A. Overview B. The Process of Protein Synthesis 1. Transcription 2. Transcript Processing 3. Translation e. polymerization proceeds Meth M-RNA: Ala Asn Phe G C A U G U U U G C C A A UU G A The amino acids are bound and the ribosome moves 3 -bases “downstream”
IV. Protein Synthesis A. Overview B. The Process of Protein Synthesis 1. Transcription 2. Transcript Processing 3. Translation e. polymerization proceeds Meth M-RNA: Asn Phe Ala G C A U G U U U G C C A A UU G A The amino acids are bound and the ribosome moves 3 -bases “downstream”
IV. Protein Synthesis A. Overview B. The Process of Protein Synthesis 1. Transcription 2. Transcript Processing 3. Translation e. polymerization proceeds f. termination of translation Meth M-RNA: Phe Ala Asn G C A U G U U U G C C A A UU G A Some 3 -base codon have no corresponding t-RNA. These are stop codons, because translocation does not add an amino acid; rather, it ends the chain.
IV. Protein Synthesis A. Overview B. The Process of Protein Synthesis 1. Transcription 2. Transcript Processing 3. Translation 4. Post-Translational Modifications Meth Phe Ala Asn Most initial proteins need to be modified to be functional. Most need to have the methionine cleaved off; others have sugar, lipids, nucleic acids, or other proteins are added.
IV. Protein Synthesis A. Overview B. The Process of Protein Synthesis C. Regulation of Protein Synthesis 1. Regulation of Transcription - DNA bound to histones can’t be accessed by RNA Polymerase - but the location of histones changes, making genes accessible (or inaccessible) Initially, the orange gene is “off”, and the green gene is “on” Now the orange gene is “on” and the green gene is “off”.
IV. Protein Synthesis A. Overview B. The Process of Protein Synthesis C. Regulation of Protein Synthesis 1. Regulation of Transcription 3’ Promoter 5’ A C T A C G T A C A A A C G G T T A C T T T sense T G A T G C A T G T T T G C C A A T G A A A nonsense 5’ 3’ exon RNA POLY intron exon Transcription factors can inhibit or encourage the binding of the RNA Polymerase. And, through signal transduction, environmental factors can influence the activity of these transcription factors. So cells can respond genetically to changes in their environment.
IV. Protein Synthesis A. Overview B. The Process of Protein Synthesis C. Regulation of Protein Synthesis 1. Regulation of Transcription 2. Transcript Processing Initial RNA PRODUCT: Cut not made exon intron exon G C A U GUUU G C C A A UAUG A UAUA Mi-RNA’s and si-RNA’s are small RNA molecules that can bind to m-RNA and disrupt correct spicing, creating non-functional m-RNA’s.
IV. Protein Synthesis A. Overview B. The Process of Protein Synthesis C. Regulation of Protein Synthesis 1. Regulation of Transcription 2. Transcript Processing 3. Regulating Translation Meth M-RNA: Phe G C A U G U U G A A A UU G A Incorrect splicing can result in a ‘premature’ stop codon, terminating translation early, resulting in a non-functional protein.
IV. Protein Synthesis A. Overview B. The Process of Protein Synthesis C. Regulation of Protein Synthesis 1. Regulation of Transcription 2. Transcript Processing 3. Regulating Translation 4. Regulating Post-Translational Modification Meth Phe The patterns of cleavage and modification can vary. Ala Asn
IV. Protein Synthesis A. Overview B. The Process of Protein Synthesis C. Regulation of Protein Synthesis 1. Regulation of Transcription 2. Transcript Processing 3. Regulating Translation 4. Regulating Post-Translational Modification Affected by other cells Affected by other genes Affected by the environment Protein ? Gene activity is responsive to cellular and environmental cues
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