CDCNIH Vertebrate Animal Biosafety Level Criteria For all
CDC/NIH Vertebrate Animal Biosafety Level Criteria ► For all Animal Biosafety Levels (1 - 4) § IACUC Approval § Authorized access to facilities § Animal handling training § Medical surveillance program § Written safety manual(s) § Pest control program
CDC/NIH Verterbrate Animal Biosafety Level Criteria ► For all Animal Biosafety Levels (1 - 4) § no eating, drinking, smoking, touching face § no food or drink storage within facilities § minimize aerosols § decontaminate work surfaces, equipment § transport wastes in leak-proof covered containers § handle sharps safely
CDC/NIH Verterbrate Animal Biosafety Level Criteria ► For all Animal Biosafety Levels (1 - 4) § Protective clothing recommended, not worn outside facility § Facilities separate from general building traffic § External doors self-closing/self-locking § Animal room doors open inward, self-closing § Water resistant, easily cleaned surfaces (horizontal surfaces kept to a minimum)
CDC/NIH Verterbrate Animal Biosafety Level Criteria ► For all Animal Biosafety Levels (1 - 4) § Sealed, break resistant windows § Floor drain traps filled with water or disinfectant § No recirculation of exhaust air § Rooms have negative pressure gradient to adjacent hallway § Hand washing sink available in facility § 180 F cage wash rinse temperature § Adequate illumination
CDC/NIH Verterbrate Animal Biosafety Level Criteria ► Animal Biosafety Level 2 (ABSL 2) § Restrict access to few as possible (advise support staff of potential hazards) § Biohazard sign posted on animal room entrance (hazard ID, contact info. , & entry requirements) § Immunizations, serum surveillance § Labeled leakproof carriers, exterior disinfected before transport (wastes, tissues, etc. ) § Safe sharps policies (plasticware, safe sharps devices, limited use of needles/syringes)
CDC/NIH Verterbrate Animal Biosafety Level Criteria ► Animal Biosafety Level 2 (ABSL 2) § Limited to animals dedicated to work § Exposures, spills reported immediately § Gloves, gowns, uniforms or lab coats worn in rooms (removed prior to exit) § Face protection (goggles, safety glasses, full face shields, masks) selected on basis of risk § Biosafety cabinets used to confine aerosol procedures
CDC/NIH Verterbrate Animal Biosafety Level Criteria ► Animal Biosafety Level 2 (ABSL 2) § Filter top cages, cage dumping containment stations used where appropriate § Autoclave available § hand washing sink in animal room
CDC/NIH Verterbrate Animal Biosafety Level Criteria ► Animal Biosafety Level 3 (ABSL 3) § Individuals at increased risk of infection not allowed in facility § All wastes treated before disposal as regulated biomedical waste (incineration recommended) § Specialized training provided and updated § Cages autoclaved/decontaminated before bedding is removed § Spill procedures posted
CDC/NIH Verterbrate Animal Biosafety Level Criteria ► Animal Biosafety Level 3 (ABSL 3) § Uniforms or scrub suits covered by solidfront gown § Face/eye, and where necessary, respiratory protection worn by all who enter animal rooms § Boots, shoe covers, etc. worn when needed § Consider use of containment ventilated cage units, housing in biosafety cabinets, of filter bonnets.
CDC/NIH Verterbrate Animal Biosafety Level Criteria ► Animal Biosafety Level 3 (ABSL 3) § All procedures performed in biosafety cabinet (wear respiratory protection if working outside cabinet) § Consider clothes change room and shower § Penetrations in rooms are sealed or capable of being sealed § Hands-free sink near exit door § Sink-trap filled with disinfectant after each use
CDC/NIH Verterbrate Animal Biosafety Level Criteria ► Animal Biosafety Level 3 (ABSL 3) Ducted exhaust ventilation system Exhaust discharge away from air intakes, Consider HEPA filtration of exhaust air Room airflow monitoring device (personnel verify airflow direction upon entry) § Consider alarms for ventilation system failure § Consider interlock of exhaust/supply systems to avoid pressurization § §
CDC/NIH Verterbrate Animal Biosafety Level Criteria ► Animal Biosafety Level 3 (ABSL 3) § Biosafety cabinets tested/certified annually § Autoclave located near BL 3 suite § Vacuum system protected with HEPA filter if used § Initial and annual verification of BL 3 facility design parameters § Consider additional facility enhancements as needed (effluent decontamination, showers, etc)
CDC/NIH Verterbrate Animal Biosafety Level Criteria ► Animal Biosafety Level 4 (ABSL 4) § Program includes post-exposure counseling and prophylaxis § Site-specific biosafety manual prepared § Double-door pass through autoclave § Procedures performed in pairs § Materials unrelated to work not allowed in facility § Additional access control measures (security guard)
CDC/NIH Verterbrate Animal Biosafety Level Criteria ► Animal Biosafety Level 4 (ABSL 4) § Complete clothes change, personal shower upon exit § All material in/out of facility through pass through autoclave or disinfectant chamber § Autoclave door interlocked to prevent both open simultaneously § Chart exposures, accidents/incidents, employee absenteeism, appearance of related signs/symptoms of infection
CDC/NIH Verterbrate Animal Biosafety Level Criteria ► Animal Biosafety Level 4 (ABSL 4) § Serum samples analyzed at defined intervals § Positive pressure suits or Class III biosafety cabinet line § Very unique engineering requirements for BL 4 laboratories (dedicated building within building concept)
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