CASIROZ participant 8 assistant contractor to participant 7

CASIROZ – participant 8 assistant contractor to participant 7 Michael Tausz, Dieter Grill Maria Müller Karin Herbinger, Klaus Remele Institut für Pflanzenphysiologie, Universität Graz, Schubertstraße 51, A-8010 Graz, Austria

Planned investigations • Biochemical defense – Ascorbate system – Glutathione system – Tocopherol – Chloroplast pigments • Chromosomal damages – Aberration rates

Symplast Apoplast Photosynthetic apparatus ROS NADPH NADP GSH GSSG NADPH GSH NADP GSSG Asc Plasmalemma Chloroplast Secondary products O 3 Cell wall ROS DHAsc Secondary products Asc DHAsc

Aberration types – sticky chromosomes anaphase bridges

Further aberrations – vagrant chromosomes in – metaphase anaphase

Planned experiments • Biochemical defense – Ascorbate system – Glutathione system – Tocopherol – Chloroplast pigments • Chromosomal damages – Aberration rates

Experiments • Container exposed young trees • Branch cuvette exposures • Mature trees

Container exposed young trees • Ascorbate; glutathione; tocopherols; pigments in leaves of young (3 -yrs-old) trees (exposed at 2 canopy levels). • Ascorbate; glutathione; tocopherols in roots of young (3 -yrs-old) trees (exposed at 2 canopy levels). Depends on available material - possible only once per season! • Chromosomal aberration analysis in roots of young (3 -yrs-old) trees (exposed at 2 canopy levels).

Branch cuvette exposures • Ascorbate; glutathione; tocopherols; pigments in leave samples included in branch cuvette fumigation systems (interface to partner 7).

Mature trees • Tocopherols and chloroplast pigments in leaves of adult trees (interface to partner 4 who contributes glutathione and ascorbate). Sampling schedule partner 4. • Ascorbate; glutathione; tocopherols in roots of adult trees. Sampling schedule depending on available root material - possibly once a season. • Chromosomal aberration analysis in roots of adult trees.