Carl Henrik Grbitz Ute Krengel Department of Chemistry

Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, Ui. O

Crystal engineering Biomarker identification Protein crystallography

Molecules H 2 ethane sucrose

Molecules

Molecules

Crystal

Crystal ”Crystal engineering”, we make a supermolecule!

Building blocks node linker

Problem 1 Molecules are very small, hard to put together one by one Xe-atoms on Ni-surface (Don Eigler, 1989)

Problem 2 As they are so small, we must put together very many before we reach a macroscopic size A 1 x 1 mm sugar crystal contains 1, 4 · 1018 molecules

Building blocks

Building blocks

Glue

Intermolecular forces Must be: n n strong Directional Two main types used in CE: n n Hydrogen bonds Metal coordination

MOFs n “Metal-Organic Frameworks (MOFs) are crystalline compounds consisting of metal ions or clusters coordinated to often rigid organic molecules to form one-, two-, or threedimensional structures that can be porous” (from Wikipedia) MOF-5

Can we do this without metal ions?

Guanidinium derivatives Fumaric acid complex

Guanidinium derivatives

DNA-based systems

DNA-based systems

DNA-based systems

Applications Construction of n n n n n polar materials for non-linear optics molecular magnets porous materials for storage molecular sieves sensors molecule traps biological model systems catalysts and much more

Biomarker identification Biomarker: A biological molecule found in blood, other body fluids, or tissues that is a sign of a normal or abnormal process, or of a condition or disease. A biomarker may be used to see how well the body responds to a treatment for a disease or condition. Also called molecular marker and signature molecule Present in small amounts = hard to identify

Biomarker identification Nano-HPLC-system

Group Lianglin Lise-Lotte Carl Henrik Malgorzata Fassil Steven Ola

Protein crystallography

Structure/Function Studies of Medically Relevant Systems Department of Chemistry University of Oslo Ute Krengel

Methods X-ray crystallography Complementary techniques (molecular biology, protein chemistry, ligand binding studies, cell biology, molecular docking, organic chemistry)

Targets Glycobiological Targets Enzymes ◦ Chorismate mutases Glyco. Nor Prot. Struct ◦ Mucins ◦ Bacterial Toxins ◦ Bacterial Adhesins ◦ Anti-tumor Antibodies ◦ Mushroom Lectins

Masters Projects Glycobiological Targets: ◦ Bacterial Toxins – Structure and Function Investigation of blood group dependence Delivery mechanisms? Drug design ◦ Anti-tumor Antibodies for Immunotherapy Recombinant production, crystallization and X-ray structure determination

Group Ute Øyvind Dani Hedda Daniel Julie Dipankar