Campbell Essential Biology Seventh Edition and Campbell Essential

Campbell Essential Biology, Seventh Edition, and Campbell Essential Biology with Physiology, Sixth Edition Chapter 06 Cellular Respiration: Obtaining Energy from Food Power. Point® Lectures created by Edward J. Zalisko, Eric J. Simon, Jean L. Dickey, and Jane B. Reece Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

The Cells of Your Brain Burn Through a Quarter Pound of Glucose Each Day

Biology and Society: Getting the Most Out of Your Muscles (1 of 2) • For many endurance athletes, the rate at which oxygen (O 2) is provided to working muscles is the limiting factor in their performance. • Your aerobic capacity is – the maximum rate at which O 2 can be taken in and used by your muscle cells and – therefore the most strenuous exercise that your body can maintain aerobically. Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

The Science of Exercise Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights

Biology and Society: Getting the Most Out of Your Muscles (2 of 2) • If you work even harder and exceed your aerobic capacity, – the demand for oxygen in your muscles will outstrip your body’s ability to deliver it, – metabolism then becomes anaerobic, and – your muscle cells switch to an “emergency mode” in which they § break down glucose very inefficiently and § produce lactic acid as a by-product. Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

Energy Flow and Chemical Cycling in the Biosphere • All life requires energy. – In almost all ecosystems on Earth, this energy originates from the sun. – During photosynthesis, plants convert the energy of sunlight to the chemical energy of sugars and other organic molecules. – All animals depend on this conversion for food and more. Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

Producers and Consumers (1 of 3) • Copyright © 2019, 2016, 2013 Pearson Education,

Producers and Consumers (2 of 3) • Most ecosystems depend entirely on photosynthesis for food. • Biologists refer to – plants and other autotrophs as producers and – heterotrophs, in contrast, as consumers, because they obtain their food by eating plants or by eating animals that have eaten plants. Checkpoint: What chemical ingredients do plants require from the environment to synthesize their own food? Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

Producer and Consumer (3 of 3) Copyright © 2019, 2016, 2013 Pearson Education, Inc.

Chemical Cycling between Photosynthesis and Cellular Respiration (1 of 3) • Copyright © 2019,

Energy Flow and Chemical Cycling in Ecosystems Copyright © 2019, 2016, 2013 Pearson Education,

Chemical Cycling between Photosynthesis and Cellular Respiration (2 of 3) • Copyright © 2019,

Chemical Cycling between Photosynthesis and Cellular Respiration (3 of 3) • Plants usually make more organic molecules than they need for fuel. This photosynthetic surplus – provides material for the plant to grow or – can be stored, as starch in potatoes, for example. Checkpoint: What is misleading about the following statement? “Plants perform photosynthesis, whereas animals perform cellular respiration. ” Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

Identifying Major Themes (1 of 3) Cellular respiration and photosynthesis are linked, with each process using inputs created by the other. Which major theme is illustrated by this action? 1. 2. 3. 4. 5. The relationship of structure to function Information flow Pathways that transform energy and matter Interactions within biological systems Evolution Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

Cellular Respiration: Aerobic Harvest of Food Energy • Copyright © 2019, 2016, 2013 Pearson

How Breathing is Related to Cellular Respiration Copyright © 2019, 2016, 2013 Pearson Education,

An Overview of Cellular Respiration (1 of 7) • All living organisms depend on transformations of energy and matter. • Cellular respiration – consists of many chemical steps using a specific enzyme to catalyze each reaction, – constitutes one of the most important metabolic pathways for nearly every eukaryotic cell, and – provides the energy these cells need to maintain the functions of life. Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

An Overview of Cellular Respiration (2 of 7) Copyright © 2019, 2016, 2013 Pearson

An Overview of Cellular Respiration (3 of 7) • The many chemical reactions that make up cellular respiration can be grouped into three main stages: 1. glycolysis, 2. the citric acid cycle, and 3. electron transport. Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

A Road Map for Cellular Respiration Copyright © 2019, 2016, 2013 Pearson Education, Inc.

An Overview of Cellular Respiration (4 of 7) • Copyright © 2019, 2016, 2013

An Overview of Cellular Respiration (5 of 7) 1 and 2. Glycolysis and the citric acid cycle generate a small amount of ATP directly and much more ATP indirectly, via reactions that transfer electrons from fuel molecules to a molecule called NAD+ (nicotinamide adenine dinucleotide). – The electron transfer forms a molecule called NADH, which acts as a shuttle carrying highenergy electrons from one area of the cell to another. Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

An Overview of Cellular Respiration (6 of 7) 3. The third stage of cellular respiration is electron transport. – Electrons captured from food by NADH are stripped of their energy, a little bit at a time, until they are finally combined with oxygen to form water. – The proteins and other molecules that make up electron transport chains are embedded within the inner membrane of the mitochondria. – Electron transport from NADH to oxygen releases the energy your cells use to make most of their ATP. Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

An Overview of Cellular Respiration (7 of 7) • Checkpoint: Which stages of cellular respiration take place in the mitochondria? Which stage takes place outside the mitochondria? Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

Stage 1: Glycolysis • During glycolysis a six-carbon glucose molecule is split in half to form two molecules of pyruvic acid. – This initial split requires an energy “investment” of two ATP molecules per glucose. – The three-carbon molecules then donate highenergy electrons to NAD+, forming NADH. – Glycolysis also generates four ATP molecules. – Glycolysis thus produces a net gain of two molecules of ATP per molecule of glucose. Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

Glycolysis Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

Stage 2: The Citric Acid Cycle (1 of 3) • Copyright © 2019, 2016,

Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

Stage 2: The Citric Acid Cycle (2 of 3) • Copyright © 2019, 2016,

The Citric Acid Cycle Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights

Stage 2: The Citric Acid Cycle (3 of 3) • Copyright © 2019, 2016,

Stage 3: Electron Transport (1 of 4) • During cellular respiration, the electrons gathered from food molecules gradually “fall, ” losing energy at each step. – Electrons are transferred from glucose in food molecules to NAD+. – This electron transfer converts NAD+ to NADH. – Then NADH releases two electrons that enter an electron transport chain, a series of electron carrier molecules. With each exchange, the electron gives up a bit of energy. Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

Cellular Respiration Illustrated Using a Hard-Hat Analogy Copyright © 2019, 2016, 2013 Pearson Education,

Stage 3: Electron Transport (2 of 4) • The overall effect of all this transfer of electrons during cellular respiration is a “downward” trip for electrons – from glucose, – to NADH, – to an electron transport chain, and – to oxygen. Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

The Role of Oxygen in Harvesting Food Energy Copyright © 2019, 2016, 2013 Pearson

Stage 3: Electron Transport (3 of 4) • The molecules of electron transport chains are built into the inner membranes of mitochondria. • Because these membranes are highly folded, their large surface area can accommodate thousands of copies of the electron transport chain—a good example of how biological structure fits function. • The energy stored by electron transport behaves something like the water behind a dam. Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

The Mitochondrion: Site of Cellular Respiration Copyright © 2019, 2016, 2013 Pearson Education, Inc.

Stage 3: Electron Transport (4 of 4) • Mitochondria have structures that act like turbines. • Each of these miniature machines, called an ATP synthase, is constructed from proteins built into the inner mitochondrial membrane, adjacent to the proteins of the electron transport chains. • Figure 6. 10 shows a simplified view of how the energy previously stored in NADH and FADH 2 can now be used to generate ATP. Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

How Electron Transport Drives ATP Synthase Machines Copyright © 2019, 2016, 2013 Pearson Education,

Identifying Major Themes (2 of 3) The highly folded membranes of the mitochondria make these organelles well suited to carry out the huge number of chemical reactions required for cellular respiration to proceed. Which major theme is illustrated by this action? 1. 2. 3. 4. 5. The relationship of structure to function Information flow Pathways that transform energy and matter Interactions within biological systems Evolution Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

The Results of Cellular Respiration (1 of 2) • Cellular respiration can generate up to 32 molecules of ATP per molecule of glucose. • Figure 6. 11 will help you keep track of the ATP molecules generated. Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

A Summary of ATP Yield During Cellular Respiration Copyright © 2019, 2016, 2013 Pearson

The Results of Cellular Respiration (2 of 2) • Respiration is a versatile metabolic furnace that can “burn” many other kinds of food molecules. • Figure 6. 12 diagrams some metabolic routes for the use of carbohydrates, fats, and proteins as fuel for cellular respiration. • The interplay between these pathways provides a clear example of theme of system interactions; in this case, all of these interactions contribute to maintaining a balanced metabolism. Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

Energy From Food Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

Identifying Major Themes (3 of 3) Your body uses many different intersecting chemical pathways that, all together, constitute your metabolism. Which major theme is illustrated by this action? 1. 2. 3. 4. 5. The relationship of structure to function Information flow Pathways that transform energy and matter Interactions within biological systems Evolution Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

Fermentation: Anaerobic Harvest of Food Energy • Some of your cells can work for short periods without oxygen. This anaerobic (“without oxygen”) harvest of food energy is called fermentation. • Fermentation relies on glycolysis, the first stage of cellular respiration. • Glycolysis does not require O 2 but does produce two ATP molecules for each glucose molecule broken down to pyruvic acid. Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

Fermentation in Human Muscle Cells • To harvest food energy during glycolysis, NAD+ must be present to receive electrons. – This is no problem under aerobic conditions, because the cell regenerates NAD+ when NADH drops its electron cargo down electron transport chains to O 2. – However, this recycling of NAD+ cannot occur under anaerobic conditions because there is no O 2. Instead, NADH disposes of electrons by adding them to the pyruvic acid produced by glycolysis, producing a waste product called lactic acid. Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

Fermentation: Producing Lactic Acid Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights

The Process of Science: What Causes Muscle Burn? (1 of 3) • Background: What causes muscles to burn after hard exercise? This question was investigated by one of the founders of the field of exercise science, a British biologist named A. V. Hill, who won a 1922 Nobel Prize for his investigations of muscle contraction. • Hill knew that muscles produce lactic acid under anaerobic conditions. In 1929, Hill developed a technique for electrically stimulating dissected frog muscles in a laboratory solution. Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

A. V. Hill’s 1929 Apparatus for Measuring Muscle Fatigue Copyright © 2019, 2016, 2013

The Process of Science: What Causes Muscle Burn? (2 of 3) • Method: – Hill showed that when lactic acid could not diffuse away from muscle tissue, muscle performance declined. – When lactic acid could diffuse away from the muscle tissue, muscle performance improved significantly. Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

The Process of Science: What Causes Muscle Burn? (3 of 3) • Results: Gradually, however, evidence that contradicted Hill’s results began to accumulate. – The effect that Hill demonstrated did not appear to occur at human body temperature. – Further, certain individuals who are unable to accumulate lactic acid have muscles that fatigue more rapidly, the opposite of what is expected. – Some research indicates that increased levels of other ions may be to blame. – Therefore, the cause of muscle fatigue remains hotly debated. Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

Fermentation in Microorganisms • Yeast and some other organisms can survive with or without O 2. • Wastes from fermentation can be ethyl alcohol, lactic acid, or other compounds, depending on the species. Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

Fermentation: Producing Ethyl Alcohol Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights

Evolution Connection: The Importance of Oxygen (1 of 2) • Aerobic and anaerobic respiration start with glycolysis. Glycolysis is thus the universal energyharvesting process of life. • The role of glycolysis in respiration and fermentation has an evolutionary basis. • Between 3. 5 and 2. 7 billion years ago, before significant levels of oxygen were present in Earth’s atmosphere, ancient prokaryotes probably used glycolysis to make ATP and generated ATP exclusively from glycolysis. Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved

A Time Line of Oxygen and Life on Earth Copyright © 2019, 2016, 2013

Evolution Connection: The Importance of Oxygen (2 of 2) • The fact that glycolysis occurs in almost all organisms suggests that it evolved very early in ancestors common to all the domains of life. • The location of glycolysis within the cell also implies great antiquity. The pathway does not require any of the membrane-enclosed organelles of the eukaryotic cell, which evolved more than a billion years after the prokaryotic cell. Copyright © 2019, 2016, 2013 Pearson Education, Inc. All Rights Reserved