Brucella ovis and Brucella melitensis serology in sheep

Brucella ovis and Brucella melitensis serology in sheep and goat The 3. Workshop of the EU Brucellosis NRL Portugal September 24 & 25 2009 by Anna-Bodil Christoffersen National Veterinary Institute, DTU, Denmark

Population of sheep and goat in Denmark • 2004: 117. 008 • 2004: 7. 577 • 2006: 126. 438 • 2006: 12. 028

Other populations in 2008 • • • Cattle: 665. 158 Dairy cattle: 557. 978 Meat cattle: 107. 180 • Bull & bullock: 53. 712 • • • Horse: 60. 029 Pig: 12. 000 (2009) Sl. Pig: 3. 015. 000 (2009) Export of pig: 600. 000 /month Human: 5. 515. 287 (2009)

B. melitensis by RBT sheep / goat 2007 4000 3392 3500 3000 2500 Submissions Positive 2000 Samples Positive 1500 1000 484 500 0 0 1 0 5 0 1 0 66 0 3 0 17 0 1 1 0 Export Import Abroad Diagnostic Surveillance

B. melitensis by RBT sheep / goat 2008 4000 3556 3500 3000 Submissions 2500 Positive 2000 Samples Positive 1500 1000 534 500 2 0 5 0 0 0 3 0 98 0 1 0 0 Export Import Abroad Diagnostic Surveillance

B. melitensis by RBT sheep / goat 2009 180 161 160 140 120 Submissions 100 Positive Samples 80 Positive 60 40 12 20 2 0 20 16 0 0 0 0 0 1 0 0 0 Export Import Abroad Diagnostric Surveillance

Summary B. melitensis • 2007: total tested 3. 480 1 pos (FPSR) • 2008: total tested 3. 660 0 pos • 2009 (until July): total tested 189 0 pos • Notifiable disease • The government pay for the surveillance

B. ovis serology by CFT in sheep / goat 2007 450 394 400 350 300 Submissions 250 Positive Samples 200 Positive 150 100 50 31 7 0 1 Export 2 0 0 0 Import 0 7 1 Abroad 9 9 18 1 1 Diagnostic 0 0 Surveillance

B. ovis serology by CFT in sheep / goat 2008 300 274 250 200 Submissions Positive 150 Samples Positive 100 50 32 7 16 0 0 0 1 1 3 0 6 0 0 0 Export Import Abroad Diagnostic Surveillance

B. ovis serology by CFT in sheep / goat 2009 90 79 80 70 60 Submissions Positive 50 Samples 40 Positive 30 20 16 10 0 7 0 0 0 Export 0 0 Import 0 1 0 Abroad 0 1 0 0 Diagnostic 2 0 0 Surveillance

Summary B. ovis ( sheep) • 2007: total 443 pos 12 (2, 7%) (abroad: 9) • 2008: total 312 pos 1 (0. 32%) (abroad: 1) • 2009 (until July): total 102 • Not notifiable disease • The owner pay for the test pos 0

Conclusions about B. melitensis • Denmark: Official free of Brucellosis in sheep/goat in 1995. • 1 -2 FPSR yearly • The official surveillance program for B. melitensis is valuated at the moment.

Conclusions about B. ovis ( ovine epididymitis) • No official surveillance program so far. • Sero positive reactions every year from Danish and samples abroad. • Seropositive: 2, 7%-0, 32% in CFT • Not ever detected by cultivation in Denmark (? ). • Importance of B. ovis/ ovine epididymitis ? • Farmers are interested in surveillance ( public task or funds ? )